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Literature summary for 3.2.1.157 extracted from
- Insight into carrageenases major review of sources, category, property, purification method, structure, and applications (2018), Crit. Rev. Biotechnol., 38, 1261-1276 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cgiA, sequence comparisons and phylogenetic analysis | Microbulbifer thermotolerans |
| gene cgiA, sequence comparisons and phylogenetic analysis | Alteromonas macleodii |
| gene cgiA, sequence comparisons and phylogenetic analysis | Zobellia galactanivorans |
| gene cgiA2, sequence comparisons and phylogenetic analysis | Zobellia galactanivorans |
| gene cgiB, sequence comparisons and phylogenetic analysis | Cellulophaga sp. QY3 |
| gene cgiF, sequence comparisons and phylogenetic analysis | Flavobacterium sp. YS-80-122 |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| X-ray diffraction structure determination and analysis at 1.60 A resolution, PDB ID 1H80 | Alteromonas macleodii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | - |
Cellulophaga sp. QY3 |  |
| Cu2+ | - |
Cellulophaga sp. QY3 | |
| Cu2+ | - |
Flavobacterium sp. YS-80-122 | |
| EDTA | - |
Cellulophaga sp. QY3 | |
| EDTA | - |
Flavobacterium sp. YS-80-122 | |
| EDTA | - |
Microbulbifer thermotolerans | |
| Fe3+ | - |
Cellulophaga sp. QY3 | |
| Hg2+ | - |
Wenyingzhuangia fucanilytica | |
| SDS | - |
Cellulophaga sp. QY3 | |
| SDS | - |
Flavobacterium sp. YS-80-122 | |
| SDS | - |
Wenyingzhuangia fucanilytica | |
| Zn2+ | - |
Cellulophaga sp. QY3 | |
| Zn2+ | - |
Flavobacterium sp. YS-80-122 | |
| Zn2+ | - |
Wenyingzhuangia fucanilytica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00112 | - |
iota-carrageenan | pH 8.0, 25°C | Wenyingzhuangia fucanilytica | |
| 0.00368 | - |
iota-carrageenan | pH 6.5, 45°C | Cellulophaga sp. QY3 |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Microbulbifer thermotolerans | |
| Ca2+ | activates | Flavobacterium sp. YS-80-122 | |
| Ca2+ | activates | Cellulophaga sp. QY3 | |
| Fe2+ | activates | Cellulophaga sp. QY3 | |
| K+ | activates | Flavobacterium sp. YS-80-122 | |
| K+ | activates | Cellulophaga sp. QY3 | |
| Mg2+ | activates | Flavobacterium sp. YS-80-122 | |
| Mg2+ | activates | Cellulophaga sp. QY3 | |
| Mn2+ | activates | Cellulophaga sp. QY3 | |
| Na+ | activates | Microbulbifer thermotolerans | |
| Na+ | activates | Flavobacterium sp. YS-80-122 | |
| Na+ | activates | Wenyingzhuangia fucanilytica | |
| Na+ | activates | Cellulophaga sp. QY3 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46300 | - |
- |
Zobellia galactanivorans |
| 50600 | 55800 | - |
Cellulophaga sp. QY3 |
| 51000 | - |
- |
Flavobacterium sp. YS-80-122 |
| 53400 | - |
- |
Zobellia galactanivorans |
| 55000 | - |
- |
Microbulbifer thermotolerans |
| 55000 | - |
- |
Wenyingzhuangia fucanilytica |
| 55000 | - |
- |
Alteromonas macleodii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| iota-carrageenan + H2O | Zobellia galactanivorans | - |
? | - |
? | |
| iota-carrageenan + H2O | Cellulophaga sp. QY3 | - |
? | - |
? | |
| iota-carrageenan + H2O | Wenyingzhuangia fucanilytica | - |
iota-carrabiose + iota-carratetraose | - |
? | |
| iota-carrageenan + H2O | Alteromonas macleodii | - |
iota-carrahexaose + iota-carratetraose | - |
? | |
| iota-carrageenan + H2O | Zobellia galactanivorans | - |
iota-carrahexaose + iota-carratetraose | - |
? | |
| iota-carrageenan + H2O | Microbulbifer thermotolerans | - |
iota-carratetraose | - |
? | |
| iota-carrageenan + H2O | Flavobacterium sp. YS-80-122 | - |
iota-carratetraose + iota-carrabiose | - |
