Protein Variants | Comment | Organism |
---|---|---|
DELTAC105 | a deletion mutant that lacks 105 residues at the C terminus. NO activity | Arabidopsis thaliana |
E207Q | the amount of abscisate released by the mutant enzyme is reduced to 25% of that of the wild-type value, indicating that glutamic acid at position 207 is important for hydrolytic activity | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | abscisate generated by AtBG1 in the endoplasmic reticulum is secreted from cells into the apoplastic space of the leaf where it may play a role in initiation of ABA signaling | Arabidopsis thaliana | 5783 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
10 * 60000, main peak of AtBG1 in dehydration-stressed plants. Dehydration rapidly induces polymerization of AtBG1, resulting in a 4fold increase in enzymatic activity. Diurnal increases in abscisate levels are attributable to polymerization-mediated AtBG1 activation | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucopyranosyl abscisate + H2O | Arabidopsis thaliana | abscisic acid is a phytohormone critical for plant growth. Dehydration rapidly induces polymerization of the beta-D-glucopyranosyl abscisate hydrolyzing enzyme AtBG1, resulting in a 4fold increase in enzymatic activity. Diurnal increases in abscisate levels are attributable to polymerization-mediated AtBG1 activation. Activation of inactive abscisate pools by polymerized AtBG1 is a mechanism by which plants rapidly adjust abscisate levels and respond to changing environmental cues. Assembly of AtBG1 into higher molecular weight forms upon dehydration stress occurs at a mild condition such as exposure to 30% relative humidity for 30 min, indicating that the response is very sensitive to dehydration stress | beta-D-glucopyranose + abscisate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SE50 | ecotype Columbia | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Arabidopsis thaliana |
additional information | dehydration rapidly induces polymerization of the beta-D-glucopyranosyl abscisate hydrolyzing enzyme AtBG1, resulting in a 4fold increase in enzymatic activity. Assembly of AtBG1 into higher molecular weight forms upon dehydration stress occurs at a mild condition such as exposure to 30% relative humidity for 30 min, indicating that the response is very sensitive to dehydration stress | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | hydathodes of rosette and cauline leaves | Arabidopsis thaliana | - |
seed | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucopyranosyl abscisate + H2O | abscisic acid is a phytohormone critical for plant growth. Dehydration rapidly induces polymerization of the beta-D-glucopyranosyl abscisate hydrolyzing enzyme AtBG1, resulting in a 4fold increase in enzymatic activity. Diurnal increases in abscisate levels are attributable to polymerization-mediated AtBG1 activation. Activation of inactive abscisate pools by polymerized AtBG1 is a mechanism by which plants rapidly adjust abscisate levels and respond to changing environmental cues. Assembly of AtBG1 into higher molecular weight forms upon dehydration stress occurs at a mild condition such as exposure to 30% relative humidity for 30 min, indicating that the response is very sensitive to dehydration stress | Arabidopsis thaliana | beta-D-glucopyranose + abscisate | - |
? | |
beta-D-glucopyranosyl abscisate + H2O | the enzyme is specific for beta-D-glucopyranosyl abscisate | Arabidopsis thaliana | beta-D-glucopyranose + abscisate | - |
? | |
additional information | the enzyme does not hydrolyze beta-D-glucopyranosyl zeatin | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 60000, main peak of AtBG1 in dehydration-stressed plants. Dehydration rapidly induces polymerization of AtBG1, resulting in a 4fold increase in enzymatic activity. Diurnal increases in abscisate levels are attributable to polymerization-mediated AtBG1 activation | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
ABA-GE hydrolyzing activity | - |
Arabidopsis thaliana |
AtBG1 | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | loss of AtBG1 causes defective stomatal movement, early germination, abiotic stress-sensitive phenotypes, and lower abscisate levels, whereas plants with ectopic AtBG1 accumulate higher abscisate levels and display enhanced tolerance to abiotic stress | Arabidopsis thaliana |
metabolism | abscisic acid is a phytohormone critical for plant growth. Dehydration rapidly induces polymerization of the beta-D-glucopyranosyl abscisate hydrolyzing enzyme AtBG1, resulting in a 4fold increase in enzymatic activity. Diurnal increases in abscisate levels are attributable to polymerization-mediated AtBG1 activation. Activation of inactive abscisate pools by polymerized AtBG1 is a mechanism by which plants rapidly adjust abscisate levels and respond to changing environmental cues | Arabidopsis thaliana |