Literature summary for 3.2.1.176 extracted from

Barr, B.K.; Hsieh, Y.L.; Ganem, B.; Wilson, D.B
Identification of two functionally different classes of exocellulases (1996), Biochemistry, 35, 586-592.

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Organism

Organism	UniProt	Comment	Textmining
Thermobifida fusca	-	-	-
Trichoderma reesei	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Trichoderma reesei
-	Thermobifida fusca

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cellopentaose + H2O	cellobiose is preferentially released from the reducing end by CBH I (72%), cellopentaose is labeled at the reducing end with (18)O, the products are analyzed by ionspray mass spectrometry. This value is approximate since IS-MS is unable to detect glucose under the conditions used. As a result, this value is based only on the amount of (18)O present in CTet (20% of that present in the unreacted starting material)	Trichoderma reesei	cellotriose + cellobiose	-	?
cellopentaose + H2O	cellobiose is released from the reducing end by exocellulase E4 (89%), cellopentaose is labeled at the reducing end with (18)O, the products are analyzed by ionspray mass spectrometry. This value is approximate since IS-MS is unable to detect glucose under the conditions used. As a result, this value is based only on the amount of (18)O present in CTet (20% of that present in the unreacted starting material)	Thermobifida fusca	cellotriose + cellobiose	-	?

Synonyms

Synonyms	Comment	Organism
CBH I	-	Trichoderma reesei
exocellulase E4	-	Thermobifida fusca
exocellulase E6	-	Thermobifida fusca

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Release 2023.1 (January 2023)