Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. Escherichia coli having pET28a/sGUS shows much higher beta-glucuronidase activity using baicalein 7-O-beta-D-glucuronide as a substrate than Escherichia coli carrying pET28a. The minor enzyme activity observed in the latter is considered to be due to endogenous beta-glucuronidase | Scutellaria baicalensis |
Protein Variants | Comment | Organism |
---|---|---|
E194A | beta-glucuronidase activity of the mutant enzyme with baicalein 7-O-beta-D-glucuronide is 86% of wild-type activity | Scutellaria baicalensis |
E212A | beta-glucuronidase activity of the mutant enzyme with baicalein 7-O-beta-D-glucuronide is 1.2% of wild-type activity | Scutellaria baicalensis |
E225A | beta-glucuronidase activity of the mutant enzyme with baicalein 7-O-beta-D-glucuronide is 94% of wild-type activity | Scutellaria baicalensis |
E272A | beta-glucuronidase activity of the mutant enzyme with baicalein 7-O-beta-D-glucuronide is 70% of wild-type activity | Scutellaria baicalensis |
E329A | beta-glucuronidase activity of the mutant enzyme with baicalein 7-O-beta-D-glucuronide is 0.3% of wild-type activity | Scutellaria baicalensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0097 | - |
5,6,7-trihydroxyflavone 7-O-beta-D-glucoronate | pH 6.5, 28°C, mutant enzyme E329A | Scutellaria baicalensis | |
0.0103 | - |
5,6,7-trihydroxyflavone 7-O-beta-D-glucoronate | pH 6.5, 28°C, mutant enzyme E212A | Scutellaria baicalensis | |
0.0121 | - |
5,6,7-trihydroxyflavone 7-O-beta-D-glucoronate | pH 6.5, 28°C, wild-type enzyme | Scutellaria baicalensis | |
0.027 | - |
5,6,7-trihydroxyflavone 7-O-beta-D-glucoronate | pH 6.5, 28°C, mutant enzyme Y281A | Scutellaria baicalensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56027 | - |
x * 56027, calculated from sequence | Scutellaria baicalensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Scutellaria baicalensis | Q9LRC8 | protein sequence homology shows no significant similarity to the family 2 beta-glucuronidases | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Scutellaria baicalensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate + H2O | i.e. baicalin. The enzyme shows more than 20fold higher activity for baicalein 7-O-beta-D-glucuronide as compared with luteolin 3'-O-beta-D-glucuronide | Scutellaria baicalensis | 5,6,7-trihydroxyflavone + D-glucuronate | i.e. baicalein | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56027, calculated from sequence | Scutellaria baicalensis |
Synonyms | Comment | Organism |
---|---|---|
sGUS | - |
Scutellaria baicalensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Scutellaria baicalensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
5,6,7-trihydroxyflavone 7-O-beta-D-glucoronate | pH 6.5, 28°C, mutant enzyme E329A | Scutellaria baicalensis | |
0.135 | - |
5,6,7-trihydroxyflavone 7-O-beta-D-glucoronate | pH 6.5, 28°C, mutant enzyme E212A | Scutellaria baicalensis | |
0.77 | - |
5,6,7-trihydroxyflavone 7-O-beta-D-glucoronate | pH 6.5, 28°C, mutant enzyme Y281A | Scutellaria baicalensis | |
639 | - |
5,6,7-trihydroxyflavone 7-O-beta-D-glucoronate | pH 6.5, 28°C, wild-type enzyme | Scutellaria baicalensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Scutellaria baicalensis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Scutellaria baicalensis | calculated from sequence | - |
5.52 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
5,6,7-trihydroxyflavone 7-O-beta-D-glucoronate | pH 6.5, 28°C, mutant enzyme E329A | Scutellaria baicalensis | |
13.1 | - |
5,6,7-trihydroxyflavone 7-O-beta-D-glucoronate | pH 6.5, 28°C, mutant enzyme E212A | Scutellaria baicalensis | |
28.5 | - |
5,6,7-trihydroxyflavone 7-O-beta-D-glucoronate | pH 6.5, 28°C, mutant enzyme Y281A | Scutellaria baicalensis | |
52810 | - |
5,6,7-trihydroxyflavone 7-O-beta-D-glucoronate | pH 6.5, 28°C, wild-type enzyme | Scutellaria baicalensis |