Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli AD494(DE3) cells | Thalassomonas sp. LD5 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 3% residual activity at 2 mM | Thalassomonas sp. LD5 | |
Ba2+ | 90% residual activity at 2 mM | Thalassomonas sp. LD5 | |
Cu2+ | 5% residual activity at 2 mM | Thalassomonas sp. LD5 | |
EDTA | 1% residual activity at 2 mM | Thalassomonas sp. LD5 | |
Fe2+ | 18% residual activity at 2 mM | Thalassomonas sp. LD5 | |
Fe3+ | complete inhibition at 2 mM | Thalassomonas sp. LD5 | |
Mg2+ | 70% residual activity at 2 mM | Thalassomonas sp. LD5 | |
Mn2+ | complete inhibition at 2 mM | Thalassomonas sp. LD5 | |
NH4+ | 84% residual activity at 2 mM | Thalassomonas sp. LD5 | |
Ni2+ | 28% residual activity at 2 mM | Thalassomonas sp. LD5 | |
SDS | 39% residual activity at 2 mM | Thalassomonas sp. LD5 | |
Zn2+ | 10% residual activity at 2 mM | Thalassomonas sp. LD5 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the enzyme is strongly dependent on the presence of Ca2+ ions (170% activity at 10 mM) | Thalassomonas sp. LD5 | |
Li+ | 105% activity at 2 mM | Thalassomonas sp. LD5 | |
Na+ | 107% activity at 2 mM | Thalassomonas sp. LD5 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
360000 | - |
gel filtration | Thalassomonas sp. LD5 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thalassomonas sp. LD5 | A0A1S5VH75 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography and Superdex S200 gel filtration | Thalassomonas sp. LD5 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agar + H2O | - |
Thalassomonas sp. LD5 | agarotetraose | - |
? | |
agarotetraose + H2O | - |
Thalassomonas sp. LD5 | neoagarotriose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 180000, SDS-PAGE | Thalassomonas sp. LD5 |
homodimer | 2 * 154000, calculated from amino acid sequence | Thalassomonas sp. LD5 |
Synonyms | Comment | Organism |
---|---|---|
AgaD | - |
Thalassomonas sp. LD5 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Thalassomonas sp. LD5 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 45 | more than 50% of the enzyme activity is lost below 30°C and above 45°C | Thalassomonas sp. LD5 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 40 | the enzyme retains 100% or 60% activity after being incubated at 10-20°C or 30°C for 1 h, respectively. The enzyme loses nearly 95% activity after being incubated at 40°C for 1 h | Thalassomonas sp. LD5 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Thalassomonas sp. LD5 |