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Literature summary for 3.2.1.168 extracted from

  • Neher, B.D.; Mazzaferro, L.S.; Kotik, M.; Oyhenart, J.; Halada, P.; Kren, V.; Breccia, J.D.
    Bacteria as source of diglycosidase activity Actinoplanes missouriensis produces 6-O-alpha-L-rhamnosyl-beta-D-glucosidase active on flavonoids (2016), Appl. Microbiol. Biotechnol., 100, 3061-3070 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic analysis, functional recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21 Star (DE3) cytosol as soluble protein Actinoplanes missouriensis

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Actinoplanes missouriensis
Mg2+
-
Actinoplanes missouriensis
Zn2+
-
Actinoplanes missouriensis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Actinoplanes missouriensis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 25% activation at 10 mM Actinoplanes missouriensis
additional information metal ions Mn2+, Ca2+, and Na+ at 1 mM do not affect the enzyme activity Actinoplanes missouriensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hesperidin + H2O Actinoplanes missouriensis
-
hesperitin + rutinose
-
?
hesperidin + H2O Actinoplanes missouriensis NCIMB 12654
-
hesperitin + rutinose
-
?
hesperidin + H2O Actinoplanes missouriensis 431
-
hesperitin + rutinose
-
?
hesperidin + H2O Actinoplanes missouriensis NBRC 102363
-
hesperitin + rutinose
-
?
hesperidin + H2O Actinoplanes missouriensis DSM 43046
-
hesperitin + rutinose
-
?
hesperidin + H2O Actinoplanes missouriensis JCM 3121
-
hesperitin + rutinose
-
?
hesperidin + H2O Actinoplanes missouriensis CBS 188.64
-
hesperitin + rutinose
-
?
hesperidin + H2O Actinoplanes missouriensis ATCC 14538
-
hesperitin + rutinose
-
?

Organism

Organism UniProt Comment Textmining
Actinoplanes missouriensis I0GZ55 isolated from sediment and soil samples collected from Antarctica (Base Marambio S56° 14' 31'' W56° 37' 48''), Patagonia Argentina (Rio Turbio S51° 31' 49'' W72° 21' 54'', El Calafate S50° 18' 36'' W72° 14' 42''), and Shala Lake (Ethiopia N7° 25' 30'' E38° 23' 42'')
-
Actinoplanes missouriensis 431 I0GZ55 isolated from sediment and soil samples collected from Antarctica (Base Marambio S56° 14' 31'' W56° 37' 48''), Patagonia Argentina (Rio Turbio S51° 31' 49'' W72° 21' 54'', El Calafate S50° 18' 36'' W72° 14' 42''), and Shala Lake (Ethiopia N7° 25' 30'' E38° 23' 42'')
-
Actinoplanes missouriensis ATCC 14538 I0GZ55 isolated from sediment and soil samples collected from Antarctica (Base Marambio S56° 14' 31'' W56° 37' 48''), Patagonia Argentina (Rio Turbio S51° 31' 49'' W72° 21' 54'', El Calafate S50° 18' 36'' W72° 14' 42''), and Shala Lake (Ethiopia N7° 25' 30'' E38° 23' 42'')
-
Actinoplanes missouriensis CBS 188.64 I0GZ55 isolated from sediment and soil samples collected from Antarctica (Base Marambio S56° 14' 31'' W56° 37' 48''), Patagonia Argentina (Rio Turbio S51° 31' 49'' W72° 21' 54'', El Calafate S50° 18' 36'' W72° 14' 42''), and Shala Lake (Ethiopia N7° 25' 30'' E38° 23' 42'')
-
Actinoplanes missouriensis DSM 43046 I0GZ55 isolated from sediment and soil samples collected from Antarctica (Base Marambio S56° 14' 31'' W56° 37' 48''), Patagonia Argentina (Rio Turbio S51° 31' 49'' W72° 21' 54'', El Calafate S50° 18' 36'' W72° 14' 42''), and Shala Lake (Ethiopia N7° 25' 30'' E38° 23' 42'')
-
Actinoplanes missouriensis JCM 3121 I0GZ55 isolated from sediment and soil samples collected from Antarctica (Base Marambio S56° 14' 31'' W56° 37' 48''), Patagonia Argentina (Rio Turbio S51° 31' 49'' W72° 21' 54'', El Calafate S50° 18' 36'' W72° 14' 42''), and Shala Lake (Ethiopia N7° 25' 30'' E38° 23' 42'')
-
Actinoplanes missouriensis NBRC 102363 I0GZ55 isolated from sediment and soil samples collected from Antarctica (Base Marambio S56° 14' 31'' W56° 37' 48''), Patagonia Argentina (Rio Turbio S51° 31' 49'' W72° 21' 54'', El Calafate S50° 18' 36'' W72° 14' 42''), and Shala Lake (Ethiopia N7° 25' 30'' E38° 23' 42'')
-
Actinoplanes missouriensis NCIMB 12654 I0GZ55 isolated from sediment and soil samples collected from Antarctica (Base Marambio S56° 14' 31'' W56° 37' 48''), Patagonia Argentina (Rio Turbio S51° 31' 49'' W72° 21' 54'', El Calafate S50° 18' 36'' W72° 14' 42''), and Shala Lake (Ethiopia N7° 25' 30'' E38° 23' 42'')
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 Star (DE3) Actinoplanes missouriensis

