Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Bifidobacterium adolescentis |
expressed in Escherichia coli BL21(DE3)pLysS cells | uncultured bacterium |
expressed in Escherichia coli BL21(DE3)pLysS cells | Halalkalibacterium halodurans |
expressed in Escherichia coli BL21(DE3)pLysS cells | Pseudoalteromonas haloplanktis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium adolescentis | - |
- |
- |
Halalkalibacterium halodurans | - |
- |
- |
Pseudoalteromonas haloplanktis | - |
- |
- |
uncultured bacterium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity column chromatography, HisTrap HP column chromatography, Q Sepharose column chromatography, and SP Sepharose column chromatography | Bifidobacterium adolescentis |
Ni-NTA affinity column chromatography, HisTrap HP column chromatography, Q Sepharose column chromatography, and SP Sepharose column chromatography | uncultured bacterium |
Ni-NTA affinity column chromatography, HisTrap HP column chromatography, Q Sepharose column chromatography, and SP Sepharose column chromatography | Halalkalibacterium halodurans |
Ni-NTA affinity column chromatography, HisTrap HP column chromatography, Q Sepharose column chromatography, and SP Sepharose column chromatography | Pseudoalteromonas haloplanktis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O | - |
Bifidobacterium adolescentis | 2-nitrophenyl beta-D-xylopyranose + Xylbeta(1-4)Xylbeta(1-4)Xylbeta | - |
? | |
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O | - |
Halalkalibacterium halodurans | 2-nitrophenyl beta-D-xylopyranose + Xylbeta(1-4)Xylbeta(1-4)Xylbeta | - |
? | |
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O | - |
Pseudoalteromonas haloplanktis | 2-nitrophenyl beta-D-xylopyranose + Xylbeta(1-4)Xylbeta(1-4)Xylbeta | - |
? | |
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O | the enzyme has a strong preference for hydrolysis of the third xylosidic bond from the non-reducing end of the substrate | uncultured bacterium | 2-nitrophenyl beta-D-xylopyranose + Xylbeta(1-4)Xylbeta(1-4)Xylbeta | - |
? | |
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O | - |
Bifidobacterium adolescentis | 2-nitrophenyl Xylbeta(1-4)Xylbeta + Xylbeta(1-4)Xylbeta(1-4)Xyl | - |
? | |
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O | - |
uncultured bacterium | 2-nitrophenyl Xylbeta(1-4)Xylbeta + Xylbeta(1-4)Xylbeta(1-4)Xyl | - |
? | |
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O | - |
Halalkalibacterium halodurans | 2-nitrophenyl Xylbeta(1-4)Xylbeta + Xylbeta(1-4)Xylbeta(1-4)Xyl | - |
? | |
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O | - |
Pseudoalteromonas haloplanktis | 2-nitrophenyl Xylbeta(1-4)Xylbeta + Xylbeta(1-4)Xylbeta(1-4)Xyl | - |
? | |
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O | the enzyme cleaves the substrate exclusively at the third xylosidic bond from the reducing end | uncultured bacterium | 2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta + Xylbeta(1-4)Xylbeta(1-4)Xyl | - |
? | |
2-nitrophenyl xylohexaose + H2O | - |
Bifidobacterium adolescentis | ? | - |
? | |
2-nitrophenyl xylohexaose + H2O | - |
Halalkalibacterium halodurans | ? | - |
? | |
2-nitrophenyl xylohexaose + H2O | the enzyme hydrolyzes 2-nitrophenyl xylohexaose mainly at the second and third xylosidic bonds from the reducing end of the substrate | Pseudoalteromonas haloplanktis | ? | - |
? | |
additional information | no activity towards xyloheptaose, xylooctaose, and xylononaose | Halalkalibacterium halodurans | ? | - |
? | |
additional information | no activity towards xylotriose and xylotetraose | uncultured bacterium | ? | - |
? | |
