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Literature summary for 3.2.1.167 extracted from
- Cloning and expression of beta-glucuronidase from Lactobacillus brevis in E. coli and application in the bioconversion of baicalin and wogonoside (2009), J. Microbiol. Biotechnol., 19, 1650-1655.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain GMS407 (beta-glucuronidase-deficient) | Levilactobacillus brevis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 71000 | - |
recombinant enzyme, SDS-PAGE | Levilactobacillus brevis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Levilactobacillus brevis | D2DMF3 | strain RO1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Levilactobacillus brevis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.284 | - |
at 37°C and pH 5.0, using 4-nitrophenyl-beta-D-glucuronide as substrate | Levilactobacillus brevis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucuronide + H2O | - |
Levilactobacillus brevis | 4-nitrophenol + beta-D-glucuronic acid | - |
? |  |
| baicalin + H2O | 0.15 mM of baicalin is completely transformed into baicalein within 3 h at 37°C, pH 5.0 | Levilactobacillus brevis | baicalein + D-glucuronate | - |
? | |
| wogonoside + H2O | 0.125 mM of wogonoside is completely transformed into wogonin within 3 h at 37°C, pH 5.0 | Levilactobacillus brevis | wogonin + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-glucuronidase | - |
Levilactobacillus brevis |
| GUS | - |
Levilactobacillus brevis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Levilactobacillus brevis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | the purified enzyme exhibits a half-life of 1 h at 60°C and more than 2 h at 50°C | Levilactobacillus brevis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Levilactobacillus brevis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | at 37°C, the GUS remains stable for 80 min at pH values ranging from 5.0 to 8.0. Activity decreases quickly above pH 7.0 | Levilactobacillus brevis |
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