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:hesperidin-grown cell
-
Actinoplanes missouriensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8
-
purified recombinant enzyme, substrate hesperidin, pH 7.0, 55°C Actinoplanes missouriensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
Actinoplanes missouriensis 4-methylumbelliferol + D-glucose
-
?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
Actinoplanes missouriensis NCIMB 12654 4-methylumbelliferol + D-glucose
-
?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
Actinoplanes missouriensis 431 4-methylumbelliferol + D-glucose
-
?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
Actinoplanes missouriensis NBRC 102363 4-methylumbelliferol + D-glucose
-
?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
Actinoplanes missouriensis DSM 43046 4-methylumbelliferol + D-glucose
-
?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
Actinoplanes missouriensis JCM 3121 4-methylumbelliferol + D-glucose
-
?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
Actinoplanes missouriensis CBS 188.64 4-methylumbelliferol + D-glucose
-
?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
Actinoplanes missouriensis ATCC 14538 4-methylumbelliferol + D-glucose
-
?
4-methylumbelliferyl-beta-D-rutinoside + H2O
-
Actinoplanes missouriensis 4-methylumbelliferol + D-rutinose
-
?
4-methylumbelliferyl-beta-D-rutinoside + H2O
-
Actinoplanes missouriensis NCIMB 12654 4-methylumbelliferol + D-rutinose
-
?
4-methylumbelliferyl-beta-D-rutinoside + H2O
-
Actinoplanes missouriensis 431 4-methylumbelliferol + D-rutinose
-
?
4-methylumbelliferyl-beta-D-rutinoside + H2O
-
Actinoplanes missouriensis NBRC 102363 4-methylumbelliferol + D-rutinose
-
?
4-methylumbelliferyl-beta-D-rutinoside + H2O
-
Actinoplanes missouriensis DSM 43046 4-methylumbelliferol + D-rutinose
-
?
4-methylumbelliferyl-beta-D-rutinoside + H2O
-
Actinoplanes missouriensis JCM 3121 4-methylumbelliferol + D-rutinose
-
?
4-methylumbelliferyl-beta-D-rutinoside + H2O
-
Actinoplanes missouriensis CBS 188.64 4-methylumbelliferol + D-rutinose
-
?
4-methylumbelliferyl-beta-D-rutinoside + H2O
-
Actinoplanes missouriensis ATCC 14538 4-methylumbelliferol + D-rutinose
-
?
4-nitrophenyl-beta-D-glucopyranoside + H2O 1% activity compared to hesperidin Actinoplanes missouriensis 4-nitrophenol + D-glucose
-
?
4-nitrophenyl-beta-D-rutinoside + H2O 91% activity compared to hesperidin Actinoplanes missouriensis 4-nitrophenol + D-rutinose
-
?
hesperidin + H2O
-
Actinoplanes missouriensis hesperitin + rutinose
-
?
hesperidin + H2O
-
Actinoplanes missouriensis NCIMB 12654 hesperitin + rutinose
-
?
hesperidin + H2O
-
Actinoplanes missouriensis 431 hesperitin + rutinose
-
?
hesperidin + H2O
-
Actinoplanes missouriensis NBRC 102363 hesperitin + rutinose
-
?
hesperidin + H2O
-
Actinoplanes missouriensis DSM 43046 hesperitin + rutinose
-
?
hesperidin + H2O
-
Actinoplanes missouriensis JCM 3121 hesperitin + rutinose
-
?
hesperidin + H2O
-
Actinoplanes missouriensis CBS 188.64 hesperitin + rutinose
-
?
hesperidin + H2O
-
Actinoplanes missouriensis ATCC 14538 hesperitin + rutinose
-
?
hesperidin methylchalcone + H2O 91% activity compared to hesperidin Actinoplanes missouriensis hesperetin methylchalcone + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
?
hesperidin methylchalcone + H2O 91% activity compared to hesperidin Actinoplanes missouriensis NCIMB 12654 hesperetin methylchalcone + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
?
hesperidin methylchalcone + H2O 91% activity compared to hesperidin Actinoplanes missouriensis 431 hesperetin methylchalcone + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
?
hesperidin methylchalcone + H2O 91% activity compared to hesperidin Actinoplanes missouriensis NBRC 102363 hesperetin methylchalcone + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
?
