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Literature summary for 3.2.1.156 extracted from

  • Pollet, A.; Schoepe, J.; Dornez, E.; Strelkov, S.; Delcour, J.; Courtin, C.
    Functional analysis of glycoside hydrolase family 8 xylanases shows narrow but distinct substrate specificities and biotechnological potential (2010), Appl. Microbiol. Biotechnol., 87, 2125-2135.
    View publication on PubMed
Search for more literature for 3.2.1.156

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Bifidobacterium adolescentis
expressed in Escherichia coli BL21(DE3)pLysS cells uncultured bacterium
expressed in Escherichia coli BL21(DE3)pLysS cells Halalkalibacterium halodurans
expressed in Escherichia coli BL21(DE3)pLysS cells Pseudoalteromonas haloplanktis

Organism

Organism UniProt Comment Textmining
Bifidobacterium adolescentis
-
-
-
Halalkalibacterium halodurans
-
-
-
Pseudoalteromonas haloplanktis
-
-
-
uncultured bacterium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography, HisTrap HP column chromatography, Q Sepharose column chromatography, and SP Sepharose column chromatography Bifidobacterium adolescentis
Ni-NTA affinity column chromatography, HisTrap HP column chromatography, Q Sepharose column chromatography, and SP Sepharose column chromatography uncultured bacterium
Ni-NTA affinity column chromatography, HisTrap HP column chromatography, Q Sepharose column chromatography, and SP Sepharose column chromatography Halalkalibacterium halodurans
Ni-NTA affinity column chromatography, HisTrap HP column chromatography, Q Sepharose column chromatography, and SP Sepharose column chromatography Pseudoalteromonas haloplanktis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O
-
 Bifidobacterium adolescentis 2-nitrophenyl beta-D-xylopyranose + Xylbeta(1-4)Xylbeta(1-4)Xylbeta
-
 ?
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O
-
 Halalkalibacterium halodurans 2-nitrophenyl beta-D-xylopyranose + Xylbeta(1-4)Xylbeta(1-4)Xylbeta
-
 ?
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O
-
 Pseudoalteromonas haloplanktis 2-nitrophenyl beta-D-xylopyranose + Xylbeta(1-4)Xylbeta(1-4)Xylbeta
-
 ?
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O the enzyme has a strong preference for hydrolysis of the third xylosidic bond from the non-reducing end of the substrate uncultured bacterium 2-nitrophenyl beta-D-xylopyranose + Xylbeta(1-4)Xylbeta(1-4)Xylbeta
-
 ?
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O
-
 Bifidobacterium adolescentis 2-nitrophenyl Xylbeta(1-4)Xylbeta + Xylbeta(1-4)Xylbeta(1-4)Xyl
-
 ?
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O
-
 uncultured bacterium 2-nitrophenyl Xylbeta(1-4)Xylbeta + Xylbeta(1-4)Xylbeta(1-4)Xyl
-
 ?
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O
-
 Halalkalibacterium halodurans 2-nitrophenyl Xylbeta(1-4)Xylbeta + Xylbeta(1-4)Xylbeta(1-4)Xyl
-
 ?
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O
-
 Pseudoalteromonas haloplanktis 2-nitrophenyl Xylbeta(1-4)Xylbeta + Xylbeta(1-4)Xylbeta(1-4)Xyl
-
 ?
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O the enzyme cleaves the substrate exclusively at the third xylosidic bond from the reducing end uncultured bacterium 2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta + Xylbeta(1-4)Xylbeta(1-4)Xyl
-
 ?
2-nitrophenyl xylohexaose + H2O
-
 Bifidobacterium adolescentis ?
-
 ?
2-nitrophenyl xylohexaose + H2O
-
 Halalkalibacterium halodurans ?
-
 ?
2-nitrophenyl xylohexaose + H2O the enzyme hydrolyzes 2-nitrophenyl xylohexaose mainly at the second and third xylosidic bonds from the reducing end of the substrate Pseudoalteromonas haloplanktis ?
-
 ?
additional information no activity towards xyloheptaose, xylooctaose, and xylononaose Halalkalibacterium halodurans ?
-
 ?
additional information no activity towards xylotriose and xylotetraose uncultured bacterium ?
-
 ?
additional information no activity with xylotriose, xylotetraose, and xylopentaose Pseudoalteromonas haloplanktis ?
-
 ?
oat spelt xylan + H2O
-
 uncultured bacterium ?
-
 ?
oat spelt xylan + H2O
-
 Pseudoalteromonas haloplanktis ?
-
 ?
soluble oat spelt xylan + H2O
-
 uncultured bacterium ?
-
 ?
soluble oat spelt xylan + H2O
-
 Pseudoalteromonas haloplanktis ?
-
 ?
wheat flour arabinoxylan + H2O
-
 uncultured bacterium ?
-
 ?
wheat flour arabinoxylan + H2O
-
 Pseudoalteromonas haloplanktis ?
-
 ?
xyloheptaose + H2O
-
 uncultured bacterium ?
-
 ?
xyloheptaose + H2O
-
 Pseudoalteromonas haloplanktis ?
-
 ?
xylohexaose + H2O
-
 uncultured bacterium ?
-
 ?
xylohexaose + H2O
-
 Halalkalibacterium halodurans ?
-
 ?
xylohexaose + H2O
-
 Pseudoalteromonas haloplanktis ?
-
 ?
xylononaose + H2O
-
 uncultured bacterium ?
-
 ?
xylononaose + H2O
-
 Pseudoalteromonas haloplanktis ?
-
 ?
xylooctaose + H2O
-
 uncultured bacterium ?
-
 ?
xylooctaose + H2O
-
 Pseudoalteromonas haloplanktis ?
-
 ?
xylopentaose + H2O
-
 uncultured bacterium ?
-
 ?
xylopentaose + H2O
-
 Halalkalibacterium halodurans ?
-
 ?
xylotetraose + H2O
-
 Halalkalibacterium halodurans ?
-
 ?
xylotriose + H2O
-
 Halalkalibacterium halodurans ?
-
 ?

