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Literature summary for 3.2.1.157 extracted from
- Cloning, expression and characterization of a iota-carrageenase from marine bacterium Wenyingzhuangia fucanilytica A biocatalyst for producing iota-carrageenan oligosaccharides (2017), J. Biotechnol., 259, 103-109 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme can be utilized as a potential biocatalyst for producing iota-carrageenan oligosaccharides with different polymerization degrees | Wenyingzhuangia fucanilytica |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cgi82A, recombinant expression of His6-tagged enzyme in Escherichia coli | Wenyingzhuangia fucanilytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 23% inhibition at 1 mM | Wenyingzhuangia fucanilytica |  |
| Ca2+ | 6% inhibition at 1 mM | Wenyingzhuangia fucanilytica | |
| Cd2+ | 26% inhibition at 1 mM | Wenyingzhuangia fucanilytica | |
| EDTA | 43% inhibition at 1 mM | Wenyingzhuangia fucanilytica | |
| Hg2+ | 91% inhibition at 1 mM | Wenyingzhuangia fucanilytica | |
| KCl | activates at 0.1 M, inhibitory at over 0.3 M | Wenyingzhuangia fucanilytica | |
| Mn2+ | 13% inhibition at 1 mM | Wenyingzhuangia fucanilytica | |
| NaCl | activates at 0.1 M, inhibitory at over 0.3 M | Wenyingzhuangia fucanilytica | |
| SDS | 87% inhibition at 1 mM | Wenyingzhuangia fucanilytica | |
| Zn2+ | 64% inhibition at 1 mM | Wenyingzhuangia fucanilytica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Wenyingzhuangia fucanilytica | |
| 0.00112 | - |
iota-carrageenan | pH 8.0, 30°C, recombinant enzyme | Wenyingzhuangia fucanilytica |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Wenyingzhuangia fucanilytica | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | activates at 0.1 M, inhibitory at over 0.3 M | Wenyingzhuangia fucanilytica | |
| additional information | poor effect by Mg2+ | Wenyingzhuangia fucanilytica | |
| NaCl | activates at 0.1 M, inhibitory at over 0.3 M | Wenyingzhuangia fucanilytica | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| iota-carrageenan + H2O | Wenyingzhuangia fucanilytica | - |
iota-carratetraose + iota-carrabiose | - |
? | |
| iota-carrageenan + H2O | Wenyingzhuangia fucanilytica CZ1127 | - |
iota-carratetraose + iota-carrabiose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Wenyingzhuangia fucanilytica | A0A1B1Y261 | - |
- |
| Wenyingzhuangia fucanilytica CZ1127 | A0A1B1Y261 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography | Wenyingzhuangia fucanilytica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.55 | - |
substrate iota-carrageenan, pH 8.0, 30°C, recombinant enzyme | Wenyingzhuangia fucanilytica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified recombinant His-tagged enzyme, 84% of initial activity remains after 30 days storage at 4°C without any protective additives | Wenyingzhuangia fucanilytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| iota-carrageenan + H2O | - |
Wenyingzhuangia fucanilytica | iota-carratetraose + iota-carrabiose | - |
? | |
| iota-carrageenan + H2O | commercial substrate type II, the major end product is iota-carrageenan tetrasaccharide, hydrolytic pattern analysis by mass spectrometry | Wenyingzhuangia fucanilytica | iota-carratetraose + iota-carrabiose | - |
? | |
| iota-carrageenan + H2O | - |
Wenyingzhuangia fucanilytica CZ1127 | iota-carratetraose + iota-carrabiose | - |
? | |
| iota-carrageenan + H2O | commercial substrate type II, the major end product is iota-carrageenan tetrasaccharide, hydrolytic pattern analysis by mass spectrometry | Wenyingzhuangia fucanilytica CZ1127 | iota-carratetraose + iota-carrabiose | - |
? | |
| additional information | the recombinant enzyme exhibits slight effects on kappa-carrageenan (0.27 U/mg) and lambda-carrageenan (0.52 U/mg), those activities are two orders lower than that on iota-carrageenan, and may be attributed to the iota-carrageenan impurity in kappa-carrageenan and lambda-carrageenan substrates | Wenyingzhuangia fucanilytica | ? | - |
? | |
| additional information | the recombinant enzyme exhibits slight effects on kappa-carrageenan (0.27 U/mg) and lambda-carrageenan (0.52 U/mg), those activities are two orders lower than that on iota-carrageenan, and may be attributed to the iota-carrageenan impurity in kappa-carrageenan and lambda-carrageenan substrates | Wenyingzhuangia fucanilytica CZ1127 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, recombinant His-tagged enzyme, SDS-PAGE, x * 57196, sequence calculation | Wenyingzhuangia fucanilytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AXE80_00300 | - |
Wenyingzhuangia fucanilytica |
| Cgi82A | - |
Wenyingzhuangia fucanilytica |
| GH82 iota-carrageenase | - |
Wenyingzhuangia fucanilytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Wenyingzhuangia fucanilytica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
purified recombinant His-tagged enzyme, stable up to, 97% and 84% of the initial activity is retained after 24 h at 4°C and 25°C | Wenyingzhuangia fucanilytica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 560.75 | - |
iota-carrageenan | pH 8.0, 30°C, recombinant enzyme | Wenyingzhuangia fucanilytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Wenyingzhuangia fucanilytica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | purified recombinant His-tagged enzyme, stable at | Wenyingzhuangia fucanilytica |
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