Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.17 extracted from

  • Yu, L.P.; Sun, B.G.; Li, J.; Sun, L.
    Characterization of a c-type lysozyme of Scophthalmus maximus: expression, activity, and antibacterial effect (2013), Fish Shellfish Immunol., 34, 46-54.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Scophthalmus maximus

Protein Variants

Protein Variants Comment Organism
E50A/D67A site-directed mutagenesis, mutation of catalytic residues, the mutant shows drastically reduced bacteriolytic activity compared to the wild-type enzyme Scophthalmus maximus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Scophthalmus maximus 5737
-
extracellular the enzyme contains an N-terminal signal peptide sequence (residues 1-15) Scophthalmus maximus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15700
-
x * 15700, recombinant His-tagged enzyme, SDS-PAGE Scophthalmus maximus

Organism

Organism UniProt Comment Textmining
Scophthalmus maximus B3VHW3 i.e. Pleuronectes maximus
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by ncikel affinity chromatography Scophthalmus maximus

Source Tissue

Source Tissue Comment Organism Textmining
blood
-
Scophthalmus maximus
-
brain
-
Scophthalmus maximus
-
gill
-
Scophthalmus maximus
-
head kidney low expression Scophthalmus maximus
-
heart
-
Scophthalmus maximus
-
liver high expression Scophthalmus maximus
-
additional information enzyme expression level in increasing order in head kidney, gill, heart, muscle, brain, spleen, blood, and liver Scophthalmus maximus
-
muscle
-
Scophthalmus maximus
-
spleen
-
Scophthalmus maximus
-

Subunits

Subunits Comment Organism
? x * 15700, recombinant His-tagged enzyme, SDS-PAGE Scophthalmus maximus
More the enzyme contains an N-terminal signal peptide sequence (residues 1-15) followed by a c-type lysozyme/alpha-lactalbumin domain (residues 16-142). Residues C21 and C141, C45 and C129, C79 and C94, and C90 and 108 are predicted to form disulfide bonds Scophthalmus maximus

Synonyms

Synonyms Comment Organism
c-type lysozyme
-
Scophthalmus maximus
chicken-type lysozyme
-
Scophthalmus maximus
SmLysC
-
Scophthalmus maximus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Scophthalmus maximus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Scophthalmus maximus

General Information

General Information Comment Organism
additional information the conserved residues E50 and D67 form the putative catalytic site Scophthalmus maximus
physiological function lysozyme is a key component of the innate immune system and plays an important role in antibacterial infection, the c-type lysozyme participate in innate immune defense against extracellular bacterial pathogens, in particular those of Gram-positive nature, e.g. Edwardsiella tarda TX1, Pseudomonas fluorescens TSS, and Vibrio anguillarum C312. The enzyme is able to inhibit the growth of several fish bacterial pathogens in a manner that depended on the dose of the protein Scophthalmus maximus