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Literature summary for 3.2.1.165 extracted from

  • Wu, D.; Wang, L.; Li, Y.; Zhao, S.; Peng, N.; Liang, Y.
    Heterologous expression and characterization of a thermostable exo-beta-D-glucosaminidase from Aspergillus oryzae (2016), J. Microbiol. Biotechnol., 26, 347-355 .
    View publication on PubMed
Search for more literature for 3.2.1.165

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris GS115 cells and Escherichia coli Rosetta (DE3) pLysS cells Aspergillus oryzae

Protein Variants

Protein Variants Comment Organism
F769W the mutation significantly enhances the specific activity and hydrolysis velocity compared to the wild type enzyme Aspergillus oryzae

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 47.9% residual activity at 5 mg/ml, complete inhibition at 10 mg/ml Aspergillus oryzae
Ca2+ 76.8% residual activity at 10 mg/ml Aspergillus oryzae
Co2+ 4.8% residual activity at 10 mg/ml Aspergillus oryzae
Cu2+ 19.3% residual activity at 10 mg/ml Aspergillus oryzae
EDTA 65% residual activity at 1 mg/ml, complete inhibition at 5 mg/ml Aspergillus oryzae
Fe2+ 16.1% residual activity at 10 mg/ml Aspergillus oryzae
K+ 84.6% residual activity at 10 mg/ml Aspergillus oryzae
Mg2+ 55.8% residual activity at 10 mg/ml Aspergillus oryzae
Mn2+ 2.5% residual activity at 5 mg/ml, complete inhibition at 10 mg/ml Aspergillus oryzae
Ni2+ 82.9% residual activity at 10 mg/ml Aspergillus oryzae
Zn2+ 55.8% residual activity at 10 mg/ml Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
18.4
-
 colloidal chitosan wild type enzyme, at pH 5.5 and 50°C Aspergillus oryzae
32.9
-
 colloidal chitosan mutant enzyme F769W, at pH 5.5 and 50°C Aspergillus oryzae

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not influenced by Ba2+ Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae W8E1X9
-
-
Aspergillus oryzae FL402 W8E1X9
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
colloidal chitosan + H2O
-
 Aspergillus oryzae beta-D-glucosamine + ?
-
 ?
colloidal chitosan + H2O
-
 Aspergillus oryzae FL402 beta-D-glucosamine + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 100000, SDS-PAGE Aspergillus oryzae
? x * 99200, calculated from amino acid sequence Aspergillus oryzae

Synonyms

Synonyms Comment Organism
CsxA
-
 Aspergillus oryzae
exo-beta-D-glucosaminidase
-
 Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Aspergillus oryzae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60 the recombinant enzyme expressed in Escherichia coli shows more than 45% activity between 40 and 60°C. The recombinant enzyme expressed in Pichia pastoris shows more than 70% activity between 40 and 60°C Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 the recombinant enzyme from Pichia pastoris remains about 80% activity after 25 h at 50°C, while the recombinant enzyme from Escherichia coli is completely inactive (0.48% residual activity) after 3 h at 50°C Aspergillus oryzae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23.8
-
 colloidal chitosan wild type enzyme, at pH 5.5 and 50°C Aspergillus oryzae
102.6
-
 colloidal chitosan mutant enzyme F769W, at pH 5.5 and 50°C Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Aspergillus oryzae

pH Range

pH Minimum pH Maximum Comment Organism
4 8 the recombinant enzyme expressed in Escherichia coli shows about 40% activity at pH 4.0, about 80% activity at pH 5.0, 100% activity at pH 5.5, about 90% activity at pH 6.0, and about 20% activity at pH 8.0. The recombinant enzyme expressed in Pichia pastoris shows about 70% activity at pH 4.0, about 90% activity at pH 5.0, 100% activity at pH 5.5, about 95% activity at pH 6.0, and about 20% activity at pH 8.0 Aspergillus oryzae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.29
-
 colloidal chitosan wild type enzyme, at pH 5.5 and 50°C Aspergillus oryzae
3.11
-
 colloidal chitosan mutant enzyme F769W, at pH 5.5 and 50°C Aspergillus oryzae