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Literature summary for 3.2.1.157 extracted from
- Efficient production of a recombinant iota-carrageenase in Brevibacillus choshinensis using a new integrative vector for the preparation of iota-carrageenan oligosaccharides (2019), Process Biochem., 76, 68-76 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| synthetic gene CGIOP, locus MF459059 in GenBank, codon-optimized gene encoding the iota-carrageenase without a signal peptide, cloned from gene cgiA of Microbulbifer thermotolerans strain JAMB-A94, construction of the integrative vector pBCGA, recombinant expression of extracellular His-tagged enzyme in Brevibacillus choshinensis strain HPD31-SP3, real-time PCR expression analysis, copy numbers of the CGIOP gene in different lines are approximately 5-6, respectively | Microbulbifer thermotolerans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 87% inhibition at 1 mM | Microbulbifer thermotolerans |  |
| EDTA | 30% inhibition at 1 mM | Microbulbifer thermotolerans | |
| Fe2+ | 18% inhibition at 1 mM | Microbulbifer thermotolerans | |
| Fe3+ | 96% inhibition at 1 mM | Microbulbifer thermotolerans | |
| K+ | 25% inhibition at 1 mM | Microbulbifer thermotolerans | |
| Mn2+ | 69% inhibition at 1 mM | Microbulbifer thermotolerans | |
| SDS | 96% inhibition at 1 mM | Microbulbifer thermotolerans | |
| Zn2+ | 95.5% inhibition at 1 mM | Microbulbifer thermotolerans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates 53% at 1 mM | Microbulbifer thermotolerans | |
| Mg2+ | activates 83% at 1 mM | Microbulbifer thermotolerans | |
| NaCl | absolutely required for activity, maximal activity at 0.2 M, about 70% of maximal activity at 0.1 M, 60% at 1.0 M | Microbulbifer thermotolerans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| iota-carrageenan + H2O | Microbulbifer thermotolerans | - |
iota-carrahexaose + iota-carratetraose + iota-carrabiose | - |
? | |
| iota-carrageenan + H2O | Microbulbifer thermotolerans JAMB-A94 | - |
iota-carrahexaose + iota-carratetraose + iota-carrabiose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Microbulbifer thermotolerans | E3W9G3 | - |
- |
| Microbulbifer thermotolerans JAMB-A94 | E3W9G3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Brevibacillus choshinensis strain HPD31-SP3 by dialysis, nickel affinity chromatography, and gel filtration | Microbulbifer thermotolerans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 124.55 | - |
purified recombinant enzyme, pH 8.0, 45°C | Microbulbifer thermotolerans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| iota-carrageenan + H2O | - |
Microbulbifer thermotolerans | iota-carrahexaose + iota-carratetraose + iota-carrabiose | - |
? | |
| iota-carrageenan + H2O | - |
Microbulbifer thermotolerans JAMB-A94 | iota-carrahexaose + iota-carratetraose + iota-carrabiose | - |
? | |
| additional information | analysis of hydrolysis products by thin layer chromatography, the endohydrolytic activity produces low molecular weight iota-carrageenan oligomers. The oligosaccharides observed include iota-carrageenan disaccharide (Dp2), tetrasaccharide (Dp4), and hexasaccharide (Dp6) | Microbulbifer thermotolerans | ? | - |
? | |
| additional information | analysis of hydrolysis products by thin layer chromatography, the endohydrolytic activity produces low molecular weight iota-carrageenan oligomers. The oligosaccharides observed include iota-carrageenan disaccharide (Dp2), tetrasaccharide (Dp4), and hexasaccharide (Dp6) | Microbulbifer thermotolerans JAMB-A94 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 58000, recombinant enzyme, SDS-PAGE | Microbulbifer thermotolerans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CgiA | - |
Microbulbifer thermotolerans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
recombinant enzyme | Microbulbifer thermotolerans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | activity range, recombinant enzyme, profile overview | Microbulbifer thermotolerans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified recombinant enzyme, stable up to, inactivation at 50°C | Microbulbifer thermotolerans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
recombinant enzyme | Microbulbifer thermotolerans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | activity range, recombinant enzyme, profile overview | Microbulbifer thermotolerans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | purified recombinant enzyme, over 60% activity remaining | Microbulbifer thermotolerans |
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