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Literature summary for 3.2.1.151 extracted from
- Isolation and properties of xyloglucanases of Penicillium sp (2010), Biochemistry (Moscow), 75, 41-49.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene xegA, DNA and amino acid sequence determination and analysis | Penicillium canescens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Talaromyces verruculosus | |
| additional information | - |
additional information | kinetics | Penicillium canescens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secredted | Penicillium canescens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
x * 25000, XG 25, SDS-PAGE, x * 70000, XG 70, SDS-PAGE, XG 25 and XG 70 are organized in an enzyme complex | Talaromyces verruculosus |
| 25000 | - |
x * 25000, XG A, SDS-PAGE, x * 26154, XG A, sequence calculation | Penicillium canescens |
| 26154 | - |
x * 25000, XG A, SDS-PAGE, x * 26154, XG A, sequence calculation | Penicillium canescens |
| 70000 | - |
x * 25000, XG 25, SDS-PAGE, x * 70000, XG 70, SDS-PAGE, XG 25 and XG 70 are organized in an enzyme complex | Talaromyces verruculosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xyloglucan + H2O | Talaromyces verruculosus | xyloglucan is a widespread hemicellulose polysaccharide of plant cell walls. It contains mainly D-glucose and D-xylose in the ratio of approximately 4:3, less amount of D-galactose, and also can contain L-fucose and L-arabinose | xyloglucan oligosaccharides | - |
? |  |
| xyloglucan + H2O | Penicillium canescens | xyloglucan is a widespread hemicellulose polysaccharide of plant cell walls. It contains mainly D-glucose and D-xylose in the ratio of approximately 4:3, less amount of D-galactose, and also can contain L-fucose and L-arabinose | xyloglucan oligosaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium canescens | - |
gene xegA encodes isozyme XG A | - |
| Talaromyces verruculosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme from culture medium by anion exchange chromatography | Penicillium canescens |
| native extracellular enzyme from culture medium by anion exchange chromatography to over 95% purity | Talaromyces verruculosus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Penicillium canescens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
comparison with specific activities of other xyloglucanases towards polysaccharide substrates, overview | Talaromyces verruculosus |
| additional information | - |
comparison with specific activities of other xyloglucanases towards polysaccharide substrates, overview | Penicillium canescens |
| 3 | 8 | purified XG 70, substrate is tamarind xyloglucan | Talaromyces verruculosus |
| 21 | - |
purified XG A, substrate is tamarind xyloglucan | Penicillium canescens |
| 52 | - |
purified XG 25, substrate is tamarind xyloglucan | Talaromyces verruculosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is organized in an enzyme complex | Penicillium canescens | ? | - |
? | |
| additional information | XG 25 and XG 70 are organized in an enzyme complex | Talaromyces verruculosus | ? | - |
? | |
| xyloglucan + H2O | xyloglucan is a widespread hemicellulose polysaccharide of plant cell walls. It contains mainly D-glucose and D-xylose in the ratio of approximately 4:3, less amount of D-galactose, and also can contain L-fucose and L-arabinose | Talaromyces verruculosus | xyloglucan oligosaccharides | - |
? | |
| xyloglucan + H2O | xyloglucan is a widespread hemicellulose polysaccharide of plant cell walls. It contains mainly D-glucose and D-xylose in the ratio of approximately 4:3, less amount of D-galactose, and also can contain L-fucose and L-arabinose | Penicillium canescens | xyloglucan oligosaccharides | - |
? | |
| xyloglucan + H2O | tamarind seed xyloglucan | Talaromyces verruculosus | xyloglucan oligosaccharides | - |
? | |
| xyloglucan + H2O | tamarind seed xyloglucan | Penicillium canescens | xyloglucan oligosaccharides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25000, XG 25, SDS-PAGE, x * 70000, XG 70, SDS-PAGE, XG 25 and XG 70 are organized in an enzyme complex | Talaromyces verruculosus |
| ? | x * 25000, XG A, SDS-PAGE, x * 26154, XG A, sequence calculation | Penicillium canescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the glycohydrolase family 12 | Talaromyces verruculosus |
| More | the enzyme belongs to the glycohydrolase family 12 | Penicillium canescens |
| XG 25 | - |
Talaromyces verruculosus |
| XG 70 | - |
Talaromyces verruculosus |
| XG A | - |
Penicillium canescens |
| xyloglucanase | - |
Talaromyces verruculosus |
| xyloglucanase A | - |
Penicillium canescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Talaromyces verruculosus |
| 50 | - |
assay at | Penicillium canescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Talaromyces verruculosus |
| 5 | - |
assay at | Penicillium canescens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Talaromyces verruculosus | XG 70 | - |
3.5 |
| Penicillium canescens | XG A | - |
3.5 |
| Talaromyces verruculosus | XG 25 | - |
4.1 |
| Penicillium canescens | XG A, sequence calculation | - |
4.96 |
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