Literature summary for 3.2.1.176 extracted from

Ubhayasekera, W.; Munoz, I.G.; Vasella, A.; Stahlberg, J.; Mowbray, S.L.
Structures of Phanerochaete chrysosporium Cel7D in complex with product and inhibitors (2005), FEBS J., 272, 1952-1964.

Search for more literature for 3.2.1.176

Crystallization (Commentary)

Crystallization (Comment)	Organism
deglycosylated Cel7D complexed with cellobiose, or with inhibitors cellobioimidazole, lactose, or methyl (4S)-beta-cellobiosyl-4-thio-beta-cellobioside, hanging drop vapour diffusion method, 18 mg/ml protein in 10 mM Tris-HCl, pH 7.0, 5 mM CaCl2, 15-22.5% PEG 5000, and 12% glycerol, soaking of crystals in ligand solution containing 10 mM ligand, X-ray diffraction structure determination and analysis at -173°C and 1.7-1.8 A resolution	Phanerodontia chrysosporium

Crystallization (Comment)

Organism

deglycosylated Cel7D complexed with cellobiose, or with inhibitors cellobioimidazole, lactose, or methyl (4S)-beta-cellobiosyl-4-thio-beta-cellobioside, hanging drop vapour diffusion method, 18 mg/ml protein in 10 mM Tris-HCl, pH 7.0, 5 mM CaCl2, 15-22.5% PEG 5000, and 12% glycerol, soaking of crystals in ligand solution containing 10 mM ligand, X-ray diffraction structure determination and analysis at -173°C and 1.7-1.8 A resolution

Phanerodontia chrysosporium

Protein Variants

Protein Variants	Comment	Organism
E217Q	inactive mutant	Phanerodontia chrysosporium

Inhibitors

Inhibitors	Comment	Organism
Ca2+	-	Phanerodontia chrysosporium
cellobioimidazole	i.e. (5R,6R,7S,8S)-6-(beta-D-glucopyranosyloxy)-5,6,7,8-tetrahydro-5-[(hydroxy)methyl]imidazol[1,2a] pyridine-7,8-diol, the disaccharide binds in the glycosyl-binding subsites +1 and +2 close to the exit of the cellulose-binding tunnel/cleft of Cel7D, binding structure analysis	Phanerodontia chrysosporium
lactose	the disaccharide binds in the glycosyl-binding subsites +1 and +2 close to the exit of the cellulose-binding tunnel/cleft of Cel7D, binding structure analysis	Phanerodontia chrysosporium
methyl (4S)-beta-cellobiosyl-4-thio-beta-cellobioside	a thio-linked substrate analogue, the disaccharide binds in the glycosyl-binding subsites +1 and +2 close to the exit of the cellulose-binding tunnel/cleft of Cel7D, binding structure analysis	Phanerodontia chrysosporium
Tris	-	Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Phanerodontia chrysosporium

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	secretion of Cel7D to the medium	Phanerodontia chrysosporium	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cellulose + H2O	Phanerodontia chrysosporium	-	cellobiose + ?	-	?

Organism

Organism	UniProt	Comment	Textmining
Phanerodontia chrysosporium	Q7LIJ0	White Rot fungus, Cel7D is the major cellulase	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	Cel7D	Phanerodontia chrysosporium

Reaction

Reaction	Comment	Organism	Reaction ID
2 cellohexaose + 2 H2O = 2 cellotriose + cellotetraose + cellobiose	substrate binding structure analysis of Cel7D	Phanerodontia chrysosporium	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cellulose + H2O	-	Phanerodontia chrysosporium	cellobiose + ?	-	?
additional information	substrate binding structure analysis of Cel7D	Phanerodontia chrysosporium	?	-	?

Synonyms

Synonyms	Comment	Organism
Cel7D	-	Phanerodontia chrysosporium
More	the enzyme Cel7D belongs to the glycoside hydrolase family 7	Phanerodontia chrysosporium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Phanerodontia chrysosporium