KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0098 | - |
5,6,7-trihydroxyflavone 7-O-beta-D-glucoronate | pH 6.5, 28°C | Scutellaria baicalensis | |
0.0307 | - |
5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate | pH 6.5, 28°C | Scutellaria baicalensis | |
0.0391 | - |
5,7-dihydroxy-6-methoxyflavone-7-O-beta-D-glucuronate | pH 6.5, 28°C | Scutellaria baicalensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
4 * 55000, SDS-PAGE | Scutellaria baicalensis |
230000 | - |
gel filtration | Scutellaria baicalensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Scutellaria baicalensis | - |
Georgi | - |
Scutellaria baicalensis Georgi | - |
Georgi | - |
Purification (Comment) | Organism |
---|---|
- |
Scutellaria baicalensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
callus culture | - |
Scutellaria baicalensis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
173.4 | - |
- |
Scutellaria baicalensis |
Storage Stability | Organism |
---|---|
-20°C, 10 mM NaH2PO4/Na2HPO4 buffer, 1 mM mercaptoethanol, pH 7.0, 50% glycerol, 2 months, 10-20% loss of activity | Scutellaria baicalensis |
4°C, 10 mM NaH2PO4/Na2HPO4 buffer, 1 mM mercaptoethanol, pH 7.0, 50% glycerol, 2 weeks, complete loss of activity | Scutellaria baicalensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate + H2O | i.e. baicalin | Scutellaria baicalensis | 5,6,7-trihydroxyflavone + D-glucuronate | i.e. baicalein | ? | |
5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate + H2O | i.e. baicalin | Scutellaria baicalensis Georgi | 5,6,7-trihydroxyflavone + D-glucuronate | i.e. baicalein | ? | |
5,7-dihydroxy-6-methoxyflavone-7-O-beta-D-glucuronate + H2O | i.e. oroxylin 7-O-beta-D-glucuronide, i.e. 5-hydroxy-6-methoxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid | Scutellaria baicalensis | 5,7-dihydroxy-6-methoxyflavone + D-glucuronate | - |
? | |
5,7-dihydroxy-6-methoxyflavone-7-O-beta-D-glucuronate + H2O | i.e. oroxylin 7-O-beta-D-glucuronide, i.e. 5-hydroxy-6-methoxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid | Scutellaria baicalensis Georgi | 5,7-dihydroxy-6-methoxyflavone + D-glucuronate | - |
? | |
5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate + H2O | i.e. wogonin 7-O-beta-D-glucuronide, i.e. 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid | Scutellaria baicalensis | 5,7-dihydroxy-8-methoxyflavone + D-glucuronate | - |
? | |
5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate + H2O | i.e. wogonin 7-O-beta-D-glucuronide, i.e. 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid | Scutellaria baicalensis Georgi | 5,7-dihydroxy-8-methoxyflavone + D-glucuronate | - |
? | |
additional information | no activity with 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucoside, 4-nitrophenyl beta-D-glucuronide and luteolin 3'-O-beta-D-glucuronide | Scutellaria baicalensis | ? | - |
? | |
additional information | no activity with 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucoside, 4-nitrophenyl beta-D-glucuronide and luteolin 3'-O-beta-D-glucuronide | Scutellaria baicalensis Georgi | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 55000, SDS-PAGE | Scutellaria baicalensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Scutellaria baicalensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Scutellaria baicalensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
activity completely disappeares below pH 4.5 | Scutellaria baicalensis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Scutellaria baicalensis | isoelectric focusing | - |
5.5 |