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Literature summary for 3.2.1.167 extracted from
- beta-Glucuronidase from Lactobacillus brevis useful for baicalin hydrolysis belongs to glycoside hydrolase family 30 (2014), Appl. Microbiol. Biotechnol., 98, 4021-4032.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3) | Levilactobacillus brevis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.154 | - |
4-nitrophenyl beta-D-glucuronide | native enzyme, pH 5.0, 37°C | Levilactobacillus brevis |  |
| 0.156 | - |
4-nitrophenyl beta-D-glucuronide | recombinant His-tagged enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
| 1.5 | 2 | Baicalin | native enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
| 1.74 | - |
Baicalin | recombinant His-tagged enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
| 2.02 | - |
wogonoside | recombinant His-tagged enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, native enzyme, SDS-PAGE | Levilactobacillus brevis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| baicalin + H2O | Levilactobacillus brevis | the enzyme prefers baicalin rather than estrone 3-(beta-D-glucuronide), one of the human endogenous steroid hormones | baicalein + D-glucuronate | - |
? | |
| baicalin + H2O | Levilactobacillus brevis FERM BP-4693 | the enzyme prefers baicalin rather than estrone 3-(beta-D-glucuronide), one of the human endogenous steroid hormones | baicalein + D-glucuronate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Levilactobacillus brevis | - |
subsp. coagulans | - |
| Levilactobacillus brevis FERM BP-4693 | - |
subsp. coagulans | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) by nickel affinity chromatography and dialysis, native enzyme 1800fold from cell-free extract by repeated anion exchange chromatography and gel filtration, followed by dialysis | Levilactobacillus brevis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.57 | - |
purified native enzyme, pH 5.0, 37°C, substrate baicalin | Levilactobacillus brevis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucuronide + H2O | - |
Levilactobacillus brevis | 4-nitrophenol + D-glucuronate | - |
? | |
| 4-nitrophenyl beta-D-glucuronide + H2O | - |
Levilactobacillus brevis FERM BP-4693 | 4-nitrophenol + D-glucuronate | - |
? | |
| baicalin + H2O | the enzyme prefers baicalin rather than estrone 3-(beta-D-glucuronide), one of the human endogenous steroid hormones | Levilactobacillus brevis | baicalein + D-glucuronate | - |
? | |
| baicalin + H2O | the enzyme is strictly glycon-specific but not aglycon-specific | Levilactobacillus brevis | baicalein + D-glucuronate | - |
? | |
| baicalin + H2O | the enzyme prefers baicalin rather than estrone 3-(beta-D-glucuronide), one of the human endogenous steroid hormones | Levilactobacillus brevis FERM BP-4693 | baicalein + D-glucuronate | - |
? | |
| baicalin + H2O | the enzyme is strictly glycon-specific but not aglycon-specific | Levilactobacillus brevis FERM BP-4693 | baicalein + D-glucuronate | - |
? | |
| estrone 3-(beta-D-glucuronide) + H2O | - |
Levilactobacillus brevis | estrone + D-glucuronate | - |
? | |
| estrone 3-(beta-D-glucuronide) + H2O | - |
Levilactobacillus brevis FERM BP-4693 | estrone + D-glucuronate | - |
? | |
| additional information | substrate specificity, the enzyme is inactive with other 4-nitrophenyl glycosides, overview | Levilactobacillus brevis | ? | - |
? | |
| additional information | substrate specificity, the enzyme is inactive with other 4-nitrophenyl glycosides, overview | Levilactobacillus brevis FERM BP-4693 | ? | - |
? | |
| wogonoside + H2O | - |
Levilactobacillus brevis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, native enzyme, SDS-PAGE | Levilactobacillus brevis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LcGUS30 | - |
Levilactobacillus brevis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Levilactobacillus brevis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | activity range, profile overview | Levilactobacillus brevis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | purified enzyme, stable at | Levilactobacillus brevis |
| 70 | - |
purified enzyme, inactivation | Levilactobacillus brevis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.37 | - |
wogonoside | recombinant His-tagged enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
| 18.5 | - |
Baicalin | recombinant His-tagged enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
| 21.3 | - |
Baicalin | native enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
| 31.8 | - |
4-nitrophenyl beta-D-glucuronide | recombinant His-tagged enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
| 53.7 | - |
4-nitrophenyl beta-D-glucuronide | native enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Levilactobacillus brevis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 7 | activity range, profile overview | Levilactobacillus brevis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
purified enzyme, inactivation | Levilactobacillus brevis |
| 3.5 | 9 | purified enzyme, stable at | Levilactobacillus brevis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycoside hydrolase family 30, GH30, subfamily 3 | Levilactobacillus brevis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.58 | - |
estrone 3-(beta-D-glucuronide) | recombinant His-tagged enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
| 3.15 | - |
wogonoside | recombinant His-tagged enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
| 10.6 | - |
Baicalin | recombinant His-tagged enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
| 14 | - |
Baicalin | native enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
| 204 | - |
4-nitrophenyl beta-D-glucuronide | recombinant His-tagged enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
| 348 | - |
4-nitrophenyl beta-D-glucuronide | native enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
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