Protein Variants | Comment | Organism |
---|---|---|
E217Q | stability similar to wild-type enzyme, catalytically inactive | Trichoderma reesei |
E223S/A224H/L225V/T226A/D262G | mutant has a more alkaline pH optimum, mutation destabilizes the protein fold at both acidic and alkaline pH | Trichoderma reesei |
General Stability | Organism |
---|---|
cellotetraose stabilizes the protein fold | Trichoderma reesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma reesei | - |
- |
- |
Trichoderma reesei ALKO2877 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutants | Trichoderma reesei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-beta-D-lactoside + H2O | - |
Trichoderma reesei | ? | - |
? | |
4-methylumbelliferyl-beta-D-lactoside + H2O | - |
Trichoderma reesei ALKO2877 | ? | - |
? |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 5.6 | optimal stability, gradual decrease in stability at more alkaline pH values | Trichoderma reesei |