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Literature summary for 3.2.1.176 extracted from

  • Gusakov, A.V.; Sinitsyn, A.P.; Salanovich, T.N.; Bukhtojarov, F.E.; Markov, A.V.; Ustinov, B.B.; van Zeijl, C.; Punt, P.; Burlingame, R.
    Purification, cloning and characterisation of two forms of thermostable and highly active cellobiohydrolase I (Cel7A) produced by the industrial strain of Chrysosporium lucknowense (2005), Enzyme Microb. Technol., 36, 57-69.
No PubMed abstract available
Search for more literature for 3.2.1.176

Cloned(Commentary)

Cloned (Comment) Organism
gene cbh1, DNA and amino acid sequence determination Chrysosporium lucknowense

Protein Variants

Protein Variants Comment Organism
additional information isolation of the truncated catalytic core enzyme form 2 lacking the cellulose binding module and the major part of glycosylated linker from a mutant strain Chrysosporium lucknowense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 x * 65000, full-length enzyme form 1, SDS-PAGE, x * 52000, truncated catalytic core enzyme form 2, SDS-PAGE Chrysosporium lucknowense
65000
-
 x * 65000, full-length enzyme form 1, SDS-PAGE, x * 52000, truncated catalytic core enzyme form 2, SDS-PAGE Chrysosporium lucknowense

Organism

Organism UniProt Comment Textmining
Chrysosporium lucknowense
-
 gene cbh1, wild-type and mutant industrial strain, full-length and mutant enzyme forms
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O
-
 Chrysosporium lucknowense ?
-
 ?
cellulose + H2O from cotton Chrysosporium lucknowense beta-D-cellobiose
-
 ?
additional information the full-length and mutant enzyme forms show similar substrate specificities Chrysosporium lucknowense ?
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, full-length enzyme form 1, SDS-PAGE, x * 52000, truncated catalytic core enzyme form 2, SDS-PAGE Chrysosporium lucknowense
More peptide sequencing of Cel7A and CBH I Chrysosporium lucknowense

Synonyms

Synonyms Comment Organism
CBH I
-
 Chrysosporium lucknowense
Cel7A
-
 Chrysosporium lucknowense
cellobiohydrolase I
-
 Chrysosporium lucknowense
More the enzyme Cel7A probably belongs to the cellobiohydrolase family 7 Chrysosporium lucknowense

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 the full-length enzyme form is more thermostable than the truncated mutant enzyme form Chrysosporium lucknowense
50
-
 24 h, stable, full-length and mutant enzyme forms Chrysosporium lucknowense
60
-
 7 h, 90% remaining activity of the full-length enzyme form Chrysosporium lucknowense
65
-
 3 h, 50% remaining activity of the full-length enzyme form Chrysosporium lucknowense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5 full-length and mutant enzyme forms Chrysosporium lucknowense

pI Value

Organism Comment pI Value Maximum pI Value
Chrysosporium lucknowense full-length and mutant enzyme forms
-
 4.5