BRENDA - Enzyme Database
show all sequences of 3.2.1.162

A novel enzyme, lambda-carrageenase, isolated from a deep-sea bacterium

Ohta, Y.; Hatada, Y.; J. Biochem. 140, 475-481 (2006)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Pseudoalteromonas sp.
General Stability
General Stability
Organism
sorbitol, sucrose and trehalose improve stability
Pseudoalteromonas sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
CsCl
maximal activity in presence of 0.4 M NaCl. CsCl is able to compensate for KCl
Pseudoalteromonas sp.
KCl
maximal activity in presence of 0.4 M NaCl. KCl is able to compensate for KCl
Pseudoalteromonas sp.
LiCl
maximal activity in presence of 0.4 M NaCl. LiCl is able to compensate for KCl
Pseudoalteromonas sp.
NaCl
maximal activity in presence of 0.4 M NaCl
Pseudoalteromonas sp.
RbCl
maximal activity in presence of 0.4 M NaCl. RbCl is able to compensate for KCl
Pseudoalteromonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
100000
-
gel filtration
Pseudoalteromonas sp.
Organism
Organism
UniProt
Commentary
Textmining
Pseudoalteromonas sp.
Q05JY7
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Pseudoalteromonas sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
containing lambda-carrageenan as a carbon source
Pseudoalteromonas sp.
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
lambda-carrageenan + H2O
-
669161
Pseudoalteromonas sp.
alpha-D-Galp2,6S2-(1-3)-beta-D-Galp2S-(1-4)-alpha-D-Galp2,6S2-(1-3)-D-Galp2S + ?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 100000, SDS-PAGE
Pseudoalteromonas sp.
Synonyms
Synonyms
Commentary
Organism
endo-beta-1,4-carrageenose 2,6,2'-trisulfate-hydrolase
-
Pseudoalteromonas sp.
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
35
-
-
Pseudoalteromonas sp.
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
20
40
20C: about 40% of maximal activity, 40C: about 65% of maximal activity
Pseudoalteromonas sp.
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
25
-
120 min, stable
Pseudoalteromonas sp.
30
-
0.5 M sorbitol, 120 min, enzyme is stable up to
Pseudoalteromonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Pseudoalteromonas sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
8.5
pH 6.0: about 40% of maximal activity, pH 8.5: about 45% of maximal activity
Pseudoalteromonas sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pseudoalteromonas sp.
General Stability (protein specific)
General Stability
Organism
sorbitol, sucrose and trehalose improve stability
Pseudoalteromonas sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
CsCl
maximal activity in presence of 0.4 M NaCl. CsCl is able to compensate for KCl
Pseudoalteromonas sp.
KCl
maximal activity in presence of 0.4 M NaCl. KCl is able to compensate for KCl
Pseudoalteromonas sp.
LiCl
maximal activity in presence of 0.4 M NaCl. LiCl is able to compensate for KCl
Pseudoalteromonas sp.
NaCl
maximal activity in presence of 0.4 M NaCl
Pseudoalteromonas sp.
RbCl
maximal activity in presence of 0.4 M NaCl. RbCl is able to compensate for KCl
Pseudoalteromonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
100000
-
gel filtration
Pseudoalteromonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudoalteromonas sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
containing lambda-carrageenan as a carbon source
Pseudoalteromonas sp.
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
lambda-carrageenan + H2O
-
669161
Pseudoalteromonas sp.
alpha-D-Galp2,6S2-(1-3)-beta-D-Galp2S-(1-4)-alpha-D-Galp2,6S2-(1-3)-D-Galp2S + ?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 100000, SDS-PAGE
Pseudoalteromonas sp.
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
35
-
-
Pseudoalteromonas sp.
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
20
40
20C: about 40% of maximal activity, 40C: about 65% of maximal activity
Pseudoalteromonas sp.
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
25
-
120 min, stable
Pseudoalteromonas sp.
30
-
0.5 M sorbitol, 120 min, enzyme is stable up to
Pseudoalteromonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Pseudoalteromonas sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
8.5
pH 6.0: about 40% of maximal activity, pH 8.5: about 45% of maximal activity
Pseudoalteromonas sp.
Other publictions for EC 3.2.1.162
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
753336
Zhu
Insight into carrageenases ma ...
Pseudoalteromonas carrageenovora, Pseudoalteromonas sp. CL19
Crit. Rev. Biotechnol.
38
1261-1276
2018
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2
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2
2
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2
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4
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5
2
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2
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2
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2
2
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4
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2
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2
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2
2
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-
755018
Ghanbarzadeh
-
Carrageenans and carrageenase ...
Bacillus sp. Lc50-1
Phytochem. Rev.
17
535-571
2018
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2
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1
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1
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1
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2
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2
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1
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1
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2
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1
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1
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2
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2
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1
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1
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2
2
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731525
Li
Purification and characterizat ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) Lc50-1
Biotechnol. Lett.
36
1669-1674
2014
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7
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1
4
1
2
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5
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1
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1
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2
1
3
1
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1
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1
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1
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7
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1
4
1
2
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1
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1
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2
1
1
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1
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1
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1
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732212
Kang
Efficient enzymatic degradatio ...
Pseudoalteromonas carrageenovora
J. Biotechnol.
192 Pt A
108-113
2014
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1
1
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1
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1
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1
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1
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1
2
1
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1
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1
1
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1
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1
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1
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1
1
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1
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667539
Guibet
Degradation of lambda-carragee ...
Pseudoalteromonas carrageenovora, Pseudoalteromonas carrageenovora ATCC 43555
Biochem. J.
404
105-114
2007
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1
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1
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3
2
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4
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1
1
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1
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7
1
2
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2
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1
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4
2
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2
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1
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7
1
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668168
Guibet
Complete assignment of 1H and ...
Pseudoalteromonas carrageenovora
Carbohydr. Res.
341
1859-1869
2006
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3
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1
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1
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669161
Ohta
A novel enzyme, lambda-carrage ...
Pseudoalteromonas sp.
J. Biochem.
140
475-481
2006
-
-
1
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1
-
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-
5
1
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3
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1
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1
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1
1
1
1
1
2
-
1
1
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1
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1
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5
1
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1
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1
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1
1
1
1
2
-
1
1
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668129
Johnston
Enzymic hydrolysis of the pota ...
Pseudomonas carrageenovora
Can. J. Microbiol.
19
779-788
1973
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1
2
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1
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1
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1
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