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Literature summary for 3.2.1.176 extracted from

  • Voutilainen, S.P.; Puranen, T.; Siika-Aho, M.; Lappalainen, A.; Alapuranen, M.; Kallio, J.; Hooman, S.; Viikari, L.; Vehmaanperae, J.; Koivula, A.
    Cloning, expression, and characterization of novel thermostable family 7 cellobiohydrolases (2008), Biotechnol. Bioeng., 101, 515-528.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type Cel7A expressed in Trichoderma reesei Trichoderma reesei
wild-type Cel7A expressed in Trichoderma reesei Acremonium thermophilum
wild-type Cel7A expressed in Trichoderma reesei Thermochaetoides thermophila
wild-type Cel7A, Cel7A genetically linked to the linker and carbohydrate-binding module of Trichoderma reesei Cel7A or to the linker and carbohydrate-binding module of Chaetomium thermophilum Cel7A expressed in Trichoderma reesei Thermoascus aurantiacus
wild-type Cel7B expressed in Trichoderma reesei Acremonium thermophilum

Inhibitors

Inhibitors Comment Organism Structure
cellobiose competitive inhibition Acremonium thermophilum
cellobiose weakly inhibits, competitive inhibition Thermoascus aurantiacus
cellobiose competitive inhibition Thermochaetoides thermophila
cellobiose competitive inhibition Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
4-methylumbelliferyl-beta-D-lactoside in 50 mM sodium acetate buffer, pH 5.0, at 22°C Acremonium thermophilum
0.221
-
4-methylumbelliferyl-beta-D-lactoside in 50 mM sodium acetate buffer, pH 5.0, at 22°C Thermochaetoides thermophila
0.268
-
4-methylumbelliferyl-beta-D-lactoside in 50 mM sodium acetate buffer, pH 5.0, at 22°C Thermoascus aurantiacus
0.287
-
4-methylumbelliferyl-beta-D-lactoside in 50 mM sodium acetate buffer, pH 5.0, at 22°C Trichoderma reesei
0.52
-
2-chloro-4-nitrophenyl-beta-D-lactoside in 50 mM sodium phosphate buffer, pH 5.7, at 22°C Trichoderma reesei
0.99
-
2-chloro-4-nitrophenyl-beta-D-lactoside in 50 mM sodium phosphate buffer, pH 5.7, at 22°C Thermoascus aurantiacus
2
-
2-chloro-4-nitrophenyl-beta-D-lactoside in 50 mM sodium phosphate buffer, pH 5.7, at 22°C Thermochaetoides thermophila
2.1
-
2-chloro-4-nitrophenyl-beta-D-lactoside in 50 mM sodium phosphate buffer, pH 5.7, at 22°C Acremonium thermophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46900
-
sequence analysis Thermoascus aurantiacus
47100
-
sequence analysis Acremonium thermophilum
53700
-
sequence analysis Acremonium thermophilum
54600
-
sequence analysis Thermochaetoides thermophila

