Cloned (Comment) | Organism |
---|---|
phylogenetic analysis, functional recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21 Star (DE3) cytosol as soluble protein | Actinoplanes missouriensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Actinoplanes missouriensis | |
Mg2+ | - |
Actinoplanes missouriensis | |
Zn2+ | - |
Actinoplanes missouriensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Actinoplanes missouriensis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 25% activation at 10 mM | Actinoplanes missouriensis | |
additional information | metal ions Mn2+, Ca2+, and Na+ at 1 mM do not affect the enzyme activity | Actinoplanes missouriensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hesperidin + H2O | Actinoplanes missouriensis | - |
hesperitin + rutinose | - |
? | |
hesperidin + H2O | Actinoplanes missouriensis NCIMB 12654 | - |
hesperitin + rutinose | - |
? | |
hesperidin + H2O | Actinoplanes missouriensis 431 | - |
hesperitin + rutinose | - |
? | |
hesperidin + H2O | Actinoplanes missouriensis NBRC 102363 | - |
hesperitin + rutinose | - |
? | |
hesperidin + H2O | Actinoplanes missouriensis DSM 43046 | - |
hesperitin + rutinose | - |
? | |
hesperidin + H2O | Actinoplanes missouriensis JCM 3121 | - |
hesperitin + rutinose | - |
? | |
hesperidin + H2O | Actinoplanes missouriensis CBS 188.64 | - |
hesperitin + rutinose | - |
? | |
hesperidin + H2O | Actinoplanes missouriensis ATCC 14538 | - |
hesperitin + rutinose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinoplanes missouriensis | I0GZ55 | isolated from sediment and soil samples collected from Antarctica (Base Marambio S56° 14' 31'' W56° 37' 48''), Patagonia Argentina (Rio Turbio S51° 31' 49'' W72° 21' 54'', El Calafate S50° 18' 36'' W72° 14' 42''), and Shala Lake (Ethiopia N7° 25' 30'' E38° 23' 42'') | - |
Actinoplanes missouriensis 431 | I0GZ55 | isolated from sediment and soil samples collected from Antarctica (Base Marambio S56° 14' 31'' W56° 37' 48''), Patagonia Argentina (Rio Turbio S51° 31' 49'' W72° 21' 54'', El Calafate S50° 18' 36'' W72° 14' 42''), and Shala Lake (Ethiopia N7° 25' 30'' E38° 23' 42'') | - |
Actinoplanes missouriensis ATCC 14538 | I0GZ55 | isolated from sediment and soil samples collected from Antarctica (Base Marambio S56° 14' 31'' W56° 37' 48''), Patagonia Argentina (Rio Turbio S51° 31' 49'' W72° 21' 54'', El Calafate S50° 18' 36'' W72° 14' 42''), and Shala Lake (Ethiopia N7° 25' 30'' E38° 23' 42'') | - |
Actinoplanes missouriensis CBS 188.64 | I0GZ55 | isolated from sediment and soil samples collected from Antarctica (Base Marambio S56° 14' 31'' W56° 37' 48''), Patagonia Argentina (Rio Turbio S51° 31' 49'' W72° 21' 54'', El Calafate S50° 18' 36'' W72° 14' 42''), and Shala Lake (Ethiopia N7° 25' 30'' E38° 23' 42'') | - |
Actinoplanes missouriensis DSM 43046 | I0GZ55 | isolated from sediment and soil samples collected from Antarctica (Base Marambio S56° 14' 31'' W56° 37' 48''), Patagonia Argentina (Rio Turbio S51° 31' 49'' W72° 21' 54'', El Calafate S50° 18' 36'' W72° 14' 42''), and Shala Lake (Ethiopia N7° 25' 30'' E38° 23' 42'') | - |
Actinoplanes missouriensis JCM 3121 | I0GZ55 | isolated from sediment and soil samples collected from Antarctica (Base Marambio S56° 14' 31'' W56° 37' 48''), Patagonia Argentina (Rio Turbio S51° 31' 49'' W72° 21' 54'', El Calafate S50° 18' 36'' W72° 14' 42''), and Shala Lake (Ethiopia N7° 25' 30'' E38° 23' 42'') | - |
Actinoplanes missouriensis NBRC 102363 | I0GZ55 | isolated from sediment and soil samples collected from Antarctica (Base Marambio S56° 14' 31'' W56° 37' 48''), Patagonia Argentina (Rio Turbio S51° 31' 49'' W72° 21' 54'', El Calafate S50° 18' 36'' W72° 14' 42''), and Shala Lake (Ethiopia N7° 25' 30'' E38° 23' 42'') | - |
Actinoplanes missouriensis NCIMB 12654 | I0GZ55 | isolated from sediment and soil samples collected from Antarctica (Base Marambio S56° 14' 31'' W56° 37' 48''), Patagonia Argentina (Rio Turbio S51° 31' 49'' W72° 21' 54'', El Calafate S50° 18' 36'' W72° 14' 42''), and Shala Lake (Ethiopia N7° 25' 30'' E38° 23' 42'') | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 Star (DE3) | Actinoplanes missouriensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:hesperidin-grown cell | - |
