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Literature summary for 3.2.1.159 extracted from

  • Sugano, Y.; Kodama, H.; Terada, I.; Yamazaki, Y.; Noma, M.
    Purification and characterization of a novel enzyme, alpha-neoagarooligosaccharide hydrolase (alpha-NAOS hydrolase), from a marine bacterium, Vibrio sp. strain JT0107 (1994), J. Bacteriol., 176, 6812-6818.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.159

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.37
-
 neoagarobiose pH 7.7, 30°C Vibrio sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 2 * 42000, SDS-PAGE Vibrio sp.
84000
-
 gel filtration Vibrio sp.

Organism

Organism UniProt Comment Textmining
Vibrio sp.
-
-
-
Vibrio sp. JT0107
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vibrio sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
92
-
 pH 7.7, 30°C Vibrio sp.

Storage Stability

Storage Stability Organism
-20°C, phosphate buffer pH 7.8, 30% glycerol, 2 months, 60% residual activity Vibrio sp.
4°C, phosphate buffer pH 7.8, 3 weeks, 60% residual activity, 2 months, 30% residual activity Vibrio sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-1,3-neoagarooligosaccharide + H2O
-
 Vibrio sp. agaropentaose + agarotriose + agarobiose + D-galactose
-
 ?
alpha-1,3-neoagarooligosaccharide + H2O
-
 Vibrio sp. JT0107 agaropentaose + agarotriose + agarobiose + D-galactose
-
 ?
neoagarobiose + H2O
-
 Vibrio sp. ?
-
 ?
neoagarobiose + H2O
-
 Vibrio sp. JT0107 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 42000, SDS-PAGE Vibrio sp.
More N-terminal amino acid sequence Vibrio sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Vibrio sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 120 min, stable Vibrio sp.
40
-
 rapid loss of activity Vibrio sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
-
 Vibrio sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.2 8.9 drastical reduction in activity below or above Vibrio sp.

pI Value

Organism Comment pI Value Maximum pI Value
Vibrio sp. isoelectric focusing
-
 4.6