Literature summary for 3.2.1.162 extracted from

Kang, D.H.; Hyeon, J.E.; You, S.K.; Kim, S.W.; Han, S.O.
Efficient enzymatic degradation process for hydrolysis activity of the carrageenan from red algae in marine biomass (2014), J. Biotechnol., 192 Pt A, 108-113.

Search for more literature for 3.2.1.162

Application

Application	Comment	Organism
agriculture	the assemblies of advancement of active enzyme complexes, i.e. of kappa-carrageenase CgkA and delta-carrageenase CglA, will facilitate the commercial production of useful products from red algae biomass whichrepresents inexpensive and sustainable feed-stocks	Pseudoalteromonas carrageenovora

Cloned(Commentary)

Cloned (Comment)	Organism
subcloning in Escherichia coli strain DH5alpha, recombinant expression of a chimeric protein cCgkA and cCglA containing the catalytic domain of kappa-carrageenase CgkA and delta-carrageenase CglA fused with a dockerin domain in Escherichia coli strain BL21(DE3)	Pseudoalteromonas carrageenovora

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of a chimeric protein cCgkA and cCglA containing the catalytic domain of kappa-carrageenase CgkA and delta-carrageenase CglA fused with a dockerin domain, miniCbpA, cCgkA and cCglA assemble into a complex, the dockerin-fused enzymes on the scaffoldin have synergistic activity in the degradationof carrageenan showing enhancement of activity by carrageenolytic complex 3.1fold higher compared with the corresponding enzymes alone	Pseudoalteromonas carrageenovora

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
109000	-	gel filtration	Pseudoalteromonas carrageenovora

Organism

Organism	UniProt	Comment	Textmining
Pseudoalteromonas carrageenovora	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant chimeric protein cCgkA and cCglA containing the catalytic domain of kappa-carrageenase CgkA and delta-carrageenase CglA fused with a dockerin domain from Escherichia coli strain BL21(DE3) by cellulose affinity chromatography using carbohydrate binding module in scaffoldin miniCbpA as a tag	Pseudoalteromonas carrageenovora

Purification (Comment)

Organism

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant chimeric protein cCgkA and cCglA containing the catalytic domain of kappa-carrageenase CgkA and delta-carrageenase CglA fused with a dockerin domain from Escherichia coli strain BL21(DE3) by cellulose affinity chromatography using carbohydrate binding module in scaffoldin miniCbpA as a tag

Pseudoalteromonas carrageenovora

Subunits

Subunits	Comment	Organism
?	x * 109000, His-tagged enzyme, SDS-PAGE	Pseudoalteromonas carrageenovora

Synonyms

Synonyms	Comment	Organism
CglA	-	Pseudoalteromonas carrageenovora
delta-carrageenase	-	Pseudoalteromonas carrageenovora

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	assay at	Pseudoalteromonas carrageenovora

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Pseudoalteromonas carrageenovora