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Literature summary for 3.2.1.169 extracted from

  • Mariappa, D.; Selvan, N.; Borodkin, V.; Alonso, J.; Ferenbach, A.T.; Shepherd, C.; Navratilova, I.H.; van Aalten, D.M.F.
    A mutant O-GlcNAcase as a probe to reveal global dynamics of protein O-GlcNAcylation during Drosophila embryonic development (2015), Biochem. J., 470, 255-262 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.169

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells Clostridium perfringens

Protein Variants

Protein Variants Comment Organism
D298N inactive Clostridium perfringens
D401A the mutation abrogates the interactions with the N-acetyl-D-glucosamine O4 and O6 hydroxyl groups23 Clostridium perfringens

Inhibitors

Inhibitors Comment Organism Structure
GlcNAcstatin B
-
 Clostridium perfringens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O Clostridium perfringens
-
 [protein]-L-serine + N-acetyl-D-glucosamine
-
 ?
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O Clostridium perfringens
-
 [protein]-L-threonine + N-acetyl-D-glucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium perfringens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography and Superdex S200 gel filtration Clostridium perfringens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4,6-triacetyl-O-GlcNAc-Fmoc-Ser-OH + H2O
-
 Clostridium perfringens ?
-
 ?
3,4,6-triacetyl-O-GlcNAc-Fmoc-Thr-OH + H2O
-
 Clostridium perfringens ?
-
 ?
[dHCF peptide]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O i.e. VPSgTMSAN Clostridium perfringens [dHCF peptide]-L-threonine + N-acetyl-D-glucosamine
-
 ?
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
-
 Clostridium perfringens [protein]-L-serine + N-acetyl-D-glucosamine
-
 ?
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O
-
 Clostridium perfringens [protein]-L-threonine + N-acetyl-D-glucosamine
-
 ?
[TAB1 protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
-
 Clostridium perfringens [TAB1 protein]-L-serine + N-acetyl-D-glucosamine
-
 ?

Synonyms

Synonyms Comment Organism
O-GlcNAcase
-
 Clostridium perfringens
OGA
-
 Clostridium perfringens