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Literature summary for 3.2.1.158 extracted from
- Hyperproduction and application of alpha-agarase to enzymatic enhancement of antioxidant activity of porphyran (2006), J. Agric. Food Chem., 54, 9895-9900.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli HB101, open reading frame of 1463 amino acid residues of the agaA33 gene determined, for extracellular production of recombinant alpha-agarase AgaA33 the Bacillus subtilis strain ISW1214 serves as host and plasmid pEXBS as expression vector | Thalassotalea agarivorans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 86780 | - |
deduced from amino acid sequence | Thalassotalea agarivorans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | Thalassotalea agarivorans | - |
agarotetraose + ? | - |
? |  |
| agarose + H2O | Thalassotalea agarivorans JAMB-A33 | - |
agarotetraose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thalassotalea agarivorans | A1IGV8 | - |
- |
| Thalassotalea agarivorans JAMB-A33 | A1IGV8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration | Thalassotalea agarivorans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
0.1 U/mL alpha-agarase used for enzymatic cleavage of alpha-1,3 linkages in porphyran at a concentration of 2.0% in 50 mM Tris/HCl buffer for 3 h, specific activity of 44.7 U/mg comparable between recombinant protein and native enzyme purified from Thalassomonas sp. | Thalassotalea agarivorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | - |
Thalassotalea agarivorans | agarotetraose + ? | - |
? | |
| agarose + H2O | hydrolysis of alpha-1,3 linkages, recombinant protein | Thalassotalea agarivorans | agarotetraose + ? | main product agarotetratose corresponds to 79% of total products, concomitant production of agarohexaose (7%), agarobiose (9%), and further agaro-oligosaccharides with different degrees of polymerization (5%) determined, recombinant protein and native protein | ? | |
| agarose + H2O | - |
Thalassotalea agarivorans JAMB-A33 | agarotetraose + ? | - |
? | |
| agarose + H2O | hydrolysis of alpha-1,3 linkages, recombinant protein | Thalassotalea agarivorans JAMB-A33 | agarotetraose + ? | main product agarotetratose corresponds to 79% of total products, concomitant production of agarohexaose (7%), agarobiose (9%), and further agaro-oligosaccharides with different degrees of polymerization (5%) determined, recombinant protein and native protein | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-agarase | - |
Thalassotalea agarivorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
porphyran cleavage assay at | Thalassotalea agarivorans |
| 45 | - |
in 50 mM TAPS containing 1 mM CaCl2, recombinant protein | Thalassotalea agarivorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
porphyran cleavage assay at | Thalassotalea agarivorans |
| 8.5 | - |
recombinant protein | Thalassotalea agarivorans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9.5 | recombinant protein | Thalassotalea agarivorans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10.5 | recombinant protein, more than 75% of original activity retained | Thalassotalea agarivorans |
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