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Literature summary for 3.2.1.167 extracted from

  • Morimoto, S.; Harioka, T.; Shoyama, Y.
    Purification and characterization of flavone-specific beta-glucuronidase from callus cultures of Scutellaria baicalensis Georgi (1995), Planta, 195, 535-540.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0098
-
5,6,7-trihydroxyflavone 7-O-beta-D-glucoronate pH 6.5, 28°C Scutellaria baicalensis
0.0307
-
5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate pH 6.5, 28°C Scutellaria baicalensis
0.0391
-
5,7-dihydroxy-6-methoxyflavone-7-O-beta-D-glucuronate pH 6.5, 28°C Scutellaria baicalensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
4 * 55000, SDS-PAGE Scutellaria baicalensis
230000
-
gel filtration Scutellaria baicalensis

Organism

Organism UniProt Comment Textmining
Scutellaria baicalensis
-
Georgi
-
Scutellaria baicalensis Georgi
-
Georgi
-

Purification (Commentary)

Purification (Comment) Organism
-
Scutellaria baicalensis

Source Tissue

Source Tissue Comment Organism Textmining
callus culture
-
Scutellaria baicalensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
173.4
-
-
Scutellaria baicalensis

Storage Stability

Storage Stability Organism
-20°C, 10 mM NaH2PO4/Na2HPO4 buffer, 1 mM mercaptoethanol, pH 7.0, 50% glycerol, 2 months, 10-20% loss of activity Scutellaria baicalensis
4°C, 10 mM NaH2PO4/Na2HPO4 buffer, 1 mM mercaptoethanol, pH 7.0, 50% glycerol, 2 weeks, complete loss of activity Scutellaria baicalensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate + H2O i.e. baicalin Scutellaria baicalensis 5,6,7-trihydroxyflavone + D-glucuronate i.e. baicalein ?
5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate + H2O i.e. baicalin Scutellaria baicalensis Georgi 5,6,7-trihydroxyflavone + D-glucuronate i.e. baicalein ?
5,7-dihydroxy-6-methoxyflavone-7-O-beta-D-glucuronate + H2O i.e. oroxylin 7-O-beta-D-glucuronide, i.e. 5-hydroxy-6-methoxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid Scutellaria baicalensis 5,7-dihydroxy-6-methoxyflavone + D-glucuronate
-
?
5,7-dihydroxy-6-methoxyflavone-7-O-beta-D-glucuronate + H2O i.e. oroxylin 7-O-beta-D-glucuronide, i.e. 5-hydroxy-6-methoxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid Scutellaria baicalensis Georgi 5,7-dihydroxy-6-methoxyflavone + D-glucuronate
-
?
5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate + H2O i.e. wogonin 7-O-beta-D-glucuronide, i.e. 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid Scutellaria baicalensis 5,7-dihydroxy-8-methoxyflavone + D-glucuronate
-
?
5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate + H2O i.e. wogonin 7-O-beta-D-glucuronide, i.e. 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid Scutellaria baicalensis Georgi 5,7-dihydroxy-8-methoxyflavone + D-glucuronate
-
?
additional information no activity with 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucoside, 4-nitrophenyl beta-D-glucuronide and luteolin 3'-O-beta-D-glucuronide Scutellaria baicalensis ?
-
?
additional information no activity with 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucoside, 4-nitrophenyl beta-D-glucuronide and luteolin 3'-O-beta-D-glucuronide Scutellaria baicalensis Georgi ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 55000, SDS-PAGE Scutellaria baicalensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Scutellaria baicalensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
Scutellaria baicalensis

pH Range

pH Minimum pH Maximum Comment Organism
4.5
-
activity completely disappeares below pH 4.5 Scutellaria baicalensis

pI Value

Organism Comment pI Value Maximum pI Value
Scutellaria baicalensis isoelectric focusing
-
5.5