BRENDA - Enzyme Database
show all sequences of 3.2.1.162

Efficient enzymatic degradation process for hydrolysis activity of the carrageenan from red algae in marine biomass

Kang, D.H.; Hyeon, J.E.; You, S.K.; Kim, S.W.; Han, S.O.; J. Biotechnol. 192 Pt A, 108-113 (2014)

Data extracted from this reference:

Application
Application
Commentary
Organism
agriculture
the assemblies of advancement of active enzyme complexes, i.e. of kappa-carrageenase CgkA and delta-carrageenase CglA, will facilitate the commercial production of useful products from red algae biomass whichrepresents inexpensive and sustainable feed-stocks
Pseudoalteromonas carrageenovora
Cloned(Commentary)
Commentary
Organism
subcloning in Escherichia coli strain DH5alpha, recombinant expression of a chimeric protein cCgkA and cCglA containing the catalytic domain of kappa-carrageenase CgkA and delta-carrageenase CglA fused with a dockerin domain in Escherichia coli strain BL21(DE3)
Pseudoalteromonas carrageenovora
Engineering
Amino acid exchange
Commentary
Organism
additional information
generation of a chimeric protein cCgkA and cCglA containing the catalytic domain of kappa-carrageenase CgkA and delta-carrageenase CglA fused with a dockerin domain, miniCbpA, cCgkA and cCglA assemble into a complex, the dockerin-fused enzymes on the scaffoldin have synergistic activity in the degradationof carrageenan showing enhancement of activity by carrageenolytic complex 3.1fold higher compared with the corresponding enzymes alone
Pseudoalteromonas carrageenovora
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
109000
-
gel filtration
Pseudoalteromonas carrageenovora
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudoalteromonas carrageenovora
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant chimeric protein cCgkA and cCglA containing the catalytic domain of kappa-carrageenase CgkA and delta-carrageenase CglA fused with a dockerin domain from Escherichia coli strain BL21(DE3) by cellulose affinity chromatography using carbohydrate binding module in scaffoldin miniCbpA as a tag
Pseudoalteromonas carrageenovora
Subunits
Subunits
Commentary
Organism
?
x * 109000, His-tagged enzyme, SDS-PAGE
Pseudoalteromonas carrageenovora
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
assay at
Pseudoalteromonas carrageenovora
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Pseudoalteromonas carrageenovora
Application (protein specific)
Application
Commentary
Organism
agriculture
the assemblies of advancement of active enzyme complexes, i.e. of kappa-carrageenase CgkA and delta-carrageenase CglA, will facilitate the commercial production of useful products from red algae biomass whichrepresents inexpensive and sustainable feed-stocks
Pseudoalteromonas carrageenovora
Cloned(Commentary) (protein specific)
Commentary
Organism
subcloning in Escherichia coli strain DH5alpha, recombinant expression of a chimeric protein cCgkA and cCglA containing the catalytic domain of kappa-carrageenase CgkA and delta-carrageenase CglA fused with a dockerin domain in Escherichia coli strain BL21(DE3)
Pseudoalteromonas carrageenovora
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
generation of a chimeric protein cCgkA and cCglA containing the catalytic domain of kappa-carrageenase CgkA and delta-carrageenase CglA fused with a dockerin domain, miniCbpA, cCgkA and cCglA assemble into a complex, the dockerin-fused enzymes on the scaffoldin have synergistic activity in the degradationof carrageenan showing enhancement of activity by carrageenolytic complex 3.1fold higher compared with the corresponding enzymes alone
Pseudoalteromonas carrageenovora
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
109000
-
gel filtration
Pseudoalteromonas carrageenovora
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant chimeric protein cCgkA and cCglA containing the catalytic domain of kappa-carrageenase CgkA and delta-carrageenase CglA fused with a dockerin domain from Escherichia coli strain BL21(DE3) by cellulose affinity chromatography using carbohydrate binding module in scaffoldin miniCbpA as a tag
Pseudoalteromonas carrageenovora
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 109000, His-tagged enzyme, SDS-PAGE
Pseudoalteromonas carrageenovora
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
assay at
Pseudoalteromonas carrageenovora
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Pseudoalteromonas carrageenovora
Other publictions for EC 3.2.1.162
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731525
Li
Purification and characterizat ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) Lc50-1
Biotechnol. Lett.
36
1669-1674
2014
-
-
-
-
-
-
7
-
1
4
1
2
-
5
-
-
1
-
-
-
1
-
2
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
1
4
1
2
-
-
-
1
-
-
1
-
2
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
732212
Kang
Efficient enzymatic degradatio ...
Pseudoalteromonas carrageenovora
J. Biotechnol.
192 Pt A
108-113
2014
-
1
1
-
1
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
667539
Guibet
Degradation of lambda-carragee ...
Pseudoalteromonas carrageenovora, Pseudoalteromonas carrageenovora ATCC 43555
Biochem. J.
404
105-114
2007
-
-
1
-
-
-
-
-
1
-
3
2
-
4
-
-
1
1
-
1
-
-
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
4
2
-
-
-
2
-
1
-
-
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668168
Guibet
Complete assignment of 1H and ...
Pseudoalteromonas carrageenovora
Carbohydr. Res.
341
1859-1869
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
669161
Ohta
A novel enzyme, lambda-carrage ...
Pseudoalteromonas sp., Pseudoalteromonas sp. CL19
J. Biochem.
140
475-481
2006
-
-
1
-
-
1
-
-
-
5
1
-
-
4
-
-
1
-
-
1
-
-
2
1
1
1
2
-
1
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
5
1
-
-
-
-
1
-
1
-
-
2
1
1
1
2
-
1
1
-
-
-
-
-
-
-
-
668129
Johnston
Enzymic hydrolysis of the pota ...
Pseudomonas carrageenovora
Can. J. Microbiol.
19
779-788
1973
-
-
-
-
-
1
2
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-