Literature summary for 3.2.1.175 extracted from

Dietz, K.J.; Sauter, A.; Wichert, K.; Messdaghi, D.; Hartung, W.
Extracellular beta-glucosidase activity in barley involved in the hydrolysis of ABA glucose conjugate in leaves (2000), J. Exp. Bot., 51, 937-944.

Search for more literature for 3.2.1.175

Inhibitors

Inhibitors	Comment	Organism	Structure
beta-D-glucopyranosyl abscisate	inhibits activity with p-nitrophenol-beta-D-glucopyranoside as substrate	Hordeum vulgare

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.8	-	p-nitrophenyl-beta-D-glucopyranoside	pH 4.8, 37°C	Hordeum vulgare

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
apoplast	-	Hordeum vulgare	48046	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
beta-D-glucopyranosyl abscisate + H2O	Hordeum vulgare	the enzyme is involved in root/shoot signalling in plants	beta-D-glucopyranose + abscisate	-	?

Organism

Organism	UniProt	Comment	Textmining
Hordeum vulgare	-	cv. Gerbel	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	isolated from primary leaf 8 and 15 days after sowing. The activity of beta-D-glucosidase declines with leaf age	Hordeum vulgare	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-D-glucopyranosyl abscisate + H2O	the enzyme is involved in root/shoot signalling in plants	Hordeum vulgare	beta-D-glucopyranose + abscisate	-	?
beta-D-glucopyranosyl abscisate + H2O	-	Hordeum vulgare	beta-D-glucose + abscisate	-	?
p-nitrophenyl-beta-D-glucopyranoside + H2O	-	Hordeum vulgare	beta-D-glucopyranose + p-nitrophenol	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Hordeum vulgare

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.8	-	assay at	Hordeum vulgare
5.5	-	-	Hordeum vulgare

pH Range

pH Minimum	pH Maximum	Comment	Organism
4.5	6.5	pH 4.5: about 50% of maximal activity, pH 6.5: about 40% of maximal activity	Hordeum vulgare

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.4	-	beta-D-glucopyranosyl abscisate	pH 4.8, 37°C	Hordeum vulgare

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Release 2023.1 (January 2023)