Cloned (Comment) | Organism |
---|---|
wild-type Cel7A expressed in Trichoderma reesei | Trichoderma reesei |
wild-type Cel7A expressed in Trichoderma reesei | Acremonium thermophilum |
wild-type Cel7A expressed in Trichoderma reesei | Thermochaetoides thermophila |
wild-type Cel7A, Cel7A genetically linked to the linker and carbohydrate-binding module of Trichoderma reesei Cel7A or to the linker and carbohydrate-binding module of Chaetomium thermophilum Cel7A expressed in Trichoderma reesei | Thermoascus aurantiacus |
wild-type Cel7B expressed in Trichoderma reesei | Acremonium thermophilum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cellobiose | competitive inhibition | Acremonium thermophilum | |
cellobiose | weakly inhibits, competitive inhibition | Thermoascus aurantiacus | |
cellobiose | competitive inhibition | Thermochaetoides thermophila | |
cellobiose | competitive inhibition | Trichoderma reesei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
4-methylumbelliferyl-beta-D-lactoside | in 50 mM sodium acetate buffer, pH 5.0, at 22°C | Acremonium thermophilum | |
0.221 | - |
4-methylumbelliferyl-beta-D-lactoside | in 50 mM sodium acetate buffer, pH 5.0, at 22°C | Thermochaetoides thermophila | |
0.268 | - |
4-methylumbelliferyl-beta-D-lactoside | in 50 mM sodium acetate buffer, pH 5.0, at 22°C | Thermoascus aurantiacus | |
0.287 | - |
4-methylumbelliferyl-beta-D-lactoside | in 50 mM sodium acetate buffer, pH 5.0, at 22°C | Trichoderma reesei | |
0.52 | - |
2-chloro-4-nitrophenyl-beta-D-lactoside | in 50 mM sodium phosphate buffer, pH 5.7, at 22°C | Trichoderma reesei | |
0.99 | - |
2-chloro-4-nitrophenyl-beta-D-lactoside | in 50 mM sodium phosphate buffer, pH 5.7, at 22°C | Thermoascus aurantiacus | |
2 | - |
2-chloro-4-nitrophenyl-beta-D-lactoside | in 50 mM sodium phosphate buffer, pH 5.7, at 22°C | Thermochaetoides thermophila | |
2.1 | - |
2-chloro-4-nitrophenyl-beta-D-lactoside | in 50 mM sodium phosphate buffer, pH 5.7, at 22°C | Acremonium thermophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46900 | - |
sequence analysis | Thermoascus aurantiacus |
47100 | - |
sequence analysis | Acremonium thermophilum |
53700 | - |
sequence analysis | Acremonium thermophilum |
54600 | - |
sequence analysis | Thermochaetoides thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acremonium thermophilum | A7WNT9 | - |
- |
Acremonium thermophilum | A7WNU0 | - |
- |
Acremonium thermophilum ALKO4245 | A7WNT9 | - |
- |
Acremonium thermophilum ALKO4245 | A7WNU0 | - |
- |
Thermoascus aurantiacus | A7WNU2 | - |
- |
Thermoascus aurantiacus ALKO4242 | A7WNU2 | - |
- |
Thermochaetoides thermophila | A7WNU1 | - |
- |
Thermochaetoides thermophila ALKO4265 | A7WNU1 | - |
- |
Trichoderma reesei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on affinity column and by gel filtration | Trichoderma reesei |
on affinity column and by gel filtration | Acremonium thermophilum |
on affinity column and by gel filtration | Thermoascus aurantiacus |
on affinity column and by gel filtration | Thermochaetoides thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chloro-4-nitrophenyl-beta-D-lactoside | - |
Trichoderma reesei | 2-chloro-4-nitrophenol + beta-D-lactose | - |
? | |
2-chloro-4-nitrophenyl-beta-D-lactoside | - |
Acremonium thermophilum | 2-chloro-4-nitrophenol + beta-D-lactose | - |
? | |
2-chloro-4-nitrophenyl-beta-D-lactoside | - |
Thermoascus aurantiacus | 2-chloro-4-nitrophenol + beta-D-lactose | - |
? | |
2-chloro-4-nitrophenyl-beta-D-lactoside | - |
Thermochaetoides thermophila | 2-chloro-4-nitrophenol + beta-D-lactose | - |
? | |
2-chloro-4-nitrophenyl-beta-D-lactoside | - |
Acremonium thermophilum ALKO4245 | 2-chloro-4-nitrophenol + beta-D-lactose | - |
? | |
2-chloro-4-nitrophenyl-beta-D-lactoside | - |
Thermochaetoides thermophila ALKO4265 | 2-chloro-4-nitrophenol + beta-D-lactose | - |
? | |
2-chloro-4-nitrophenyl-beta-D-lactoside | - |
Thermoascus aurantiacus ALKO4242 | 2-chloro-4-nitrophenol + beta-D-lactose | - |
? | |
4-methylumbelliferyl-beta-D-lactoside + H2O | - |
Trichoderma reesei | 4-methylumbelliferol + beta-D-lactose | - |
? | |
4-methylumbelliferyl-beta-D-lactoside + H2O | - |
Acremonium thermophilum | 4-methylumbelliferol + beta-D-lactose | - |
? | |
4-methylumbelliferyl-beta-D-lactoside + H2O | - |
Thermoascus aurantiacus | 4-methylumbelliferol + beta-D-lactose | - |
? | |
4-methylumbelliferyl-beta-D-lactoside + H2O | - |
Thermochaetoides thermophila | 4-methylumbelliferol + beta-D-lactose | - |
? | |
4-methylumbelliferyl-beta-D-lactoside + H2O | - |
Acremonium thermophilum ALKO4245 | 4-methylumbelliferol + beta-D-lactose | - |
