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Literature summary for 3.2.1.167 extracted from

  • Muderrisoglu, C.; Yesil-Celiktas, O.
    High-yield biocatalysis of baicalein 7-O-beta-D-glucuronide to baicalein using soluble Helix pomatia-derived beta-glucuronidase in a chemically defined acidic Medium (2019), Catal. Lett., 149, 1701-1709 .
No PubMed abstract available

Application

Application Comment Organism
synthesis the enzyme can be used for biotransformation of glycone baicalin to more pharmacologically active aglycone baicalein in extracts from Scutellaria baicalensis Georgi plant roots. Development of a chemically defined medium-based baicalein bioproduction process with a comparable yield compared to complex medium-based ones, overview Helix pomatia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
baicalin + H2O Helix pomatia
-
baicalein + D-glucuronate
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
dimethyl formamide enzyme HP-GUS retains its catalytic activity in presence of 5% DMF, which is required in the biotransformation assay due to a low solubility and insolubility problem of baicalin and baicalein at reaction pH 4.5, respectively Helix pomatia

Organism

Organism UniProt Comment Textmining
Helix pomatia
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
baicalin + H2O
-
Helix pomatia baicalein + D-glucuronate
-
?
baicalin + H2O baicalein 7-O-beta-D-glucuronide Helix pomatia baicalein + D-glucuronate
-
?
additional information development of a chemically defined medium-based baicalein bioproduction process with a comparable yield compared to complex medium-based ones, detailed overview Helix pomatia ?
-
?

Synonyms

Synonyms Comment Organism
beta-glucuronidase
-
Helix pomatia
GUS
-
Helix pomatia
HP-GUS
-
Helix pomatia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Helix pomatia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Helix pomatia