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Literature summary for 3.2.1.154 extracted from

  • Van Riet, L.; Nagaraj, V.; Van den Ende, W.; Clerens, S.; Wiemken, A.; van Laere, A.
    Purification, cloning and functional characterization of a fructan 6-exohydrolase from wheat (Triticum aestivum L.) (2006), J. Exp. Bot., 57, 213-223.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Triticum aestivum

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by sucrose Triticum aestivum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
1 * 70000, SDS-PAGE Triticum aestivum

Organism

Organism UniProt Comment Textmining
Triticum aestivum Q2UXF7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Triticum aestivum

Source Tissue

Source Tissue Comment Organism Textmining
spike
-
Triticum aestivum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-kestose + H2O as active as levan with native enzyme, 85% of the activity with levan with the heterologous enzyme Triticum aestivum sucrose + D-fructose
-
?
bacterial levan + H2O
-
Triticum aestivum ?
-
?
levanbiose + H2O i.e. O-beta-D-fructofuranosyl-(2-6)-beta-D-fructofuranoside. 63% of the activity with levan with the native enzyme, 57% of the activity with levan with the heterologous enzyme Triticum aestivum beta-D-fructose
-
?
phlein + H2O 95% of the activity levan with native enzyme, 120% of the activity with levan with the heterologous enzyme Triticum aestivum ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 70000, SDS-PAGE Triticum aestivum