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Literature summary for 3.2.1.151 extracted from
- A xyloglucan-specific family 12 glycosyl hydrolase from Aspergillus niger: recombinant expression, purification and characterization (2008), Biochem. J., 411, 161-170.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Aspergillus niger | Aspergillus niger |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km value for xyloglucan is 0.39 mg/ml at 50°C, pH 5.0 | Aspergillus niger |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus niger | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23300 | - |
x * 23300, calculated, mature protein, x * 32000, SDS-PAGE | Aspergillus niger |
| 32000 | - |
x * 23300, calculated, mature protein, x * 32000, SDS-PAGE | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | A1XP58 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | N-glycosylation site at N32 is not glycosylated | Aspergillus niger |
| proteolytic modification | sequence contains a signal peptide of 15 amino acids | Aspergillus niger |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture fluid | - |
Aspergillus niger | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 83 | - |
50°C, pH 5.0 | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethyl curdlan + H2O | - |
Aspergillus niger | carboxymethyl curdlan oligosaccharides | less than 2% of the activity with xyloglucan | ? |  |
| carboxymethyl pachyman + H2O | - |
Aspergillus niger | carboxymethyl pachyman oligosaccharides | less than 2% of the activity with xyloglucan | ? | |
| additional information | no substrate: carboxymethyl cellulose-4M, xylan from birch, beech or oat spelt, lichenan, laminarin, pullulan, galactomannan, glucomannan | Aspergillus niger | ? | - |
? | |
| xyloglucan + H2O | substrate xyloglucan from tamarind seed, cleavage at unbranched glucose in the backbone | Aspergillus niger | xyloglucan oligosaccharides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23300, calculated, mature protein, x * 32000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | - |
Aspergillus niger |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 34 | - |
xyloglucan | 50°C, pH 5.0 | Aspergillus niger | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus niger |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus niger | calculated | - |
3.6 |
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Release 2023.1 (January 2023)
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