Literature summary for 3.2.1.156 extracted from

Hidaka, M.; Fushinobu, S.; Honda, Y.; Wakagi, T.; Shoun, H.; Kitaoka, M.
Structural explanation for the acquisition of glycosynthase activity (2010), J. Biochem., 147, 237-244.

Search for more literature for 3.2.1.156

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutant enzymes in Escherichia coli strain BL21	Halalkalibacterium halodurans

Crystallization (Commentary)

Crystallization (Comment)	Organism
mutants of reducing-end xylose-releasing exooligoxylanase with glycosynthase activity, X-ray diffraction structure determination and analysis at 1.39-2.10 A resolution	Halalkalibacterium halodurans

Protein Variants

Protein Variants	Comment	Organism
D263C	construction of a mutant engineered reducing-end xylose-releasing exooligoxylanase, that acts as a glycosynthase. Glycosynthases are engineered glycoside hydrolases, that catalyze the synthesis of glycoside from glycosyl-fluoride donors and suitable acceptors, crystal structure determination, overview	Halalkalibacterium halodurans
D263G	construction of a mutant engineered reducing-end xylose-releasing exooligoxylanase, that acts as a glycosynthase. Glycosynthases are engineered glycoside hydrolases, that catalyze the synthesis of glycoside from glycosyl-fluoride donors and suitable acceptors, crystal structure determination, overview	Halalkalibacterium halodurans
D263N	construction of a mutant engineered reducing-end xylose-releasing exooligoxylanase, that acts as a glycosynthase. Glycosynthases are engineered glycoside hydrolases, that catalyze the synthesis of glycoside from glycosyl-fluoride donors and suitable acceptors, crystal structure determination, overview	Halalkalibacterium halodurans
D263S	construction of a mutant engineered reducing-end xylose-releasing exooligoxylanase, that acts as a glycosynthase. Glycosynthases are engineered glycoside hydrolases, that catalyze the synthesis of glycoside from glycosyl-fluoride donors and suitable acceptors, crystal structure determination, overview	Halalkalibacterium halodurans
Y198F	construction of a mutant engineered reducing-end xylose-releasing exooligoxylanase, that acts as a glycosynthase. Glycosynthases are engineered glycoside hydrolases, that catalyze the synthesis of glycoside from glycosyl-fluoride donors and suitable acceptors, crystal structure determination, overview	Halalkalibacterium halodurans

Organism

Organism	UniProt	Comment	Textmining
Halalkalibacterium halodurans	Q9KB30	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the reducing-end xylose-releasing exo-oligoxylanase is an inverting glycohydrolase, active site structure of wild-type enzyme and glycosynthase Rex mutant enzymes, overview	Halalkalibacterium halodurans	?	-	?

Synonyms

Synonyms	Comment	Organism
reducing-end xylose-releasing exo-oligoxylanase	-	Halalkalibacterium halodurans
Rex	-	Halalkalibacterium halodurans

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Release 2023.1 (January 2023)