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Literature summary for 3.2.1.157 extracted from
- Fermentation optimization and enzyme characterization of a new iota-carrageenase from Pseudoalteromonas carrageenovora ASY5 (2018), Electron. J. Biotechnol., 32, 26-34 .
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | activates by 80% at 1 mM | Pseudoalteromonas carrageenovora |  |
| CTAB | activates by 20% at 1 mM | Pseudoalteromonas carrageenovora | |
| DTT | activates by 10% at 1 mM | Pseudoalteromonas carrageenovora | |
| Triton X-100 | activates by 24% at 1 mM | Pseudoalteromonas carrageenovora | |
| Tween 80 | activates by 16% at 1 mM | Pseudoalteromonas carrageenovora | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | complete inhibition at 1 mM | Pseudoalteromonas carrageenovora | |
| Fe3+ | inhibits by 28% at 1 mM | Pseudoalteromonas carrageenovora | |
| K+ | activates at up to 7 mM, inhibitory above | Pseudoalteromonas carrageenovora | |
| Na+ | activates at up to 700 mM, inhibitory above | Pseudoalteromonas carrageenovora | |
| PMSF | 47.5% inhibition at 1 mM | Pseudoalteromonas carrageenovora | |
| SDS | complete inhibition at 1 mM | Pseudoalteromonas carrageenovora | |
| Tween 20 | 30% inhibition at 1 mM | Pseudoalteromonas carrageenovora | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | activates by 20% at 1 mM | Pseudoalteromonas carrageenovora | |
| Al3+ | activates by 10% at 1 mM | Pseudoalteromonas carrageenovora | |
| Ba2+ | activates by 111% at 1 mM | Pseudoalteromonas carrageenovora | |
| Ca2+ | activates by 49% at 1 mM | Pseudoalteromonas carrageenovora | |
| Co2+ | activates by 108% at 1 mM | Pseudoalteromonas carrageenovora | |
| Fe2+ | activates by 30% at 1 mM | Pseudoalteromonas carrageenovora | |
| K+ | activates at up to 7 mM, inhibitory above | Pseudoalteromonas carrageenovora | |
| Mn2+ | activates by 37% at 1 mM | Pseudoalteromonas carrageenovora | |
| additional information | poor effect by Li+, Cd2+, and Mg2+ at 1 mM | Pseudoalteromonas carrageenovora | |
| Na+ | activates at up to 700 mM, inhibitory above | Pseudoalteromonas carrageenovora | |
| Zn2+ | activates by 44% at 1 mM | Pseudoalteromonas carrageenovora | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| iota-carrageenan + H2O | Pseudoalteromonas carrageenovora | - |
iota-carratetraose + iota-carrabiose | - |
? | |
| iota-carrageenan + H2O | Pseudoalteromonas carrageenovora CICC 23819 | - |
iota-carratetraose + iota-carrabiose | - |
? | |
| iota-carrageenan + H2O | Pseudoalteromonas carrageenovora ASY5 | - |
iota-carratetraose + iota-carrabiose | - |
? | |
| additional information | Pseudoalteromonas carrageenovora | the supernatant of marine bacterium Pseudoalteromonas carrageenovora ASY5 can degrade iota-carrageenans and kappa-carrageenans (EC 3.2.1.83) | ? | - |
? | |
| additional information | Pseudoalteromonas carrageenovora CICC 23819 | the supernatant of marine bacterium Pseudoalteromonas carrageenovora ASY5 can degrade iota-carrageenans and kappa-carrageenans (EC 3.2.1.83) | ? | - |
? | |
| additional information | Pseudoalteromonas carrageenovora ASY5 | the supernatant of marine bacterium Pseudoalteromonas carrageenovora ASY5 can degrade iota-carrageenans and kappa-carrageenans (EC 3.2.1.83) | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudoalteromonas carrageenovora | - |
- |
- |
| Pseudoalteromonas carrageenovora ASY5 | - |
- |
- |
| Pseudoalteromonas carrageenovora CICC 23819 | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | iota-carrageenase activity increases from 0.34 U/ml to 1.08 U/ml after optimization. Optimal fermentation conditions are 20°C, pH 7.0, incubation time of 40 h, 15 g/l NaCl, 1.5% w/v yeast extract as nitrogen source, and 0.9% w/v iota-carrageenan as carbon source | Pseudoalteromonas carrageenovora | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| iota-carrageenan + H2O | - |
Pseudoalteromonas carrageenovora | iota-carratetraose + iota-carrabiose | - |
? | |
| iota-carrageenan + H2O | disaccharides and tetrasaccharides as main products | Pseudoalteromonas carrageenovora | iota-carratetraose + iota-carrabiose | - |
? | |
| iota-carrageenan + H2O | - |
Pseudoalteromonas carrageenovora CICC 23819 | iota-carratetraose + iota-carrabiose | - |
? | |
| iota-carrageenan + H2O | disaccharides and tetrasaccharides as main products | Pseudoalteromonas carrageenovora CICC 23819 | iota-carratetraose + iota-carrabiose | - |
? | |
| iota-carrageenan + H2O | - |
Pseudoalteromonas carrageenovora ASY5 | iota-carratetraose + iota-carrabiose | - |
? | |
| iota-carrageenan + H2O | disaccharides and tetrasaccharides as main products | Pseudoalteromonas carrageenovora ASY5 | iota-carratetraose + iota-carrabiose | - |
? | |
| additional information | the supernatant of marine bacterium Pseudoalteromonas carrageenovora ASY5 can degrade iota-carrageenans and kappa-carrageenans (EC 3.2.1.83) | Pseudoalteromonas carrageenovora | ? | - |
? | |
| additional information | analysis of hydrolysis products by ESI-mass spectrometry | Pseudoalteromonas carrageenovora | ? | - |
? | |
| additional information | the supernatant of marine bacterium Pseudoalteromonas carrageenovora ASY5 can degrade iota-carrageenans and kappa-carrageenans (EC 3.2.1.83) | Pseudoalteromonas carrageenovora CICC 23819 | ? | - |
? | |
| additional information | analysis of hydrolysis products by ESI-mass spectrometry | Pseudoalteromonas carrageenovora CICC 23819 | ? | - |
? | |
| additional information | the supernatant of marine bacterium Pseudoalteromonas carrageenovora ASY5 can degrade iota-carrageenans and kappa-carrageenans (EC 3.2.1.83) | Pseudoalteromonas carrageenovora ASY5 | ? | - |
? | |
| additional information | analysis of hydrolysis products by ESI-mass spectrometry | Pseudoalteromonas carrageenovora ASY5 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pseudoalteromonas carrageenovora |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | activity range, profile overview. At 35-40°C for 45 min more than 40% of the maximum activity remains | Pseudoalteromonas carrageenovora |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudoalteromonas carrageenovora |
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