Cloned (Comment) | Organism |
---|---|
transient expression of HA-tagged wild-type enzyme and mutant E426Q in protoplasts, expression of GFP-tagged enzyme in vacuoles | Arabidopsis thaliana |
transient expression of HA-tagged wild-type enzyme BG1 in protoplasts | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
E426Q | site-directed mutagenesis the enzyme is not induced by Glc-conjugated abscisic acid and shows reduced activity compared to the wild-type enzyme | Arabidopsis thaliana |
additional information | overexpression of BG2 rescues the bg1 mutant phenotype, as observed for the overexpression of NCED3 in bg1 mutants. NaCl stress-sensitive root growth of bg2-1 and bg2-2 mutants | Arabidopsis thaliana |
additional information | overexpression of NCED3 in bg1 mutants | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
vacuole | BG2 localizes to the central vacuole. The N-terminal region of enzyme BG2 is responsible for the vacuolar targeting. The majority of BG2 localizes to the vacuole | Arabidopsis thaliana | 5773 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
98000 | - |
x * 98000, GFP-tagged enzyme, SDS-PAGE | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana | Q9SE50 | - |
- |
Arabidopsis thaliana Col-0 | - |
- |
- |
Arabidopsis thaliana Col-0 | Q9SE50 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GFP-tagged enzyme from vacuoles by ultracentrifugation | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Glc-conjugated abscisic acid + H2O | in vitro activity of isozyme BG2 | Arabidopsis thaliana | abscisic acid + D-glucose | - |
? | |
Glc-conjugated abscisic acid + H2O | in vitro activity of isozyme BG2 | Arabidopsis thaliana Col-0 | abscisic acid + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 98000, GFP-tagged enzyme, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
beta-glucosidase homolog 1 | - |
Arabidopsis thaliana |
beta-glucosidase homolog 2 | - |
Arabidopsis thaliana |
BG1 | - |
Arabidopsis thaliana |
BG2 | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | the recombinant enzyme is induced by Glc-conjugated abscisic acid | up |
General Information | Comment | Organism |
---|---|---|
malfunction | multiple Arabidopsis thaliana bg2 alleles with a T-DNA insertion in BG2 are more sensitive to dehydration and NaCl stress compared to the wild-type enzyme, whereas BG2 overexpression results in enhanced resistance to dehydration and NaCl stress | Arabidopsis thaliana |
physiological function | in addition to the de novo biosynthesis, abscisic acid is produced in multiple organelles by organelle-specific beta-glucosidases in response to abiotic stresses. BG2 increases abscisic acid levels in protoplasts upon application of exogenous Glc-conjugated abscisic acid | Arabidopsis thaliana |
physiological function | in Arabidopsis thaliana, abscisic acid levels are increased both through de novo biosynthesis and via beta-glucosidase homolog 1-mediated hydrolysis of Glc-conjugated abscisic acid. In addition to the de novo biosynthesis, abscisic acid is produced in multiple organelles by organelle-specific beta-glucosidases in response to abiotic stresses | Arabidopsis thaliana |