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Literature summary for 3.2.1.153 extracted from

  • Kusch, U.; Harms, K.; Rausch, T.; Greiner, S.
    Inhibitors of plant invertases do not affect the structurally related enzymes of fructan metabolism (2009), New Phytol., 181, 601-612.
    View publication on PubMed
Search for more literature for 3.2.1.153

Cloned(Commentary)

Cloned (Comment) Organism
48 h in Nicotiana benthamiana leaves infiltrated by Agrobacterium tumefaciens C58C1 with entry vector pDONR(TM)Zeo and destination vector pMDC32 Cichorium intybus

Inhibitors

Inhibitors Comment Organism Structure
additional information proteinaceous inhibitors of acid invertases (structurally related to fructan 1-exohydrolase) do not inhibit fructan 1-exohydrolase Cichorium intybus

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Cichorium intybus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
leaf material frozen, ground, soluble proteins extracted, precipitated, dialyzed, cell-wall associated proteins in pellets incubated in 1 M CaCl, supernatant precipitated, dialysed Cichorium intybus

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Cichorium intybus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0122
-
 of 1-FEH I with 0.0083 micomol/min/mg background activity in Nicotiana benthamiana leaves Cichorium intybus
0.0205
-
 of 1-FEH IIa with 0.0083 micomol/min/mg background activity in Nicotiana benthamiana leaves Cichorium intybus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,1,1-kestopentaose + H2O 1.2 mM in 50 mM Na-acetate buffer, pH 5.2, 0.02% Na-azide, 60 min, 30°C Cichorium intybus D-fructose + ?
-
 ?

Synonyms

Synonyms Comment Organism
1-FEH IIa
-
 Cichorium intybus
fructan 1-exohydrolase
-
 Cichorium intybus