Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.15 extracted from

  • Anand, G.; Yadav, S.; Yadav, D.
    Purification and characterization of polygalacturonase from Aspergillus fumigatus MTCC 2584 and elucidating its application in retting of Crotalaria juncea fiber (2016), 3 Biotech, 6, 201 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.15

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 34% residual activity at 1 mM Aspergillus fumigatus
Ca2+ 29.5% residual activity at 1 mM Aspergillus fumigatus
EDTA 85.7% residual activity at 1 mM Aspergillus fumigatus
Hg2+ 64.2% residual activity at 1 mM Aspergillus fumigatus
additional information KMnO4 has no effect on enzyme activity Aspergillus fumigatus
potassium ferrocyanide 64.2% residual activity at 1 mM Aspergillus fumigatus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 163% activity at 1 mM Aspergillus fumigatus
K+ 135.5% activity at 1 mM Aspergillus fumigatus
additional information Mn2+, Na+, and Zn+ have no effect on enzyme activity Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-
Aspergillus fumigatus MTCC 2584
-
-
-

Purification (Commentary)

Purification (Comment) Organism
acetone precipitation and Sephadex G-100 gel filtration Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polygalacturonate + H2O
-
 Aspergillus fumigatus galacturonate
-
 ?
polygalacturonate + H2O
-
 Aspergillus fumigatus MTCC 2584 galacturonate
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
endo-PG
-
 Aspergillus fumigatus
endo-polygalacturonase
-
 Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Aspergillus fumigatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 about 50% activity at 10°C, about 60% activity at 20°C, 100% activity at 30°C, about 45% activity at 40°C, about 40% activity at 50°C, about 35% activity at 60°C, no activity at 70°C Aspergillus fumigatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 30 the enzyme remains stable for 2 h at 10-30°C and shows about 50% and 25% activity after 2 h at 40°C and 60°C, respectively Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
44
-
 Polygalacturonate at pH 10.0 and 30°C Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Aspergillus fumigatus

pH Range

pH Minimum pH Maximum Comment Organism
8 11 more than 60% activity between pH 8.0 and 11.0, no activity at pH 6.0 and below Aspergillus fumigatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 11 the enzyme remains stable for 2 h at pH 7.0-11.0 Aspergillus fumigatus