Actinoplanes missouriensis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.8 | - |
purified recombinant enzyme, substrate hesperidin, pH 7.0, 55°C | Actinoplanes missouriensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-beta-D-glucopyranoside + H2O | - |
Actinoplanes missouriensis | 4-methylumbelliferol + D-glucose | - |
? | |
4-methylumbelliferyl-beta-D-glucopyranoside + H2O | - |
Actinoplanes missouriensis NCIMB 12654 | 4-methylumbelliferol + D-glucose | - |
? | |
4-methylumbelliferyl-beta-D-glucopyranoside + H2O | - |
Actinoplanes missouriensis 431 | 4-methylumbelliferol + D-glucose | - |
? | |
4-methylumbelliferyl-beta-D-glucopyranoside + H2O | - |
Actinoplanes missouriensis NBRC 102363 | 4-methylumbelliferol + D-glucose | - |
? | |
4-methylumbelliferyl-beta-D-glucopyranoside + H2O | - |
Actinoplanes missouriensis DSM 43046 | 4-methylumbelliferol + D-glucose | - |
? | |
4-methylumbelliferyl-beta-D-glucopyranoside + H2O | - |
Actinoplanes missouriensis JCM 3121 | 4-methylumbelliferol + D-glucose | - |
? | |
4-methylumbelliferyl-beta-D-glucopyranoside + H2O | - |
Actinoplanes missouriensis CBS 188.64 | 4-methylumbelliferol + D-glucose | - |
? | |
4-methylumbelliferyl-beta-D-glucopyranoside + H2O | - |
Actinoplanes missouriensis ATCC 14538 | 4-methylumbelliferol + D-glucose | - |
? | |
4-methylumbelliferyl-beta-D-rutinoside + H2O | - |
Actinoplanes missouriensis | 4-methylumbelliferol + D-rutinose | - |
? | |
4-methylumbelliferyl-beta-D-rutinoside + H2O | - |
Actinoplanes missouriensis NCIMB 12654 | 4-methylumbelliferol + D-rutinose | - |
? | |
4-methylumbelliferyl-beta-D-rutinoside + H2O | - |
Actinoplanes missouriensis 431 | 4-methylumbelliferol + D-rutinose | - |
? | |
4-methylumbelliferyl-beta-D-rutinoside + H2O | - |
Actinoplanes missouriensis NBRC 102363 | 4-methylumbelliferol + D-rutinose | - |
? | |
4-methylumbelliferyl-beta-D-rutinoside + H2O | - |
Actinoplanes missouriensis DSM 43046 | 4-methylumbelliferol + D-rutinose | - |
? | |
4-methylumbelliferyl-beta-D-rutinoside + H2O | - |
Actinoplanes missouriensis JCM 3121 | 4-methylumbelliferol + D-rutinose | - |
? | |
4-methylumbelliferyl-beta-D-rutinoside + H2O | - |
Actinoplanes missouriensis CBS 188.64 | 4-methylumbelliferol + D-rutinose | - |
? | |
4-methylumbelliferyl-beta-D-rutinoside + H2O | - |
Actinoplanes missouriensis ATCC 14538 | 4-methylumbelliferol + D-rutinose | - |
? | |
4-nitrophenyl-beta-D-glucopyranoside + H2O | 1% activity compared to hesperidin | Actinoplanes missouriensis | 4-nitrophenol + D-glucose | - |
? | |
4-nitrophenyl-beta-D-rutinoside + H2O | 91% activity compared to hesperidin | Actinoplanes missouriensis | 4-nitrophenol + D-rutinose | - |
? | |
hesperidin + H2O | - |
Actinoplanes missouriensis | hesperitin + rutinose | - |
? | |
hesperidin + H2O | - |
Actinoplanes missouriensis NCIMB 12654 | hesperitin + rutinose | - |
? | |
hesperidin + H2O | - |
Actinoplanes missouriensis 431 | hesperitin + rutinose | - |
? | |
hesperidin + H2O | - |
Actinoplanes missouriensis NBRC 102363 | hesperitin + rutinose | - |
? | |
hesperidin + H2O | - |
Actinoplanes missouriensis DSM 43046 | hesperitin + rutinose | - |
? | |
hesperidin + H2O | - |
Actinoplanes missouriensis JCM 3121 | hesperitin + rutinose | - |
? | |
hesperidin + H2O | - |
Actinoplanes missouriensis CBS 188.64 | hesperitin + rutinose | - |
? | |
hesperidin + H2O | - |
Actinoplanes missouriensis ATCC 14538 | hesperitin + rutinose | - |
? | |
hesperidin methylchalcone + H2O | 91% activity compared to hesperidin | Actinoplanes missouriensis | hesperetin methylchalcone + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside | - |
? | |
hesperidin methylchalcone + H2O | 91% activity compared to hesperidin | Actinoplanes missouriensis NCIMB 12654 | hesperetin methylchalcone + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside | - |
? | |
hesperidin methylchalcone + H2O | 91% activity compared to hesperidin | Actinoplanes missouriensis 431 | hesperetin methylchalcone + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside | - |
? | |
hesperidin methylchalcone + H2O | 91% activity compared to hesperidin | Actinoplanes missouriensis NBRC 102363 | hesperetin methylchalcone + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside | - |
