Cloned (Comment) | Organism |
---|---|
agaA gene isolated from a genomic library of Alteromonas agarilytica, alpha-agarase precursor of 1429 amino acid residues, expressed in Escherichia coli, novel catalytic domain defines a new GH family | Alteromonas agarilytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
154000 | - |
deduced from amino acid sequence of pre-protein of 1429 residues | Alteromonas agarilytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
agarose + H2O | Alteromonas agarilytica | - |
agarotetraose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alteromonas agarilytica | Q9LAP7 | alpha-agarase precursor | - |
Purification (Comment) | Organism |
---|---|
- |
Alteromonas agarilytica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Alteromonas agarilytica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
degradation of agarose observed after cultivation of transformed Escherichia coli cells harbouring the agaA gene of Alteromonas agarilytica on Zd agar broth at 22°C | Alteromonas agarilytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agarose + H2O | - |
Alteromonas agarilytica | agarotetraose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | includes the peptides A and B, signal peptide of 26 residues, cleaved between A26 and E27 | Alteromonas agarilytica |
Synonyms | Comment | Organism |
---|---|---|
alpha-agarase | - |
Alteromonas agarilytica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
cultivation of Eschericha coli cells harbouring the agaA gene, agarose lysis observed after one week on Zd agar broth | Alteromonas agarilytica |