Cloned (Comment) | Organism |
---|---|
native enzyme and truncated versions | Acetivibrio thermocellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75240 | - |
calculated from nucleic acid sequence | Acetivibrio thermocellus |
75244 | - |
x * 75244, calculated from sequence | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
- |
- |
Acetivibrio thermocellus | Q9L3J2 | - |
- |
Acetivibrio thermocellus F7 | - |
- |
- |
Acetivibrio thermocellus F7 | Q9L3J2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme and truncated versions, homogeneity | Acetivibrio thermocellus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.1 | - |
with phosphoric acid swollen cellulose as substrate | Acetivibrio thermocellus |
7.5 | - |
with carboxymethylcellulose as substrate | Acetivibrio thermocellus |
340 | - |
with barley beta-glucan as substrate | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-cellotetraoside + H2O | enzyme activity starts from the reducing end in a processive mode after making random cuts | Acetivibrio thermocellus | ? | - |
? | |
4-nitrophenyl beta-D-cellotetraoside + H2O | enzyme activity starts from the reducing end in a processive mode after making random cuts | Acetivibrio thermocellus F7 | ? | - |
? | |
4-nitrophenyl cellotrioside + H2O | - |
Acetivibrio thermocellus | cellobiose + 4-nitrophenyl beta-D-glucoside | - |
? | |
4-nitrophenyl cellotrioside + H2O | - |
Acetivibrio thermocellus F7 | cellobiose + 4-nitrophenyl beta-D-glucoside | - |
? | |
beta-glucan + H2O | from barley | Acetivibrio thermocellus | ? | - |
? | |
beta-glucan + H2O | from barley | Acetivibrio thermocellus F7 | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Acetivibrio thermocellus F7 | ? | - |
? | |
cellodextrin + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
cellodextrin + H2O | - |
Acetivibrio thermocellus F7 | ? | - |
? | |
cellooligosaccharide + H2O | - |
Acetivibrio thermocellus | cellobiose | - |
? | |
cellooligosaccharide + H2O | - |
Acetivibrio thermocellus F7 | cellobiose | - |
? | |
cellotetraose + H2O | - |
Acetivibrio thermocellus | 2 cellobiose | - |
? | |
cellotetraose + H2O | - |
Acetivibrio thermocellus F7 | 2 cellobiose | - |
? | |
cellulose + H2O | - |
Acetivibrio thermocellus | cellobiose + ? | - |
? | |
cellulose + H2O | the recombinant gene product is active on cellodextrins, barley beta-glucan, carboxymethylcellulose and insoluble cellulose. Cellobiose is the only product released from amorphic and crystalline cellulose, cellotetraose and higher cellooligosaccharides. The cleavage pattern of p-nitrophenyl beta-D-cellotetraoside, blockage of the hydrolysis of NaBH4-reduced cellopentaose and the reduction in substrate viscosity suggests activity from the reducing end in a processive mode after making random cuts. Binding to insoluble, i.e. amorphous, and crystalline cellulose is mediated by the carbohydrate-binding module CBM3b, with a preference for the crystalline substrate | Acetivibrio thermocellus | cellobiose + ? | - |
? | |
cellulose + H2O | the recombinant gene product is active on cellodextrins, barley beta-glucan, carboxymethylcellulose and insoluble cellulose. Cellobiose is the only product released from amorphic and crystalline cellulose, cellotetraose and higher cellooligosaccharides. The cleavage pattern of p-nitrophenyl beta-D-cellotetraoside, blockage of the hydrolysis of NaBH4-reduced cellopentaose and the reduction in substrate viscosity suggests activity from the reducing end in a processive mode after making random cuts. Binding to insoluble, i.e. amorphous, and crystalline cellulose is mediated by the carbohydrate-binding module CBM3b, with a preference for the crystalline substrate | Acetivibrio thermocellus F7 | cellobiose + ? | - |
? | |
additional information | rCelO completely lacks chromogenic activity on the p-nitrophenyl-cellodextrins from p-nitrophenyl glucoside to 4-nitrophenyl cellopentaoside | Acetivibrio thermocellus | ? | - |
? | |
additional information | rCelO completely lacks chromogenic activity on the p-nitrophenyl-cellodextrins from p-nitrophenyl glucoside to 4-nitrophenyl cellopentaoside | Acetivibrio thermocellus F7 | ? | - |
? | |
phosphoric acid swollen cellulose + H2O | - |
Acetivibrio thermocellus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 75244, calculated from sequence | Acetivibrio thermocellus |
Synonyms | Comment | Organism |
---|---|---|
CelO | - |
Acetivibrio thermocellus |
Ct-Cel5F | - |
Acetivibrio thermocellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Acetivibrio thermocellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
- |
Acetivibrio thermocellus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme was a component of the cellulosome complex | Acetivibrio thermocellus |