Organism

Organism UniProt Comment Textmining
Acremonium thermophilum A7WNT9
-
-
Acremonium thermophilum A7WNU0
-
-
Acremonium thermophilum ALKO4245 A7WNT9
-
-
Acremonium thermophilum ALKO4245 A7WNU0
-
-
Thermoascus aurantiacus A7WNU2
-
-
Thermoascus aurantiacus ALKO4242 A7WNU2
-
-
Thermochaetoides thermophila A7WNU1
-
-
Thermochaetoides thermophila ALKO4265 A7WNU1
-
-
Trichoderma reesei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
on affinity column and by gel filtration Trichoderma reesei
on affinity column and by gel filtration Acremonium thermophilum
on affinity column and by gel filtration Thermoascus aurantiacus
on affinity column and by gel filtration Thermochaetoides thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloro-4-nitrophenyl-beta-D-lactoside
-
Trichoderma reesei 2-chloro-4-nitrophenol + beta-D-lactose
-
?
2-chloro-4-nitrophenyl-beta-D-lactoside
-
Acremonium thermophilum 2-chloro-4-nitrophenol + beta-D-lactose
-
?
2-chloro-4-nitrophenyl-beta-D-lactoside
-
Thermoascus aurantiacus 2-chloro-4-nitrophenol + beta-D-lactose
-
?
2-chloro-4-nitrophenyl-beta-D-lactoside
-
Thermochaetoides thermophila 2-chloro-4-nitrophenol + beta-D-lactose
-
?
2-chloro-4-nitrophenyl-beta-D-lactoside
-
Acremonium thermophilum ALKO4245 2-chloro-4-nitrophenol + beta-D-lactose
-
?
2-chloro-4-nitrophenyl-beta-D-lactoside
-
Thermochaetoides thermophila ALKO4265 2-chloro-4-nitrophenol + beta-D-lactose
-
?
2-chloro-4-nitrophenyl-beta-D-lactoside
-
Thermoascus aurantiacus ALKO4242 2-chloro-4-nitrophenol + beta-D-lactose
-
?
4-methylumbelliferyl-beta-D-lactoside + H2O
-
Trichoderma reesei 4-methylumbelliferol + beta-D-lactose
-
?
4-methylumbelliferyl-beta-D-lactoside + H2O
-
Acremonium thermophilum 4-methylumbelliferol + beta-D-lactose
-
?
4-methylumbelliferyl-beta-D-lactoside + H2O
-
Thermoascus aurantiacus 4-methylumbelliferol + beta-D-lactose
-
?
4-methylumbelliferyl-beta-D-lactoside + H2O
-
Thermochaetoides thermophila 4-methylumbelliferol + beta-D-lactose
-
?
4-methylumbelliferyl-beta-D-lactoside + H2O
-
Acremonium thermophilum ALKO4245 4-methylumbelliferol + beta-D-lactose
-
?
4-methylumbelliferyl-beta-D-lactoside + H2O
-
Thermochaetoides thermophila ALKO4265 4-methylumbelliferol + beta-D-lactose
-
?
4-methylumbelliferyl-beta-D-lactoside + H2O
-
Thermoascus aurantiacus ALKO4242 4-methylumbelliferol + beta-D-lactose
-
?
avicel + H2O
-
Thermoascus aurantiacus cellobiose + D-glucose + cellotriose
-
?
avicel + H2O absorption to avicel at 4°C is 84% Thermochaetoides thermophila cellobiose + D-glucose + cellotriose
-
?
avicel + H2O absorption to avicel at 4°C is 87.5% Acremonium thermophilum cellobiose + D-glucose + cellotriose
-
?
avicel + H2O absorption to avicel at 4°C is 95.5% Trichoderma reesei cellobiose + D-glucose + cellotriose
-
?
avicel + H2O absorption to avicel at 4°C is 87.5% Acremonium thermophilum ALKO4245 cellobiose + D-glucose + cellotriose
-
?
avicel + H2O absorption to avicel at 4°C is 84% Thermochaetoides thermophila ALKO4265 cellobiose + D-glucose + cellotriose
-
?
avicel + H2O
-
Thermoascus aurantiacus ALKO4242 cellobiose + D-glucose + cellotriose
-
?
additional information no activity with 4-methylumbelliferyl-beta-D-lactoside Acremonium thermophilum ?
-
?
additional information no activity with 4-methylumbelliferyl-beta-D-lactoside Acremonium thermophilum ALKO4245 ?
-
?
phosphoric acid swollen cellulose + H2O
-
Trichoderma reesei cellobiose + D-glucose + cellotriose
-
?
phosphoric acid swollen cellulose + H2O
-
Acremonium thermophilum cellobiose + D-glucose + cellotriose
-
?
phosphoric acid swollen cellulose + H2O
-
Thermoascus aurantiacus cellobiose + D-glucose + cellotriose
-
?
phosphoric acid swollen cellulose + H2O
-
Thermochaetoides thermophila cellobiose + D-glucose + cellotriose
-
?
phosphoric acid swollen cellulose + H2O
-
Acremonium thermophilum ALKO4245 cellobiose + D-glucose + cellotriose
-
?
phosphoric acid swollen cellulose + H2O
-
Thermochaetoides thermophila ALKO4265 cellobiose + D-glucose + cellotriose
-
?
phosphoric acid swollen cellulose + H2O
-
Thermoascus aurantiacus ALKO4242 cellobiose + D-glucose + cellotriose
-
?

Synonyms

Synonyms Comment Organism
Cel7A
-
Trichoderma reesei
Cel7A
-
Acremonium thermophilum
Cel7A
-
Thermoascus aurantiacus
Cel7A
-
Thermochaetoides thermophila
Cel7B
-
Acremonium thermophilum
cellobiohydrolase
-
Trichoderma reesei
cellobiohydrolase
-
Acremonium thermophilum
cellobiohydrolase
-
Thermoascus aurantiacus
cellobiohydrolase
-
Thermochaetoides thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Trichoderma reesei
60
-
-
Acremonium thermophilum
65
-
-
Thermoascus aurantiacus
65
-
-
Thermochaetoides thermophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0283
-
2-chloro-4-nitrophenyl-beta-D-lactoside in 50 mM sodium phosphate buffer, pH 5.7, at 22°C Thermoascus aurantiacus
0.043
-
2-chloro-4-nitrophenyl-beta-D-lactoside in 50 mM sodium phosphate buffer, pH 5.7, at 22°C Trichoderma reesei
0.0467
-
2-chloro-4-nitrophenyl-beta-D-lactoside in 50 mM sodium phosphate buffer, pH 5.7, at 22°C Acremonium thermophilum
0.1883
-
4-methylumbelliferyl-beta-D-lactoside in 50 mM sodium acetate buffer, pH 5.0, at 22°C Acremonium thermophilum
0.3167
-
2-chloro-4-nitrophenyl-beta-D-lactoside in 50 mM sodium phosphate buffer, pH 5.7, at 22°C Thermochaetoides thermophila
0.325
-
4-methylumbelliferyl-beta-D-lactoside in 50 mM sodium acetate buffer, pH 5.0, at 22°C Thermoascus aurantiacus
0.4783
-
4-methylumbelliferyl-beta-D-lactoside in 50 mM sodium acetate buffer, pH 5.0, at 22°C Trichoderma reesei
1.15
-
4-methylumbelliferyl-beta-D-lactoside in 50 mM sodium acetate buffer, pH 5.0, at 22°C Thermochaetoides thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Trichoderma reesei
4
-
-
Thermochaetoides thermophila
5
-
-
Acremonium thermophilum
5
-
-
Thermoascus aurantiacus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.019
-
cellobiose
-
Trichoderma reesei
0.039
-
cellobiose
-
Thermochaetoides thermophila
0.107
-
cellobiose
-
Thermoascus aurantiacus
0.141
-
cellobiose
-
Acremonium thermophilum

pI Value

Organism Comment pI Value Maximum pI Value
Thermoascus aurantiacus
-
-
4.44
Acremonium thermophilum
-
-
4.67
Acremonium thermophilum
-
-
4.83
Thermochaetoides thermophila
-
-
5.05