Application | Comment | Organism |
---|---|---|
food industry | the enzyme is used for guava juice extraction and clarification. The recovery of juice of enzymatically treated pulp increases from 6% to 23%. Addition of purified enzyme increases the%T650 from 2.5 to 20.4 and °Brix from 1.9 to 4.8. The pH of the enzyme treated juice decreases from 4.5 to 3.02 | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | - |
Aspergillus niger | |
ascorbic acid | - |
Aspergillus niger | |
Ca2+ | - |
Aspergillus niger | |
chlorogenic acid | - |
Aspergillus niger | |
Cinnamic acid | - |
Aspergillus niger | |
Fe2+ | - |
Aspergillus niger | |
ferulic acid | - |
Aspergillus niger | |
K+ | - |
Aspergillus niger | |
L-cystine | - |
Aspergillus niger | |
Mercuric chloride | - |
Aspergillus niger | |
Mn2+ | - |
Aspergillus niger | |
additional information | inhibitory potency in descending order cinnamic acid, ferulic acid, p-coumaric acid, salicylic acid, and chlorgenic acid | Aspergillus niger | |
p-coumaric acid | - |
Aspergillus niger | |
salicylic acid | - |
Aspergillus niger | |
Zn2+ | - |
Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 0.083 mg/ml with polygalacturonic acid at pH 4.8, 45°C | Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | activates | Aspergillus niger | |
Mg2+ | activates | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
1 * 34000 + 1 * 69000, SDS-PAGE | Aspergillus niger |
69000 | - |
1 * 34000 + 1 * 69000, SDS-PAGE | Aspergillus niger |
106000 | - |
native PAGE | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aspergillus niger | the enzyme catalyzes the hydrolytic cleavage of the polygalacturonic acid chain | ? | - |
? | |
additional information | Aspergillus niger MTCC 3323 | the enzyme catalyzes the hydrolytic cleavage of the polygalacturonic acid chain | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger MTCC 3323 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 6.5fold by ethanol precipitation and gel filtration to homogeneity | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
54.3 | - |
determined as micromol/ml/mg protein, purified enzyme, pH 4.8, 45°C, substrate polygalacturonic acid | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Aspergillus niger | ? | - |
? | |
amylopectin + H2O | - |
Aspergillus niger MTCC 3323 | ? | - |
? | |
dextrose agar + H2O | potato dextrose agar, low activity | Aspergillus niger | ? | - |
? | |
dextrose agar + H2O | potato dextrose agar, low activity | Aspergillus niger MTCC 3323 | ? | - |
? | |
additional information | the enzyme catalyzes the hydrolytic cleavage of the polygalacturonic acid chain | Aspergillus niger | ? | - |
? | |
additional information | the enzyme catalyzes the hydrolytic cleavage of the polygalacturonic acid chain | Aspergillus niger MTCC 3323 | ? | - |
? | |
pectin + H2O | citrus or apple pectin | Aspergillus niger | ? | - |
? | |
pectin + H2O | citrus or apple pectin | Aspergillus niger MTCC 3323 | ? | - |
? | |
polygalacturonic acid + H2O | - |
Aspergillus niger | galacturonic acid | - |
? | |
polygalacturonic acid + H2O | - |
Aspergillus niger MTCC 3323 | galacturonic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 34000 + 1 * 69000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
purified enzyme, half-life is 104 h | Aspergillus niger |
25 | - |
purified enzyme, half-life is 3 h | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
- |
Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 6.5 | over 50% of maximal acitivity at pH 3.5-6.0, 32% at pH 6.5 | Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 5.5 | purified enzyme, 1 h, stable at | Aspergillus niger |