Cloned (Comment) | Organism |
---|---|
sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Scophthalmus maximus |
Protein Variants | Comment | Organism |
---|---|---|
E50A/D67A | site-directed mutagenesis, mutation of catalytic residues, the mutant shows drastically reduced bacteriolytic activity compared to the wild-type enzyme | Scophthalmus maximus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Scophthalmus maximus | 5737 | - |
extracellular | the enzyme contains an N-terminal signal peptide sequence (residues 1-15) | Scophthalmus maximus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15700 | - |
x * 15700, recombinant His-tagged enzyme, SDS-PAGE | Scophthalmus maximus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Scophthalmus maximus | B3VHW3 | i.e. Pleuronectes maximus | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by ncikel affinity chromatography | Scophthalmus maximus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood | - |
Scophthalmus maximus | - |
brain | - |
Scophthalmus maximus | - |
gill | - |
Scophthalmus maximus | - |
head kidney | low expression | Scophthalmus maximus | - |
heart | - |
Scophthalmus maximus | - |
liver | high expression | Scophthalmus maximus | - |
additional information | enzyme expression level in increasing order in head kidney, gill, heart, muscle, brain, spleen, blood, and liver | Scophthalmus maximus | - |
muscle | - |
Scophthalmus maximus | - |
spleen | - |
Scophthalmus maximus | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 15700, recombinant His-tagged enzyme, SDS-PAGE | Scophthalmus maximus |
More | the enzyme contains an N-terminal signal peptide sequence (residues 1-15) followed by a c-type lysozyme/alpha-lactalbumin domain (residues 16-142). Residues C21 and C141, C45 and C129, C79 and C94, and C90 and 108 are predicted to form disulfide bonds | Scophthalmus maximus |
Synonyms | Comment | Organism |
---|---|---|
c-type lysozyme | - |
Scophthalmus maximus |
chicken-type lysozyme | - |
Scophthalmus maximus |
SmLysC | - |
Scophthalmus maximus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Scophthalmus maximus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Scophthalmus maximus |
General Information | Comment | Organism |
---|---|---|
additional information | the conserved residues E50 and D67 form the putative catalytic site | Scophthalmus maximus |
physiological function | lysozyme is a key component of the innate immune system and plays an important role in antibacterial infection, the c-type lysozyme participate in innate immune defense against extracellular bacterial pathogens, in particular those of Gram-positive nature, e.g. Edwardsiella tarda TX1, Pseudomonas fluorescens TSS, and Vibrio anguillarum C312. The enzyme is able to inhibit the growth of several fish bacterial pathogens in a manner that depended on the dose of the protein | Scophthalmus maximus |