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Literature summary for 3.2.1.176 extracted from

  • Zverlov, V.V.; Velikodvorskaya, G.A.; Schwarz, W.H.
    A newly described cellulosomal cellobiohydrolase, CelO, from Clostridium thermocellum: investigation of the exo-mode of hydrolysis, and binding capacity to crystalline cellulose (2002), Microbiology, 148, 247-255.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
native enzyme and truncated versions Acetivibrio thermocellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75240
-
calculated from nucleic acid sequence Acetivibrio thermocellus
75244
-
x * 75244, calculated from sequence Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-
Acetivibrio thermocellus Q9L3J2
-
-
Acetivibrio thermocellus F7
-
-
-
Acetivibrio thermocellus F7 Q9L3J2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme and truncated versions, homogeneity Acetivibrio thermocellus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1
-
with phosphoric acid swollen cellulose as substrate Acetivibrio thermocellus
7.5
-
with carboxymethylcellulose as substrate Acetivibrio thermocellus
340
-
with barley beta-glucan as substrate Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellotetraoside + H2O enzyme activity starts from the reducing end in a processive mode after making random cuts Acetivibrio thermocellus ?
-
?
4-nitrophenyl beta-D-cellotetraoside + H2O enzyme activity starts from the reducing end in a processive mode after making random cuts Acetivibrio thermocellus F7 ?
-
?
4-nitrophenyl cellotrioside + H2O
-
Acetivibrio thermocellus cellobiose + 4-nitrophenyl beta-D-glucoside
-
?
4-nitrophenyl cellotrioside + H2O
-
Acetivibrio thermocellus F7 cellobiose + 4-nitrophenyl beta-D-glucoside
-
?
beta-glucan + H2O from barley Acetivibrio thermocellus ?
-
?
beta-glucan + H2O from barley Acetivibrio thermocellus F7 ?
-
?
carboxymethyl cellulose + H2O
-
Acetivibrio thermocellus ?
-
?
carboxymethyl cellulose + H2O
-
Acetivibrio thermocellus F7 ?
-
?
cellodextrin + H2O
-
Acetivibrio thermocellus ?
-
?
cellodextrin + H2O
-
Acetivibrio thermocellus F7 ?
-
?
cellooligosaccharide + H2O
-
Acetivibrio thermocellus cellobiose
-
?
cellooligosaccharide + H2O
-
Acetivibrio thermocellus F7 cellobiose
-
?
cellotetraose + H2O
-
Acetivibrio thermocellus 2 cellobiose
-
?
cellotetraose + H2O
-
Acetivibrio thermocellus F7 2 cellobiose
-
?
cellulose + H2O
-
Acetivibrio thermocellus cellobiose + ?
-
?
cellulose + H2O the recombinant gene product is active on cellodextrins, barley beta-glucan, carboxymethylcellulose and insoluble cellulose. Cellobiose is the only product released from amorphic and crystalline cellulose, cellotetraose and higher cellooligosaccharides. The cleavage pattern of p-nitrophenyl beta-D-cellotetraoside, blockage of the hydrolysis of NaBH4-reduced cellopentaose and the reduction in substrate viscosity suggests activity from the reducing end in a processive mode after making random cuts. Binding to insoluble, i.e. amorphous, and crystalline cellulose is mediated by the carbohydrate-binding module CBM3b, with a preference for the crystalline substrate Acetivibrio thermocellus cellobiose + ?
-
?
cellulose + H2O the recombinant gene product is active on cellodextrins, barley beta-glucan, carboxymethylcellulose and insoluble cellulose. Cellobiose is the only product released from amorphic and crystalline cellulose, cellotetraose and higher cellooligosaccharides. The cleavage pattern of p-nitrophenyl beta-D-cellotetraoside, blockage of the hydrolysis of NaBH4-reduced cellopentaose and the reduction in substrate viscosity suggests activity from the reducing end in a processive mode after making random cuts. Binding to insoluble, i.e. amorphous, and crystalline cellulose is mediated by the carbohydrate-binding module CBM3b, with a preference for the crystalline substrate Acetivibrio thermocellus F7 cellobiose + ?
-
?
additional information rCelO completely lacks chromogenic activity on the p-nitrophenyl-cellodextrins from p-nitrophenyl glucoside to 4-nitrophenyl cellopentaoside Acetivibrio thermocellus ?
-
?
additional information rCelO completely lacks chromogenic activity on the p-nitrophenyl-cellodextrins from p-nitrophenyl glucoside to 4-nitrophenyl cellopentaoside Acetivibrio thermocellus F7 ?
-
?
phosphoric acid swollen cellulose + H2O
-
Acetivibrio thermocellus ?
-
?

Subunits

Subunits Comment Organism
? x * 75244, calculated from sequence Acetivibrio thermocellus

Synonyms

Synonyms Comment Organism
CelO
-
Acetivibrio thermocellus
Ct-Cel5F
-
Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
-
Acetivibrio thermocellus

General Information

General Information Comment Organism
physiological function the enzyme was a component of the cellulosome complex Acetivibrio thermocellus