Literature summary for 3.2.1.153 extracted from

Asega, A.F.; do Nascimento, J.R.; Schroeven, L.; Van den Ende, W.; Carvalho, M.A.
Cloning, characterization and functional analysis of a 1-FEH cDNA from Vernonia herbacea (Vell.) Rusby (2008), Plant Cell Physiol., 49, 1185-1195.

Search for more literature for 3.2.1.153

Cloned(Commentary)

Cloned (Comment)	Organism
heterologous expression in Pichia pastoris strain X-33 after culturing of plasmids in Escherichia coli, expression vector pPICZalphaA	Vernonia herbacea

Inhibitors

Inhibitors	Comment	Organism	Structure
sucrose	range 0.5-10 mM, 1 mM inhibits 1-FEH by 8%, 5 mM by about 50%, 10 mM by 81%	Vernonia herbacea

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
65700	-	calculated from sequence	Vernonia herbacea

Organism

Organism	UniProt	Comment	Textmining
Vernonia herbacea	A9JIF3	fragment, full-length cDNA of FEH gene of Vernonia herbacea, 2,074 bp, 582 amino acids; samples of sprouting induced plants taken at day 0 and 20, and of defoliated plants stored for one week at 5 degrees Celsius	-

Purification (Commentary)

Purification (Comment)	Organism
concentration by centrifugation after 4 d methanol induction	Vernonia herbacea

Source Tissue

Source Tissue	Comment	Organism	Textmining
rhizophore	transcripts detected in proximal region of sprouting induced plant, in proximal, medium, and distal regions of sprouting induced + 5°C plants	Vernonia herbacea	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	activity increase gradually from 1% up to 8% inulin, however solubility above 5% incomplete	Vernonia herbacea

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-kestose + H2O	from Vernonia herbacea and Vernonia discolor, but levans from Gomphrena macrocephala and Phleum pratense are poorly hydrolyzed, hydrolytic cleavage of terminal fructosyl residues off inulin, sucrose is not hydrolyzed	Vernonia herbacea	D-fructose + sucrose	-	?
inulin + H2O	5% inulin from Vernonia herbacea and Vernonia discolor, but levans from Gomphrena macrocephala and Phleum pratense are poorly hydrolyzed, hydrolytic cleavage of terminal fructosyl residues off inulin, sucrose is not hydrolyzed	Vernonia herbacea	D-fructose + ?	-	?

Synonyms

Synonyms	Comment	Organism
1-FEH	-	Vernonia herbacea
fructan 1-exohydrolase	-	Vernonia herbacea

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Vernonia herbacea

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	50	at pH 5.5, maximal activity of slightly more than 400 microgram fructose per mg protein and h at 40°C, about 95% of maximal activity at 50°C, about 90% of maximal activity at 30°C, about 50% at 20°C, about 25% of maximal activity at 20°C	Vernonia herbacea

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	consistent with a vacuolar or extracellular localization	Vernonia herbacea

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	6.5	0.05M citrate phosphate buffer, 30°C, at pH 5.5 maximal activity of more than 300 microgram fructose per mg protein and hour, at pH 4.5-5.0 about 90% activity, at pH 4.25 and 6.0 about 75% activity, at pH 4.0 and 6.5 about 60% activity	Vernonia herbacea

pI Value

Organism	Comment	pI Value Maximum	pI Value
Vernonia herbacea	estimation by identification and characterization	-	5.32

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)