Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Bacillus sp. (in: Bacteria) | |
Cu2+ | - |
Bacillus sp. (in: Bacteria) | |
EDTA | - |
Bacillus sp. (in: Bacteria) | |
Fe2+ | - |
Bacillus sp. (in: Bacteria) | |
Mg2+ | - |
Bacillus sp. (in: Bacteria) | |
Mn2+ | - |
Bacillus sp. (in: Bacteria) | |
Sr2+ | - |
Bacillus sp. (in: Bacteria) |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Bacillus sp. (in: Bacteria) | |
Co2+ | activates | Bacillus sp. (in: Bacteria) | |
K+ | activates | Bacillus sp. (in: Bacteria) | |
Na+ | activates | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
delta-carrageenan + H2O | Bacillus sp. (in: Bacteria) | Cga-L50 is an endo-type delta-carrageenase that hydrolyzes beta-1,4-linkages of kappa-carrageenan, yielding neo-delta-carrabiose as the main product | neo-delta-carrabiose + ? | - |
? | |
delta-carrageenan + H2O | Bacillus sp. (in: Bacteria) Lc50-1 | Cga-L50 is an endo-type delta-carrageenase that hydrolyzes beta-1,4-linkages of kappa-carrageenan, yielding neo-delta-carrabiose as the main product | neo-delta-carrabiose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
a thermophilic strain isolated from a hot spring in Indonesia | - |
Bacillus sp. (in: Bacteria) Lc50-1 | - |
a thermophilic strain isolated from a hot spring in Indonesia | - |
Purification (Comment) | Organism |
---|---|
native enzyme 37.8fold from culture supernatant, by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
105.9 | - |
purified native enzyme, pH 7.5, 75°C | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
delta-carrageenan + H2O | Cga-L50 is an endo-type delta-carrageenase that hydrolyzes beta-1,4-linkages of kappa-carrageenan, yielding neo-delta-carrabiose as the main product | Bacillus sp. (in: Bacteria) | neo-delta-carrabiose + ? | - |
? | |
delta-carrageenan + H2O | Cga-L50 is an endo-type delta-carrageenase that hydrolyzes beta-1,4-linkages of kappa-carrageenan, yielding neo-delta-carrabiose as the main product | Bacillus sp. (in: Bacteria) Lc50-1 | neo-delta-carrabiose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
Cga-L50 | - |
Bacillus sp. (in: Bacteria) |
delta-carrageenase | - |
Bacillus sp. (in: Bacteria) |
endo-type delta-carrageenase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
purified native enzyme, 10 min, over 50% activity remaining | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | purified native enzyme, stable at | Bacillus sp. (in: Bacteria) |