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Literature summary for 3.2.1.157 extracted from
- Cloning and characterization of a new cold-adapted and thermo-tolerant iota-carrageenase from marine bacterium Flavobacterium sp. YS-80-122 (2017), Int. J. Biol. Macromol., 102, 1059-1065 .
Application
| Application | Comment | Organism |
|---|---|---|
| industry | CgiF is an excellent candidate for industrial applications in production of iota-carrageen oligosaccharides from seaweed polysaccharides | Flavobacterium sp. YS-80-122 |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cgiF, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Flavobacterium sp. YS-80-122 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | - |
Flavobacterium sp. YS-80-122 |  |
| Cu2+ | - |
Flavobacterium sp. YS-80-122 | |
| EDTA | - |
Flavobacterium sp. YS-80-122 | |
| Fe3+ | - |
Flavobacterium sp. YS-80-122 | |
| Li+ | - |
Flavobacterium sp. YS-80-122 | |
| Mn2+ | - |
Flavobacterium sp. YS-80-122 | |
| NH4+ | - |
Flavobacterium sp. YS-80-122 | |
| SDS | - |
Flavobacterium sp. YS-80-122 | |
| Zn2+ | - |
Flavobacterium sp. YS-80-122 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Flavobacterium sp. YS-80-122 | |
| K+ | activates | Flavobacterium sp. YS-80-122 | |
| Mg2+ | activates | Flavobacterium sp. YS-80-122 | |
| NaCl | activates | Flavobacterium sp. YS-80-122 | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| iota-carrageenan + H2O | Flavobacterium sp. YS-80-122 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Flavobacterium sp. YS-80-122 | A0A1W5QBK3 | isolated from the Yellow Sea sediment | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Flavobacterium sp. YS-80-122 |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:iota-carrageen-grown cell | - |
Flavobacterium sp. YS-80-122 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1207.8 | - |
purified recombinant enzyme, pH 7.6, 30°C | Flavobacterium sp. YS-80-122 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| iota-carrageenan + H2O | - |
Flavobacterium sp. YS-80-122 | ? | - |
? | |
| iota-carrageenan + H2O | CgiF is an endo-type iota-carrageenase that hydrolyzes beta-1,4-linkages of iota-carrageenan, yielding neo-iota-carratetraose as the main product | Flavobacterium sp. YS-80-122 | neo-iota-carratetraose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, recombinant His-tagged enzyme, SDS-PAGE, x * 51000, aboout, sequence calculation | Flavobacterium sp. YS-80-122 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cgiF | - |
Flavobacterium sp. YS-80-122 |
| endo-type iota-carrageenase | - |
Flavobacterium sp. YS-80-122 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Flavobacterium sp. YS-80-122 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 50 | activity range, CgiF is a cold-adapted iota-carrageenase with 36.5% and 57% of maximal activity at 10°C and 15°C, respectively, maximal activity at 30°C, profile overview | Flavobacterium sp. YS-80-122 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
50 | over 80% activity remaining at 0-30°C, 5-10% at 50°C | Flavobacterium sp. YS-80-122 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Flavobacterium sp. YS-80-122 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10.6 | activity range, profile overview | Flavobacterium sp. YS-80-122 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycosyl hydrolase family 82, GH82 | Flavobacterium sp. YS-80-122 |
| physiological function | iota-carrageenases play a role in marine iota-carrageenan degradation, and their enzymatic hydrolysatesare thought to be excellent antioxidants | Flavobacterium sp. YS-80-122 |
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