? | |
4-methylumbelliferyl-beta-D-lactoside + H2O | - |
Thermochaetoides thermophila ALKO4265 | 4-methylumbelliferol + beta-D-lactose | - |
? | |
4-methylumbelliferyl-beta-D-lactoside + H2O | - |
Thermoascus aurantiacus ALKO4242 | 4-methylumbelliferol + beta-D-lactose | - |
? | |
avicel + H2O | - |
Thermoascus aurantiacus | cellobiose + D-glucose + cellotriose | - |
? | |
avicel + H2O | absorption to avicel at 4°C is 84% | Thermochaetoides thermophila | cellobiose + D-glucose + cellotriose | - |
? | |
avicel + H2O | absorption to avicel at 4°C is 87.5% | Acremonium thermophilum | cellobiose + D-glucose + cellotriose | - |
? | |
avicel + H2O | absorption to avicel at 4°C is 95.5% | Trichoderma reesei | cellobiose + D-glucose + cellotriose | - |
? | |
avicel + H2O | absorption to avicel at 4°C is 87.5% | Acremonium thermophilum ALKO4245 | cellobiose + D-glucose + cellotriose | - |
? | |
avicel + H2O | absorption to avicel at 4°C is 84% | Thermochaetoides thermophila ALKO4265 | cellobiose + D-glucose + cellotriose | - |
? | |
avicel + H2O | - |
Thermoascus aurantiacus ALKO4242 | cellobiose + D-glucose + cellotriose | - |
? | |
additional information | no activity with 4-methylumbelliferyl-beta-D-lactoside | Acremonium thermophilum | ? | - |
? | |
additional information | no activity with 4-methylumbelliferyl-beta-D-lactoside | Acremonium thermophilum ALKO4245 | ? | - |
? | |
phosphoric acid swollen cellulose + H2O | - |
Trichoderma reesei | cellobiose + D-glucose + cellotriose | - |
? | |
phosphoric acid swollen cellulose + H2O | - |
Acremonium thermophilum | cellobiose + D-glucose + cellotriose | - |
? | |
phosphoric acid swollen cellulose + H2O | - |
Thermoascus aurantiacus | cellobiose + D-glucose + cellotriose | - |
? | |
phosphoric acid swollen cellulose + H2O | - |
Thermochaetoides thermophila | cellobiose + D-glucose + cellotriose | - |
? | |
phosphoric acid swollen cellulose + H2O | - |
Acremonium thermophilum ALKO4245 | cellobiose + D-glucose + cellotriose | - |
? | |
phosphoric acid swollen cellulose + H2O | - |
Thermochaetoides thermophila ALKO4265 | cellobiose + D-glucose + cellotriose | - |
? | |
phosphoric acid swollen cellulose + H2O | - |
Thermoascus aurantiacus ALKO4242 | cellobiose + D-glucose + cellotriose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cel7A | - |
Trichoderma reesei |
Cel7A | - |
Acremonium thermophilum |
Cel7A | - |
Thermoascus aurantiacus |
Cel7A | - |
Thermochaetoides thermophila |
Cel7B | - |
Acremonium thermophilum |
cellobiohydrolase | - |
Trichoderma reesei |
cellobiohydrolase | - |
Acremonium thermophilum |
cellobiohydrolase | - |
Thermoascus aurantiacus |
cellobiohydrolase | - |
Thermochaetoides thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Trichoderma reesei |
60 | - |
- |
Acremonium thermophilum |
65 | - |
- |
Thermoascus aurantiacus |
65 | - |
- |
Thermochaetoides thermophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0283 | - |
2-chloro-4-nitrophenyl-beta-D-lactoside | in 50 mM sodium phosphate buffer, pH 5.7, at 22°C | Thermoascus aurantiacus | |
0.043 | - |
2-chloro-4-nitrophenyl-beta-D-lactoside | in 50 mM sodium phosphate buffer, pH 5.7, at 22°C | Trichoderma reesei | |
0.0467 | - |
2-chloro-4-nitrophenyl-beta-D-lactoside | in 50 mM sodium phosphate buffer, pH 5.7, at 22°C | Acremonium thermophilum | |
0.1883 | - |
4-methylumbelliferyl-beta-D-lactoside | in 50 mM sodium acetate buffer, pH 5.0, at 22°C | Acremonium thermophilum | |
0.3167 | - |
2-chloro-4-nitrophenyl-beta-D-lactoside | in 50 mM sodium phosphate buffer, pH 5.7, at 22°C | Thermochaetoides thermophila | |
0.325 | - |
4-methylumbelliferyl-beta-D-lactoside | in 50 mM sodium acetate buffer, pH 5.0, at 22°C | Thermoascus aurantiacus | |
0.4783 | - |
4-methylumbelliferyl-beta-D-lactoside | in 50 mM sodium acetate buffer, pH 5.0, at 22°C | Trichoderma reesei | |
1.15 | - |
4-methylumbelliferyl-beta-D-lactoside | in 50 mM sodium acetate buffer, pH 5.0, at 22°C | Thermochaetoides thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Trichoderma reesei |
4 | - |
- |
Thermochaetoides thermophila |
5 | - |
- |
Acremonium thermophilum |
5 | - |
- |
Thermoascus aurantiacus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
cellobiose | - |
Trichoderma reesei | |
0.039 | - |
cellobiose | - |
Thermochaetoides thermophila | |
0.107 | - |
cellobiose | - |
Thermoascus aurantiacus | |
0.141 | - |
cellobiose | - |
Acremonium thermophilum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermoascus aurantiacus | - |
- |
4.44 |
Acremonium thermophilum | - |
- |
4.67 |
Acremonium thermophilum | - |
- |
4.83 |
Thermochaetoides thermophila | - |
- |
5.05 |