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Literature summary for 3.2.1.151 extracted from
- An investigation of the substrate specificity of the xyloglucanase Cel74A from Hypocrea jecorina (2007), FEBS J., 274, 356-363.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km values are 0.8 mg/ml for phosphoric acid-swollen cellulose, 1.3 mg/ml for carboxymethyl cellulose, 4.8 mg/ml for hydroxyethyl cellulose, 2.1 mg/ml for laminarin, 0.5 mg/ml for beta-glucan, 0.3 mg/ml for xyloglucan | Trichoderma reesei | |
| 0.12 | - |
XXXG | 37°C, pH 5 | Trichoderma reesei |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma reesei | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-glucan + H2O | - |
Trichoderma reesei | beta-glucan oligosaccharides | - |
? | |
| carboxymethyl cellulose + H2O | - |
Trichoderma reesei | carboxymethyl cellulose oligosaccharides | - |
? | |
| Glcbeta(1-3)Glcbeta(1-3)Glc + H2O | - |
Trichoderma reesei | D-glucose + Glcbeta(1-3)Glc | 38% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc | ? | |
| Glcbeta(1-4)Glc(1-4)Glc + H2O | - |
Trichoderma reesei | D-glucose + Glcbeta(1-4)Glc | 32% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc | ? | |
| Glcbeta(1-4)Glc(1-4)Glcbeta(1-4)Glc + H2O | - |
Trichoderma reesei | Glcbeta(1-4)Glc | 76% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc | ? | |
| Glcbeta(1-4)Glc-2-chloro-4-nitrophenol + H2O | 24% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc | Trichoderma reesei | D-glucose + 2-chloro-4-nitrophenyl-beta-D-glucoside | - |
? | |
| Glcbeta(1-4)Glcbeta(1-3)Glc + H2O | - |
Trichoderma reesei | D-glucose + Glcbeta(1-3)Glc | 52% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc | ? | |
| Glcbeta(1-4)Glcbeta(1-4)Glc(1-4)Glc-2-chloro-4-nitrophenol + H2O | - |
Trichoderma reesei | Glcbeta(1-4)Glc(1-4)Glc + 2-chloro-4-nitrophenyl-beta-D-glucoside | 84% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc | ? | |
| Glcbeta(1-4)Glcbeta(1-4)Glc-2-chloro-4-nitrophenol + H2O | - |
Trichoderma reesei | Glcbeta(1-4)Glc + 2-chloro-4-nitrophenyl-beta-D-glucoside | 68% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc | ? | |
| Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-3)Glc + H2O | - |
Trichoderma reesei | Glcbeta(1-4)Glc + Glcbeta(1-3)Glc | 83% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc | ? | |
| Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc + H2O | - |
Trichoderma reesei | Glcbeta(1-4)Glcbeta(1-4)Glc + GG | - |
? | |
| hydroxyethyl cellulose + H2O | - |
Trichoderma reesei | hydroxyethyl cellulose oligosaccharides | - |
? | |
| laminarin + H2O | - |
Trichoderma reesei | laminarin oligosaccharides | - |
? | |
| phosphoric acid-swollen cellulose + H2O | - |
Trichoderma reesei | ? | - |
? | |
| XXXG + H2O | X stands for a substituted glucose residue, G for an unsubstituted glucose residue | Trichoderma reesei | XX + XG | 270% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc | ? | |
| XXXGXXXG + H2O | X stands for a substituted glucose residue, G for an unsubstituted glucose residue | Trichoderma reesei | XX + XGXX + XG | hydrolysis at both chain ends of substrate. 390% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc | ? | |
| xyloglucan + H2O | hydrolysis at substituted glucose residues | Trichoderma reesei | xyloglucan oligosaccharides | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cel74A | - |
Trichoderma reesei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Trichoderma reesei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7 | - |
phosphoric acid-swollen cellulose | 37°C, pH 5 | Trichoderma reesei | |
| 7.2 | - |
XXXG | 37°C, pH 5 | Trichoderma reesei | |
| 52 | - |
carboxymethyl cellulose | 37°C, pH 5 | Trichoderma reesei | |
| 138 | - |
hydroxyethyl cellulose | 37°C, pH 5 | Trichoderma reesei | |
| 193 | - |
Laminarin | 37°C, pH 5 | Trichoderma reesei | |
| 306 | - |
beta-Glucan | 37°C, pH 5 | Trichoderma reesei | |
| 805 | - |
xyloglucan | 37°C, pH 5 | Trichoderma reesei | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Trichoderma reesei |
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