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Literature summary for 3.2.1.17 extracted from

  • Kawamura, S.; Toshima, G.; Chijiiwa, Y.; Torikata, T.; Araki, T.
    Amino acid sequence of Egyptian goose egg-white lysozyme and effects of amino acid substitution on the enzymatic activity (2012), Biosci. Biotechnol. Biochem., 76, 691-698.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, expression of enzyme mutants in Pichia pastoris, subcloning in Escherichia coli strain JM109 Alopochen aegyptiaca
sequence comparisons, phylogenetic tree Aix sponsa

Protein Variants

Protein Variants Comment Organism
F34Y site-directed mutagenesis at the substrate subsite E, the mutant shows a slightly altered product pattern compared to the wild-type enzyme Alopochen aegyptiaca
F34Y/N37G site-directed mutagenesis at the substrate subsites E/F, the mutant shows a slightly altered product pattern compared to the wild-type enzyme Alopochen aegyptiaca
F34Y/N37G/G71R site-directed mutagenesis, the mutant shows altered time course data compared to the wild-type enzyme Alopochen aegyptiaca
G71R site-directed mutagenesis, the mutant shows altered time course data compared to the wild-type enzyme Alopochen aegyptiaca
N37G site-directed mutagenesis at the substrate subsite F, the mutant shows a slightly altered product pattern compared to the wild-type enzyme Alopochen aegyptiaca

Organism

Organism UniProt Comment Textmining
Aix sponsa Q7LZQ2
-
-
Alopochen aegyptiaca P84496
-
-

Source Tissue

Source Tissue Comment Organism Textmining
egg white
-
Alopochen aegyptiaca
-
egg white
-
Aix sponsa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitopentaose + H2O
-
Alopochen aegyptiaca N-acetyl-D-glucosamine + chitobiose + chitotetraose + chitotriose amount in descending order, binding kinetics with chitotriose, overview ?
chitopentaose + H2O
-
Aix sponsa N-acetyl-D-glucosamine + chitobiose + chitotriose + chitotetraose amount in descending order, binding kinetics with chitotriose, overview ?
lyophilized cell wall of Micrococcus luteus + H2O
-
Alopochen aegyptiaca ?
-
?
lyophilized cell wall of Micrococcus luteus + H2O
-
Aix sponsa ?
-
?

Synonyms

Synonyms Comment Organism
EGL
-
Alopochen aegyptiaca
Egyptian goose egg-white lysozyme
-
Alopochen aegyptiaca
Egyptian goose lysozyme
-
Alopochen aegyptiaca
WGL
-
Aix sponsa
wood duck lysozyme
-
Aix sponsa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
assay at Alopochen aegyptiaca
50
-
assay at Aix sponsa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Alopochen aegyptiaca
5
-
assay at Aix sponsa

General Information

General Information Comment Organism
additional information rates of cleavage of glycosidic linkages, transglycosylation, and hydration, enzyme reaction modelling, overview Alopochen aegyptiaca
additional information rates of cleavage of glycosidic linkages, transglycosylation, and hydration, enzyme reaction modelling, overview Aix sponsa