Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.176 extracted from

  • Becker, D.; Braet, C.; Brumer, H.3rd.; Claeyssens, M.; Divne, C.; Fagerstrom, B.R.; Harris, M.; Jones, T.A.; Kleywegt, G.J.; Koivula, A.; Mahdi, S.; Piens, K.; Sinnott, M.L.; Stahlberg, J.; Teeri, T.T.; Underwood, M.; Wohlfahrt, G.
    Engineering of a glycosidase family 7 cellobiohydrolase to more alkaline pH optimum: the pH behaviour of Trichoderma reesei Cel7A and its E223S/A224H/L225V/T226A/D262G mutant (2001), Biochem. J., 356, 19-30.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.176

Cloned(Commentary)

Cloned (Comment) Organism
-
 Trichoderma reesei

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method Trichoderma reesei

Protein Variants

Protein Variants Comment Organism
E223S/A224H/L225V/T226A/D262G mutant has a more alkaline pH optimum Trichoderma reesei

Inhibitors

Inhibitors Comment Organism Structure
cellobiose competitive with wild-type enzyme, mixed inhibition with mutant enzyme Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11
-
 3,4-dinitrophenyl-cellobioside 25°C, pH 7, wild-type Trichoderma reesei
14
-
 3,4-dinitrophenyl-cellobioside 25°C, pH 5, wild-type Trichoderma reesei
48
-
 3,4-dinitrophenyl-cellobioside 25°C, pH 5, E223S/A224H/L225V/T226A/D262G mutant Trichoderma reesei
161
-
 3,4-dinitrophenyl-cellobioside 25°C, pH 7, E223S/A224H/L225V/T226A/D262G mutant Trichoderma reesei
380
-
 3,4-dinitrophenyl-lactoside 25°C, pH 5, wild-type Trichoderma reesei
543
-
 3,4-dinitrophenyl-lactoside 25°C, pH 5, E223S/A224H/L225V/T226A/D262G mutant Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Trichoderma reesei P62694
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloro-4-nitrophenyl lactoside + H2O
-
 Trichoderma reesei 2-chloro-4-nitrophenol + ?
-
 ?
3,4-dinitrophenyl lactoside + H2O
-
 Trichoderma reesei 3,4-dinitrophenol + ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 enzyme retains full activity between pH 4 and 6, but is rapidly inactivated at lower pH values Trichoderma reesei

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 7 enzyme is stable at pH 3-7 at 37°C, but loses 90% of activity at pH 2 after 20 min Trichoderma reesei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.02
-
 cellobiose 25°C, wild-type enzyme Trichoderma reesei
0.755
-
 cellobiose 25°C, E223S/A224H/L225V/T226A/D262G mutant Trichoderma reesei