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Literature summary for 3.2.1.15 extracted from

  • Cheng, Z.; Chen, D.; Lu, B.; Wei, Y.; Xian, L.; Li, Y.; Luo, Z.; Huang, R.
    A novel acid-stable endo-polygalacturonase from Penicillium oxalicum CZ1028 purification, characterization, and application in the beverage industry (2016), J. Microbiol. Biotechnol., 26, 989-998 .
    View publication on PubMed
Search for more literature for 3.2.1.15

Application

Application Comment Organism
food industry the enzyme significantly reduces the viscosity and increases the light transmittance of papaya pulp, and increases the recovery of the papaya extraction Penicillium oxalicum

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 71.7% residual activity at 2 mM Penicillium oxalicum
Co2+ 70.6% residual activity at 2 mM Penicillium oxalicum
Cu2+ 10.3% residual activity at 2 mM Penicillium oxalicum
Fe2+ 91% residual activity at 2 mM Penicillium oxalicum
Mg2+ 96.5% residual activity at 2 mM Penicillium oxalicum
Mn2+ 40.4% residual activity at 2 mM Penicillium oxalicum
additional information not inhibited by Tween-20, Tween-80, and Triton X-100 Penicillium oxalicum
SDS 30.2% residual activity at 20 mM Penicillium oxalicum
Zn2+ 77.4% residual activity at 2 mM Penicillium oxalicum

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 104.1% activity at 1 mM Penicillium oxalicum
additional information not affected by K+ Penicillium oxalicum
Na+ 110.1% activity at 1 mM Penicillium oxalicum

Organism

Organism UniProt Comment Textmining
Penicillium oxalicum A0A1B1MQM8
-
-
Penicillium oxalicum CZ1028 A0A1B1MQM8
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phenyl Sepharose column chromatography, Q Sepharose column chromatography and Superdex 75 gel filtration Penicillium oxalicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
apple pectin + H2O 8% activity compared to polygalacturonate Penicillium oxalicum ?
-
 ?
apple pectin + H2O 8% activity compared to polygalacturonate Penicillium oxalicum CZ1028 ?
-
 ?
citrus pectin + H2O 28.6% activity compared to polygalacturonate Penicillium oxalicum ?
-
 ?
citrus pectin + H2O 28.6% activity compared to polygalacturonate Penicillium oxalicum CZ1028 ?
-
 ?
additional information no activity with carboxymethyl cellulose and xylan Penicillium oxalicum ?
-
 ?
additional information no activity with carboxymethyl cellulose and xylan Penicillium oxalicum CZ1028 ?
-
 ?
polygalacturonate + H2O 100% activity Penicillium oxalicum oligogalacturonates
-
 ?
polygalacturonate + H2O 100% activity Penicillium oxalicum CZ1028 oligogalacturonates
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Penicillium oxalicum

Synonyms

Synonyms Comment Organism
endo-polygalacturonase
-
 Penicillium oxalicum
EPG
-
 Penicillium oxalicum
EPG4
-
 Penicillium oxalicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 70
-
 Penicillium oxalicum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 more than 60% activity between 40 and 80°C with pectins as substrate Penicillium oxalicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 the enzyme retains 100%, 100%, 80% and 30% activity after 1 h at 40°C, 50°C, 55°C, and 60°C, respectively Penicillium oxalicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Penicillium oxalicum

pH Range

pH Minimum pH Maximum Comment Organism
2.2 7 when tested against pectins, the enzyme shows activity over a broad acidic pH range with more than 15% activity at pH 2.2-6.0 towards citrus pectin; and more than 26.6% activity at pH 2.2-7.0 towards apple pectin Penicillium oxalicum

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 7 the enzyme remains stable after 1 h at pH 2.0-7.0 Penicillium oxalicum