? | |
| iota-carrageenan + H2O | Zobellia galactanivorans CCUG 47099 | - |
? | - |
? | |
| iota-carrageenan + H2O | Zobellia galactanivorans CCUG 47099 | - |
iota-carrahexaose + iota-carratetraose | - |
? | |
| iota-carrageenan + H2O | Zobellia galactanivorans Dsij | - |
? | - |
? | |
| iota-carrageenan + H2O | Zobellia galactanivorans Dsij | - |
iota-carrahexaose + iota-carratetraose | - |
? | |
| iota-carrageenan + H2O | Zobellia galactanivorans DSM 12802 | - |
? | - |
? | |
| iota-carrageenan + H2O | Zobellia galactanivorans DSM 12802 | - |
iota-carrahexaose + iota-carratetraose | - |
? | |
| iota-carrageenan + H2O | Zobellia galactanivorans CIP 106680 | - |
? | - |
? | |
| iota-carrageenan + H2O | Zobellia galactanivorans CIP 106680 | - |
iota-carrahexaose + iota-carratetraose | - |
? | |
| iota-carrageenan + H2O | Microbulbifer thermotolerans JAMB-A94T | - |
iota-carratetraose | - |
? | |
| iota-carrageenan + H2O | Zobellia galactanivorans NCIMB 13871 | - |
? | - |
? | |
| iota-carrageenan + H2O | Zobellia galactanivorans NCIMB 13871 | - |
iota-carrahexaose + iota-carratetraose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alteromonas macleodii | Q9F5I8 | - |
- |
| Cellulophaga sp. QY3 | R9UQG9 | - |
- |
| Flavobacterium sp. YS-80-122 | A0A1W5QBK3 | - |
- |
| Microbulbifer thermotolerans | E3W9G3 | - |
- |
| Microbulbifer thermotolerans JAMB-A94T | E3W9G3 | - |
- |
| Wenyingzhuangia fucanilytica | A0A1B1Y261 | - |
- |
| Zobellia galactanivorans | D9UAT1 | - |
- |
| Zobellia galactanivorans | Q9F284 | - |
- |
| Zobellia galactanivorans CCUG 47099 | D9UAT1 | - |
- |
| Zobellia galactanivorans CCUG 47099 | Q9F284 | - |
- |
| Zobellia galactanivorans CIP 106680 | D9UAT1 | - |
- |
| Zobellia galactanivorans CIP 106680 | Q9F284 | - |
- |
| Zobellia galactanivorans Dsij | D9UAT1 | - |
- |
| Zobellia galactanivorans Dsij | Q9F284 | - |
- |
| Zobellia galactanivorans DSM 12802 | D9UAT1 | - |
- |
| Zobellia galactanivorans DSM 12802 | Q9F284 | - |
- |
| Zobellia galactanivorans NCIMB 13871 | D9UAT1 | - |
- |
| Zobellia galactanivorans NCIMB 13871 | Q9F284 | - |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-2-O-sulfonato-alpha-D-galactopyranosyl-(1-3)-4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-2-O-sulfonato-alpha-D-galactopyranosyl-(1-3)-4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhyd | inverting mechanism for the degradation of carrageenan by iota-carrageenases via single displacement reactions, overview | Alteromonas macleodii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| iota-carrageenan + H2O | - |
Zobellia galactanivorans | ? | - |
? | |
| iota-carrageenan + H2O | - |
Cellulophaga sp. QY3 | ? | - |
? | |
| iota-carrageenan + H2O | product is NI4 | Cellulophaga sp. QY3 | ? | - |
? | |
| iota-carrageenan + H2O | - |
Zobellia galactanivorans CCUG 47099 | ? | - |
? | |
| iota-carrageenan + H2O | - |
Zobellia galactanivorans Dsij | ? | - |
? | |
| iota-carrageenan + H2O | - |
Zobellia galactanivorans DSM 12802 | ? | - |
? | |
| iota-carrageenan + H2O | - |
Zobellia galactanivorans CIP 106680 | ? | - |
? | |
| iota-carrageenan + H2O | - |
Zobellia galactanivorans NCIMB 13871 | ? | - |
? | |
| iota-carrageenan + H2O | - |
Wenyingzhuangia fucanilytica | iota-carrabiose + iota-carratetraose | - |
? | |
| iota-carrageenan + H2O | - |
Alteromonas macleodii | iota-carrahexaose + iota-carratetraose | - |
? | |
| iota-carrageenan + H2O | - |
Zobellia galactanivorans | iota-carrahexaose + iota-carratetraose | - |
? | |
| iota-carrageenan + H2O | - |
Zobellia galactanivorans CCUG 47099 | iota-carrahexaose + iota-carratetraose | - |