? | |
hesperidin methylchalcone + H2O | 91% activity compared to hesperidin | Actinoplanes missouriensis DSM 43046 | hesperetin methylchalcone + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside | - |
? | |
hesperidin methylchalcone + H2O | 91% activity compared to hesperidin | Actinoplanes missouriensis JCM 3121 | hesperetin methylchalcone + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside | - |
? | |
hesperidin methylchalcone + H2O | 91% activity compared to hesperidin | Actinoplanes missouriensis CBS 188.64 | hesperetin methylchalcone + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside | - |
? | |
hesperidin methylchalcone + H2O | 91% activity compared to hesperidin | Actinoplanes missouriensis ATCC 14538 | hesperetin methylchalcone + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside | - |
? | |
additional information | no activity with rutin, diosmin, isoquercitrin, naringin, or laminarin, and no activity with 4-nitrophenyl-alpha-L-rhamnopyranoside. The enzyme shows specificity for 7-O-rutinosylated flavonoids | Actinoplanes missouriensis | ? | - |
? | |
additional information | no activity with rutin, diosmin, isoquercitrin, naringin, or laminarin, and no activity with 4-nitrophenyl-alpha-L-rhamnopyranoside. The enzyme shows specificity for 7-O-rutinosylated flavonoids | Actinoplanes missouriensis NCIMB 12654 | ? | - |
? | |
additional information | no activity with rutin, diosmin, isoquercitrin, naringin, or laminarin, and no activity with 4-nitrophenyl-alpha-L-rhamnopyranoside. The enzyme shows specificity for 7-O-rutinosylated flavonoids | Actinoplanes missouriensis 431 | ? | - |
? | |
additional information | no activity with rutin, diosmin, isoquercitrin, naringin, or laminarin, and no activity with 4-nitrophenyl-alpha-L-rhamnopyranoside. The enzyme shows specificity for 7-O-rutinosylated flavonoids | Actinoplanes missouriensis NBRC 102363 | ? | - |
? | |
additional information | no activity with rutin, diosmin, isoquercitrin, naringin, or laminarin, and no activity with 4-nitrophenyl-alpha-L-rhamnopyranoside. The enzyme shows specificity for 7-O-rutinosylated flavonoids | Actinoplanes missouriensis DSM 43046 | ? | - |
? | |
additional information | no activity with rutin, diosmin, isoquercitrin, naringin, or laminarin, and no activity with 4-nitrophenyl-alpha-L-rhamnopyranoside. The enzyme shows specificity for 7-O-rutinosylated flavonoids | Actinoplanes missouriensis JCM 3121 | ? | - |
? | |
additional information | no activity with rutin, diosmin, isoquercitrin, naringin, or laminarin, and no activity with 4-nitrophenyl-alpha-L-rhamnopyranoside. The enzyme shows specificity for 7-O-rutinosylated flavonoids | Actinoplanes missouriensis CBS 188.64 | ? | - |
? | |
additional information | no activity with rutin, diosmin, isoquercitrin, naringin, or laminarin, and no activity with 4-nitrophenyl-alpha-L-rhamnopyranoside. The enzyme shows specificity for 7-O-rutinosylated flavonoids | Actinoplanes missouriensis ATCC 14538 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, recombinant His-tagged enzyme, SDS-PAGE, x * 60000, abouot, sequence calculation | Actinoplanes missouriensis |
More | peptide mapping | Actinoplanes missouriensis |
Synonyms | Comment | Organism |
---|---|---|
AMIS_8220 | - |
Actinoplanes missouriensis |
hesperidin deglycosylase | - |
Actinoplanes missouriensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Actinoplanes missouriensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | purified recombinant enzyme, stable for at least 1 h | Actinoplanes missouriensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Actinoplanes missouriensis |
Organism | Comment | Expression |
---|---|---|
Actinoplanes missouriensis | the enzyme is induced by growth on hesperidin | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycoside hydrolase family 55, GH55 | Actinoplanes missouriensis |
additional information | the hesperidin deglycosylation activity and diglycosidase production of the enzyme in Actinoplanes missouriensis strain 431 is 3 to 4 orders of magnitude higher than the corresponding monoglycosidase activities | Actinoplanes missouriensis |