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Literature summary for 3.2.1.151 extracted from

  • Martinez-Fleites, C.; Guerreiro, C.I.; Baumann, M.J.; Taylor, E.J.; Prates, J.A.; Ferreira, L.M.; Fontes, C.M.; Brumer, H.; Davies, G.J.
    Crystal structures of Clostridium thermocellum xyloglucanase, XGH74A, reveal the structural basis for xyloglucan recognition and degradation (2006), J. Biol. Chem., 281, 24922-24933.
    View publication on PubMed
Search for more literature for 3.2.1.151

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Acetivibrio thermocellus

Crystallization (Commentary)

Crystallization (Comment) Organism
unliganded wild-type and mutant D70A in complex with oligosaccharide substrate Acetivibrio thermocellus

Protein Variants

Protein Variants Comment Organism
D74A crystallization data in complex with oligosaccharide substrate Acetivibrio thermocellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km values are 0.76 mg/ml for xyloglucan, 4.6 mg/ml for carboxymethyl cellulose, 6.6 mg/ml for lichenan, 5.1 mg/ml for carboxyethyl cellulose Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
 isoform XGH74A
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxyethyl cellulose + H2O
-
 Acetivibrio thermocellus carboxyethyl cellulose oligosaccharides
-
 ?
carboxymethyl cellulose + H2O carboxymethyl cellulose-4M Acetivibrio thermocellus carboxymethyl cellulose oligosaccharides
-
 ?
lichenan + H2O
-
 Acetivibrio thermocellus lichenan cellulose oligosaccharides
-
 ?
additional information enzyme does not cleave at substituted glucosyl moieties Acetivibrio thermocellus ?
-
 ?
xyloglucan + H2O source of substrate tamarind seed Acetivibrio thermocellus xyloglucan oligosaccharides
-
 ?

Synonyms

Synonyms Comment Organism
XGH74
-
 Acetivibrio thermocellus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.22
-
 carboxyethyl cellulose pH 7.0, 50°C Acetivibrio thermocellus
0.59
-
 lichenan pH 7.0, 50°C Acetivibrio thermocellus
0.72
-
 carboxymethyl cellulose pH 7.0, 50°C Acetivibrio thermocellus
0.78
-
 xyloglucan pH 7.0, 50°C Acetivibrio thermocellus