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Literature summary for 3.2.1.158 extracted from
- Agarase: review of major sources, categories, purification method, enzyme characteristics and applications (2010), Mar. drugs, 8, 200-218.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Bacillus subtilis | Thalassotalea agarivorans |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Alteromonas agarilytica | - |
- |
| intracellular | - |
Thalassotalea agarivorans | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
native enzyme | Thalassotalea agarivorans |
| 87000 | - |
recombinant enzyme | Thalassotalea agarivorans |
| 180000 | - |
2 * 180000, SDS-PAGE | Alteromonas agarilytica |
| 360000 | - |
gel filtration | Alteromonas agarilytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alteromonas agarilytica | Q9LAP7 | - |
- |
| Alteromonas agarilytica GJ1B | Q9LAP7 | - |
- |
| Thalassotalea agarivorans | A1IGV8 | - |
- |
| Thalassotalea agarivorans JAMB-A33 | A1IGV8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| hydroxyapatite chromatography and gel filtration | Thalassotalea agarivorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | - |
Alteromonas agarilytica | agarotetraose + ? | agarotetraose is the main product | ? |  |
| agarose + H2O | - |
Alteromonas agarilytica GJ1B | agarotetraose + ? | agarotetraose is the main product | ? | |
| agarose + H2O | - |
Thalassotalea agarivorans | agarotetraose + agarobiose + agarohexaose | agarotetraose is the main product | ? | |
| agarose + H2O | - |
Thalassotalea agarivorans JAMB-A33 | agarotetraose + agarobiose + agarohexaose | agarotetraose is the main product | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 180000, SDS-PAGE | Alteromonas agarilytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AgaA33 | - |
Thalassotalea agarivorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Thalassotalea agarivorans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable up to 40°C | Thalassotalea agarivorans |
| 45 | - |
stable up to 45°C | Alteromonas agarilytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Alteromonas agarilytica |
| 8.5 | - |
- |
Thalassotalea agarivorans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | native enzyme | Thalassotalea agarivorans |
| 6.5 | - |
stable at pH values greater 6.5 | Alteromonas agarilytica |
| 6.5 | 10.5 | recombinant enzyme | Thalassotalea agarivorans |
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Release 2023.1 (January 2023)
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