additional information | no activity with xylotriose, xylotetraose, and xylopentaose | Pseudoalteromonas haloplanktis | ? | - |
? | |
oat spelt xylan + H2O | - |
uncultured bacterium | ? | - |
? | |
oat spelt xylan + H2O | - |
Pseudoalteromonas haloplanktis | ? | - |
? | |
soluble oat spelt xylan + H2O | - |
uncultured bacterium | ? | - |
? | |
soluble oat spelt xylan + H2O | - |
Pseudoalteromonas haloplanktis | ? | - |
? | |
wheat flour arabinoxylan + H2O | - |
uncultured bacterium | ? | - |
? | |
wheat flour arabinoxylan + H2O | - |
Pseudoalteromonas haloplanktis | ? | - |
? | |
xyloheptaose + H2O | - |
uncultured bacterium | ? | - |
? | |
xyloheptaose + H2O | - |
Pseudoalteromonas haloplanktis | ? | - |
? | |
xylohexaose + H2O | - |
uncultured bacterium | ? | - |
? | |
xylohexaose + H2O | - |
Halalkalibacterium halodurans | ? | - |
? | |
xylohexaose + H2O | - |
Pseudoalteromonas haloplanktis | ? | - |
? | |
xylononaose + H2O | - |
uncultured bacterium | ? | - |
? | |
xylononaose + H2O | - |
Pseudoalteromonas haloplanktis | ? | - |
? | |
xylooctaose + H2O | - |
uncultured bacterium | ? | - |
? | |
xylooctaose + H2O | - |
Pseudoalteromonas haloplanktis | ? | - |
? | |
xylopentaose + H2O | - |
uncultured bacterium | ? | - |
? | |
xylopentaose + H2O | - |
Halalkalibacterium halodurans | ? | - |
? | |
xylotetraose + H2O | - |
Halalkalibacterium halodurans | ? | - |
? | |
xylotriose + H2O | - |
Halalkalibacterium halodurans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PhXyl | - |
Pseudoalteromonas haloplanktis |
reducing-end xylose-releasing exooligoxylanase | - |
Bifidobacterium adolescentis |
reducing-end xylose-releasing exooligoxylanase | - |
uncultured bacterium |
reducing-end xylose-releasing exooligoxylanase | - |
Halalkalibacterium halodurans |
reducing-end xylose-releasing exooligoxylanase | - |
Pseudoalteromonas haloplanktis |
Rex | - |
Bifidobacterium adolescentis |
RexA | - |
Halalkalibacterium halodurans |
rXyn8 | - |
uncultured bacterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
138 | - |
oat spelt xylan | apparent value, at pH 6.0, 40°C | uncultured bacterium | |
166 | - |
oat spelt xylan | apparent value, at pH 6.0, 40°C | Pseudoalteromonas haloplanktis | |
1099 | - |
wheat flour arabinoxylan | apparent value, at pH 6.0, 40°C | uncultured bacterium | |
1455 | - |
wheat flour arabinoxylan | apparent value, at pH 6.0, 40°C | Pseudoalteromonas haloplanktis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
xylohexaose | apparent value, at pH 6.0, 40°C | Pseudoalteromonas haloplanktis | |
22 | - |
xylopentaose | apparent value, at pH 6.0, 40°C | uncultured bacterium | |
25 | - |
xylohexaose | apparent value, at pH 6.0, 40°C | Halalkalibacterium halodurans | |
30 | - |
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta | apparent value, at pH 6.0, 40°C | Halalkalibacterium halodurans | |
32 | - |
xyloheptaose | apparent value, at pH 6.0, 40°C | Pseudoalteromonas haloplanktis | |
43 | - |
xylotetraose | apparent value, at pH 6.0, 40°C | Halalkalibacterium halodurans | |
65 | - |
xylotriose | apparent value, at pH 6.0, 40°C | Halalkalibacterium halodurans | |
67 | - |
xylooctaose | apparent value, at pH 6.0, 40°C | Pseudoalteromonas haloplanktis | |
92 | - |
xylononaose | apparent value, at pH 6.0, 40°C | Pseudoalteromonas haloplanktis | |
105 | - |
xyloheptaose | apparent value, at pH 6.0, 40°C | uncultured bacterium | |
132 | - |
xylooctaose | apparent value, at pH 6.0, 40°C | uncultured bacterium | |
152 | - |
xylononaose | apparent value, at pH 6.0, 40°C | uncultured bacterium | |
158 | - |
xylohexaose | apparent value, at pH 6.0, 40°C | uncultured bacterium |