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Literature summary for 3.2.1.176 extracted from
- Cellulose hydrolysis and binding with Trichoderma reesei Cel5A and Cel7A and their core domains in ionic liquid solutions (2014), Biotechnol. Bioeng., 111, 726-733.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,3-dimethylimidazolium dimethylphosphate | complete inhibition at 30% v/v, ionic liquids inactivate cellulases, the enzyme is sensitive against 1,3-dimethylimidazolium dimethylphosphate and 1-ethyl-3-methylimidazolium acetate, effects on cellulase substrate binding, overview | Trichoderma reesei |  |
| 1-ethyl-3-methylimidazolium acetate | complete inhibition at 30% v/v, ionic liquids inactivate cellulases, the enzyme is sensitive against 1,3-dimethylimidazolium dimethylphosphate and 1-ethyl-3-methylimidazolium acetate, effects on cellulase substrate binding, overview | Trichoderma reesei | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma reesei | G0RVK1 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-beta-D-lactoside + H2O | - |
Trichoderma reesei | 4-methylumbelliferol + D-lactose | - |
? | |
| microcrystalline cellulose + H2O | - |
Trichoderma reesei | ? | - |
? | |
| additional information | enzyme Cel7A hydrolyses the cellulose from the reducing chain end in a processive manner | Trichoderma reesei | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cel7A | - |
Trichoderma reesei |
| cellobiohydrolase | - |
Trichoderma reesei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
assay at | Trichoderma reesei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Trichoderma reesei |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the core domain is able to bind tightly to cellulose, even in absence of the carbohydrate-binding module. Family 1 carbohydrate-binding modules are small wedge-shaped domains with a rough and a flat face. Carbohydrate-binding module interaction with crystalline cellulose surfaces takes place through three tyrosine residues aligned on the flat face forming hydrogen bonds with the cellulose substrate | Trichoderma reesei |
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