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Literature summary for 3.2.1.156 extracted from

  • Fushinobu, S.; Hidaka, M.; Honda, Y.; Wakagi, T.; Shoun, H.; Kitaoka, M.
    Structural basis for the specificity of the reducing end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125 (2005), J. Biol. Chem., 280, 17180-17186.
    View publication on PubMed
Search for more literature for 3.2.1.156

Crystallization (Commentary)

Crystallization (Comment) Organism
xylose-enzyme complex is prepared by cocrystallization using a reservoir solution containing 10 mM xylose. The xylobiose complex of the E70A mutant enzyme is prepared by cocrystallization using a reservoir solution containing 10 mM xylotriose Halalkalibacterium halodurans

Protein Variants

Protein Variants Comment Organism
H319A mutation causes a drastic decrease in activity towards the substrate beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose Halalkalibacterium halodurans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0344
-
 beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose mutant enzyme H319A Halalkalibacterium halodurans

Organism

Organism UniProt Comment Textmining
Halalkalibacterium halodurans
-
 C-125
-
Halalkalibacterium halodurans C-125
-
 C-125
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose + ?
-
 Halalkalibacterium halodurans ?
-
 ?
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose + ?
-
 Halalkalibacterium halodurans C-125 ?
-
 ?
additional information the enzyme hydrolyzes xylooligosaccharides whoses degree of polymerization is greater than or equal to 3, releasing the xylose unit at the reducing end Halalkalibacterium halodurans ?
-
 ?
additional information the enzyme hydrolyzes xylooligosaccharides whoses degree of polymerization is greater than or equal to 3, releasing the xylose unit at the reducing end Halalkalibacterium halodurans C-125 ?
-
 ?

Synonyms

Synonyms Comment Organism
reducing end xylose-releasing exo-oligoxylanase
-
 Halalkalibacterium halodurans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21
-
 beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose mutant enzyme H319A Halalkalibacterium halodurans