Cloned (Comment) | Organism |
---|---|
gene sav_1856, DNA and amino acid sequence determination and analysis | Streptomyces avermitilis |
gene sav_2574, DNA and amino acid sequence determination and analysis | Streptomyces avermitilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces avermitilis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
x * 78000, recombinant enzyme, SDS.PAGE | Streptomyces avermitilis |
92000 | - |
x * 92000, recombinant enzyme, SDS-PAGE | Streptomyces avermitilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces avermitilis | Q82K30 | gene sav_2574 | - |
Streptomyces avermitilis | Q82M04 | gene sav_1856 | - |
Streptomyces avermitilis DSM 46492 | Q82K30 | gene sav_2574 | - |
Streptomyces avermitilis NBRC 14893 | Q82M04 | gene sav_1856 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme SaGH74A is endo-processive, hydrolysis pattern and mode of action, overview. The C-terminus of enzyme SaGH74A, which is annotated as a carbohydrate-binding module family 2 (CBM2), binds to beta-1,4-linked glucan-containing soluble polysaccharides such as hydroxyethyl cellulose, barley glucan, and xyloglucan, affinities, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan) | Streptomyces avermitilis | ? | - |
? | |
additional information | enzyme SaGH74B is a typical endo-enzyme, hydrolysis pattern and mode of action, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan) | Streptomyces avermitilis | ? | - |
? | |
additional information | enzyme SaGH74A is endo-processive, hydrolysis pattern and mode of action, overview. The C-terminus of enzyme SaGH74A, which is annotated as a carbohydrate-binding module family 2 (CBM2), binds to beta-1,4-linked glucan-containing soluble polysaccharides such as hydroxyethyl cellulose, barley glucan, and xyloglucan, affinities, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan) | Streptomyces avermitilis NBRC 14893 | ? | - |
? | |
additional information | enzyme SaGH74B is a typical endo-enzyme, hydrolysis pattern and mode of action, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan) | Streptomyces avermitilis DSM 46492 | ? | - |
? | |
tamarind xyloglucan + H2O | - |
Streptomyces avermitilis | ? | - |
? | |
tamarind xyloglucan + H2O | - |
Streptomyces avermitilis NBRC 14893 | ? | - |
? | |
tamarind xyloglucan + H2O | - |
Streptomyces avermitilis DSM 46492 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78000, recombinant enzyme, SDS.PAGE | Streptomyces avermitilis |
? | x * 92000, recombinant enzyme, SDS-PAGE | Streptomyces avermitilis |
Synonyms | Comment | Organism |
---|---|---|
SaGH74A | - |
Streptomyces avermitilis |
SaGH74B | - |
Streptomyces avermitilis |
xyloglucanase | - |
Streptomyces avermitilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
recombinant enzyme | Streptomyces avermitilis |
60 | - |
recombinant enzyme | Streptomyces avermitilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | activity range, rapid loss of activity above 60°C, profile overview | Streptomyces avermitilis |
30 | 75 | activity range, profile overview | Streptomyces avermitilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | purified recombinant enzyme, stable at, rapid loss of activity above 50°C, profile overview | Streptomyces avermitilis |
30 | - |
purified recombinant enzyme, stable up to | Streptomyces avermitilis |
40 | - |
purified recombinant enzyme, inactivation | Streptomyces avermitilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
recombinant enzyme | Streptomyces avermitilis |
6 | - |
recombinant enzyme | Streptomyces avermitilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 9.5 | activity range, profile overview | Streptomyces avermitilis |
4 | 7 | activity range, profile overview | Streptomyces avermitilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9 | purified recombinant enzyme, stable at | Streptomyces avermitilis |
6 | 6.5 | purified recombinant enzyme, stable at | Streptomyces avermitilis |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycoside hydrolase family 74, GH74 | Streptomyces avermitilis |