hesperidin methylchalcone + H2O 91% activity compared to hesperidin Actinoplanes missouriensis DSM 43046 hesperetin methylchalcone + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
?
hesperidin methylchalcone + H2O 91% activity compared to hesperidin Actinoplanes missouriensis JCM 3121 hesperetin methylchalcone + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
?
hesperidin methylchalcone + H2O 91% activity compared to hesperidin Actinoplanes missouriensis CBS 188.64 hesperetin methylchalcone + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
?
hesperidin methylchalcone + H2O 91% activity compared to hesperidin Actinoplanes missouriensis ATCC 14538 hesperetin methylchalcone + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
?
additional information no activity with rutin, diosmin, isoquercitrin, naringin, or laminarin, and no activity with 4-nitrophenyl-alpha-L-rhamnopyranoside. The enzyme shows specificity for 7-O-rutinosylated flavonoids Actinoplanes missouriensis ?
-
?
additional information no activity with rutin, diosmin, isoquercitrin, naringin, or laminarin, and no activity with 4-nitrophenyl-alpha-L-rhamnopyranoside. The enzyme shows specificity for 7-O-rutinosylated flavonoids Actinoplanes missouriensis NCIMB 12654 ?
-
?
additional information no activity with rutin, diosmin, isoquercitrin, naringin, or laminarin, and no activity with 4-nitrophenyl-alpha-L-rhamnopyranoside. The enzyme shows specificity for 7-O-rutinosylated flavonoids Actinoplanes missouriensis 431 ?
-
?
additional information no activity with rutin, diosmin, isoquercitrin, naringin, or laminarin, and no activity with 4-nitrophenyl-alpha-L-rhamnopyranoside. The enzyme shows specificity for 7-O-rutinosylated flavonoids Actinoplanes missouriensis NBRC 102363 ?
-
?
additional information no activity with rutin, diosmin, isoquercitrin, naringin, or laminarin, and no activity with 4-nitrophenyl-alpha-L-rhamnopyranoside. The enzyme shows specificity for 7-O-rutinosylated flavonoids Actinoplanes missouriensis DSM 43046 ?
-
?
additional information no activity with rutin, diosmin, isoquercitrin, naringin, or laminarin, and no activity with 4-nitrophenyl-alpha-L-rhamnopyranoside. The enzyme shows specificity for 7-O-rutinosylated flavonoids Actinoplanes missouriensis JCM 3121 ?
-
?
additional information no activity with rutin, diosmin, isoquercitrin, naringin, or laminarin, and no activity with 4-nitrophenyl-alpha-L-rhamnopyranoside. The enzyme shows specificity for 7-O-rutinosylated flavonoids Actinoplanes missouriensis CBS 188.64 ?
-
?
additional information no activity with rutin, diosmin, isoquercitrin, naringin, or laminarin, and no activity with 4-nitrophenyl-alpha-L-rhamnopyranoside. The enzyme shows specificity for 7-O-rutinosylated flavonoids Actinoplanes missouriensis ATCC 14538 ?
-
?

Subunits

Subunits Comment Organism
? x * 60000, recombinant His-tagged enzyme, SDS-PAGE, x * 60000, abouot, sequence calculation Actinoplanes missouriensis
More peptide mapping Actinoplanes missouriensis

Synonyms

Synonyms Comment Organism
AMIS_8220
-
Actinoplanes missouriensis
hesperidin deglycosylase
-
Actinoplanes missouriensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
assay at Actinoplanes missouriensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 50 purified recombinant enzyme, stable for at least 1 h Actinoplanes missouriensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Actinoplanes missouriensis

Expression

Organism Comment Expression
Actinoplanes missouriensis the enzyme is induced by growth on hesperidin up

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycoside hydrolase family 55, GH55 Actinoplanes missouriensis
additional information the hesperidin deglycosylation activity and diglycosidase production of the enzyme in Actinoplanes missouriensis strain 431 is 3 to 4 orders of magnitude higher than the corresponding monoglycosidase activities Actinoplanes missouriensis