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Literature summary for 3.2.1.176 extracted from
- The relationship between thermal stability and pH optimum studied with wild-type and mutant Trichoderma reesei cellobiohydrolase Cel7A (2003), Eur. J. Biochem., 270, 841-848.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E217Q | stability similar to wild-type enzyme, catalytically inactive | Trichoderma reesei |
| E223S/A224H/L225V/T226A/D262G | mutant has a more alkaline pH optimum, mutation destabilizes the protein fold at both acidic and alkaline pH | Trichoderma reesei |
General Stability
| General Stability | Organism |
|---|---|
| cellotetraose stabilizes the protein fold | Trichoderma reesei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma reesei | - |
- |
- |
| Trichoderma reesei ALKO2877 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| wild-type and mutants | Trichoderma reesei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-beta-D-lactoside + H2O | - |
Trichoderma reesei | ? | - |
? |  |
| 4-methylumbelliferyl-beta-D-lactoside + H2O | - |
Trichoderma reesei ALKO2877 | ? | - |
? | |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 5.6 | optimal stability, gradual decrease in stability at more alkaline pH values | Trichoderma reesei |
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Release 2023.1 (January 2023)
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