Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, expression of enzyme mutants in Pichia pastoris, subcloning in Escherichia coli strain JM109 | Alopochen aegyptiaca |
sequence comparisons, phylogenetic tree | Aix sponsa |
Protein Variants | Comment | Organism |
---|---|---|
F34Y | site-directed mutagenesis at the substrate subsite E, the mutant shows a slightly altered product pattern compared to the wild-type enzyme | Alopochen aegyptiaca |
F34Y/N37G | site-directed mutagenesis at the substrate subsites E/F, the mutant shows a slightly altered product pattern compared to the wild-type enzyme | Alopochen aegyptiaca |
F34Y/N37G/G71R | site-directed mutagenesis, the mutant shows altered time course data compared to the wild-type enzyme | Alopochen aegyptiaca |
G71R | site-directed mutagenesis, the mutant shows altered time course data compared to the wild-type enzyme | Alopochen aegyptiaca |
N37G | site-directed mutagenesis at the substrate subsite F, the mutant shows a slightly altered product pattern compared to the wild-type enzyme | Alopochen aegyptiaca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aix sponsa | Q7LZQ2 | - |
- |
Alopochen aegyptiaca | P84496 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
egg white | - |
Alopochen aegyptiaca | - |
egg white | - |
Aix sponsa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitopentaose + H2O | - |
Alopochen aegyptiaca | N-acetyl-D-glucosamine + chitobiose + chitotetraose + chitotriose | amount in descending order, binding kinetics with chitotriose, overview | ? | |
chitopentaose + H2O | - |
Aix sponsa | N-acetyl-D-glucosamine + chitobiose + chitotriose + chitotetraose | amount in descending order, binding kinetics with chitotriose, overview | ? | |
lyophilized cell wall of Micrococcus luteus + H2O | - |
Alopochen aegyptiaca | ? | - |
? | |
lyophilized cell wall of Micrococcus luteus + H2O | - |
Aix sponsa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EGL | - |
Alopochen aegyptiaca |
Egyptian goose egg-white lysozyme | - |
Alopochen aegyptiaca |
Egyptian goose lysozyme | - |
Alopochen aegyptiaca |
WGL | - |
Aix sponsa |
wood duck lysozyme | - |
Aix sponsa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Alopochen aegyptiaca |
50 | - |
assay at | Aix sponsa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Alopochen aegyptiaca |
5 | - |
assay at | Aix sponsa |
General Information | Comment | Organism |
---|---|---|
additional information | rates of cleavage of glycosidic linkages, transglycosylation, and hydration, enzyme reaction modelling, overview | Alopochen aegyptiaca |
additional information | rates of cleavage of glycosidic linkages, transglycosylation, and hydration, enzyme reaction modelling, overview | Aix sponsa |