? | |
| iota-carrageenan + H2O | - |
Zobellia galactanivorans Dsij | iota-carrahexaose + iota-carratetraose | - |
? | |
| iota-carrageenan + H2O | - |
Zobellia galactanivorans DSM 12802 | iota-carrahexaose + iota-carratetraose | - |
? | |
| iota-carrageenan + H2O | - |
Zobellia galactanivorans CIP 106680 | iota-carrahexaose + iota-carratetraose | - |
? | |
| iota-carrageenan + H2O | - |
Zobellia galactanivorans NCIMB 13871 | iota-carrahexaose + iota-carratetraose | - |
? | |
| iota-carrageenan + H2O | - |
Microbulbifer thermotolerans | iota-carratetraose | - |
? | |
| iota-carrageenan + H2O | - |
Microbulbifer thermotolerans JAMB-A94T | iota-carratetraose | - |
? | |
| iota-carrageenan + H2O | - |
Flavobacterium sp. YS-80-122 | iota-carratetraose + iota-carrabiose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | enzyme structure analysis and comparisons, overview | Alteromonas macleodii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CgiA | - |
Alteromonas macleodii |
| CgiA | - |
Microbulbifer thermotolerans |
| CgiA | - |
Wenyingzhuangia fucanilytica |
| CgiA | - |
Zobellia galactanivorans |
| cgiA2 | - |
Zobellia galactanivorans |
| cgiB | - |
Cellulophaga sp. QY3 |
| cgiF | - |
Flavobacterium sp. YS-80-122 |
| zobellia_4265 | - |
Zobellia galactanivorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Wenyingzhuangia fucanilytica |
| 30 | - |
- |
Flavobacterium sp. YS-80-122 |
| 45 | - |
- |
Cellulophaga sp. QY3 |
| 55 | - |
- |
Microbulbifer thermotolerans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Cellulophaga sp. QY3 |
| 7 | - |
- |
Microbulbifer thermotolerans |
| 7.6 | - |
- |
Flavobacterium sp. YS-80-122 |
| 8 | - |
- |
Wenyingzhuangia fucanilytica |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycosyl hydrolase family 82, GH82, phylogenetic analysis of carrageenases from different groups, overview | Microbulbifer thermotolerans |
| evolution | the enzyme belongs to the glycosyl hydrolase family 82, GH82, phylogenetic analysis of carrageenases from different groups, overview | Zobellia galactanivorans |
| evolution | the enzyme belongs to the glycosyl hydrolase family 82, GH82, phylogenetic analysis of carrageenases from different groups, overview | Flavobacterium sp. YS-80-122 |
| evolution | the enzyme belongs to the glycosyl hydrolase family 82, GH82, phylogenetic analysis of carrageenases from different groups, overview | Wenyingzhuangia fucanilytica |
| evolution | the enzyme belongs to the glycosyl hydrolase family 82, GH82, phylogenetic analysis of carrageenases from different groups, overview | Cellulophaga sp. QY3 |
| evolution | the enzyme belongs to the glycosyl hydrolase family 82, GH82, phylogenetic analysis of carrageenases from different groups, overview | Alteromonas macleodii |
| additional information | residues R243, R303, and R353 are involved in substrate binding, residues E245, D247, E310, and H281 are catalytic residues. Glu245 acts as a catalytic proton donor in CgiA, and Asp247 acts as the general base that activates the catalytic water molecule. Three other residues are indirectly involved in the activity: Gln222 simultaneously binds the catalytic water molecule and a chloride ion, playing the essential function of structuring the water network in the active site. His281 participates in iota-carrageenan binding and is likely involved in proton trafficking with the proton donor Glu245, and Glu310 stabilizes the substrate intermediate conformation. Active site structure and molecular docking, overview | Alteromonas macleodii |
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