Cloned (Comment) | Organism |
---|---|
acmB gene, AcmB devoid of its N-terminal domain, expression in Escherichia coli M15(pREP4), sequencing | Lactococcus lactis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | acmB deletion/insertion mutants with lower autolysis rate/extend | Lactococcus lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57100 | - |
x * 57100, sequence calculation | Lactococcus lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Lactococcus lactis | AcmB expression is modulated during cell growth, AcmB is not involved in cell separation but contributes to cellular autolysis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | Q8KKF9 | subsp. cremoris, strain MG1363 | - |
Purification (Comment) | Organism |
---|---|
recombinant AcmB | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | AcmB expression is modulated during cell growth, AcmB is not involved in cell separation but contributes to cellular autolysis | Lactococcus lactis | ? | - |
? | |
peptidoglycan + H2O | AcmB is an N-acetylglucosaminidase autolysin, three-domain modular structure, hydrolyzes peptidoglycans of several Gram-positive bacteria including Lactococcus lactis, AcmB hydrolyzes the peptidoglycan bonds in Bacillus subtilis HR vegetative cells between N-acetylglucosamine and N-acetylmuramic acid thus being an N-acetylglucosaminidase | Lactococcus lactis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57100, sequence calculation | Lactococcus lactis |
Synonyms | Comment | Organism |
---|---|---|
AcmB | - |
Lactococcus lactis |
N-acetylglucosaminidase autolysin | - |
Lactococcus lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Lactococcus lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
assay at | Lactococcus lactis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
active at acidic pH | Lactococcus lactis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Lactococcus lactis | sequence calculation | - |
4.9 |