Synonyms

Synonyms Comment Organism
PhXyl
-
 Pseudoalteromonas haloplanktis
reducing-end xylose-releasing exooligoxylanase
-
 Bifidobacterium adolescentis
reducing-end xylose-releasing exooligoxylanase
-
 uncultured bacterium
reducing-end xylose-releasing exooligoxylanase
-
 Halalkalibacterium halodurans
reducing-end xylose-releasing exooligoxylanase
-
 Pseudoalteromonas haloplanktis
Rex
-
 Bifidobacterium adolescentis
RexA
-
 Halalkalibacterium halodurans
rXyn8
-
 uncultured bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
138
-
 oat spelt xylan apparent value, at pH 6.0, 40°C uncultured bacterium
166
-
 oat spelt xylan apparent value, at pH 6.0, 40°C Pseudoalteromonas haloplanktis
1099
-
 wheat flour arabinoxylan apparent value, at pH 6.0, 40°C uncultured bacterium
1455
-
 wheat flour arabinoxylan apparent value, at pH 6.0, 40°C Pseudoalteromonas haloplanktis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5
-
 xylohexaose apparent value, at pH 6.0, 40°C Pseudoalteromonas haloplanktis
22
-
 xylopentaose apparent value, at pH 6.0, 40°C uncultured bacterium
25
-
 xylohexaose apparent value, at pH 6.0, 40°C Halalkalibacterium halodurans
30
-
 2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta apparent value, at pH 6.0, 40°C Halalkalibacterium halodurans
32
-
 xyloheptaose apparent value, at pH 6.0, 40°C Pseudoalteromonas haloplanktis
43
-
 xylotetraose apparent value, at pH 6.0, 40°C Halalkalibacterium halodurans
65
-
 xylotriose apparent value, at pH 6.0, 40°C Halalkalibacterium halodurans
67
-
 xylooctaose apparent value, at pH 6.0, 40°C Pseudoalteromonas haloplanktis
92
-
 xylononaose apparent value, at pH 6.0, 40°C Pseudoalteromonas haloplanktis
105
-
 xyloheptaose apparent value, at pH 6.0, 40°C uncultured bacterium
132
-
 xylooctaose apparent value, at pH 6.0, 40°C uncultured bacterium
152
-
 xylononaose apparent value, at pH 6.0, 40°C uncultured bacterium
158
-
 xylohexaose apparent value, at pH 6.0, 40°C uncultured bacterium