Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.15 extracted from

  • Kant, S.; Vohra, A.; Gupta, R.
    Purification and physicochemical properties of polygalacturonase from Aspergillus niger MTCC 3323 (2013), Protein Expr. Purif., 87, 11-16.
    View publication on PubMed

Application

Application Comment Organism
food industry the enzyme is used for guava juice extraction and clarification. The recovery of juice of enzymatically treated pulp increases from 6% to 23%. Addition of purified enzyme increases the%T650 from 2.5 to 20.4 and °Brix from 1.9 to 4.8. The pH of the enzyme treated juice decreases from 4.5 to 3.02 Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
Al3+
-
Aspergillus niger
ascorbic acid
-
Aspergillus niger
Ca2+
-
Aspergillus niger
chlorogenic acid
-
Aspergillus niger
Cinnamic acid
-
Aspergillus niger
Fe2+
-
Aspergillus niger
ferulic acid
-
Aspergillus niger
K+
-
Aspergillus niger
L-cystine
-
Aspergillus niger
Mercuric chloride
-
Aspergillus niger
Mn2+
-
Aspergillus niger
additional information inhibitory potency in descending order cinnamic acid, ferulic acid, p-coumaric acid, salicylic acid, and chlorgenic acid Aspergillus niger
p-coumaric acid
-
Aspergillus niger
salicylic acid
-
Aspergillus niger
Zn2+
-
Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information 0.083 mg/ml with polygalacturonic acid at pH 4.8, 45°C Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ activates Aspergillus niger
Mg2+ activates Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
1 * 34000 + 1 * 69000, SDS-PAGE Aspergillus niger
69000
-
1 * 34000 + 1 * 69000, SDS-PAGE Aspergillus niger
106000
-
native PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Aspergillus niger the enzyme catalyzes the hydrolytic cleavage of the polygalacturonic acid chain ?
-
?
additional information Aspergillus niger MTCC 3323 the enzyme catalyzes the hydrolytic cleavage of the polygalacturonic acid chain ?
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger MTCC 3323
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 6.5fold by ethanol precipitation and gel filtration to homogeneity Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
54.3
-
determined as micromol/ml/mg protein, purified enzyme, pH 4.8, 45°C, substrate polygalacturonic acid Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
Aspergillus niger ?
-
?
amylopectin + H2O
-
Aspergillus niger MTCC 3323 ?
-
?
dextrose agar + H2O potato dextrose agar, low activity Aspergillus niger ?
-
?
dextrose agar + H2O potato dextrose agar, low activity Aspergillus niger MTCC 3323 ?
-
?
additional information the enzyme catalyzes the hydrolytic cleavage of the polygalacturonic acid chain Aspergillus niger ?
-
?
additional information the enzyme catalyzes the hydrolytic cleavage of the polygalacturonic acid chain Aspergillus niger MTCC 3323 ?
-
?
pectin + H2O citrus or apple pectin Aspergillus niger ?
-
?
pectin + H2O citrus or apple pectin Aspergillus niger MTCC 3323 ?
-
?
polygalacturonic acid + H2O
-
Aspergillus niger galacturonic acid
-
?
polygalacturonic acid + H2O
-
Aspergillus niger MTCC 3323 galacturonic acid
-
?

Subunits

Subunits Comment Organism
heterodimer 1 * 34000 + 1 * 69000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
purified enzyme, half-life is 104 h Aspergillus niger
25
-
purified enzyme, half-life is 3 h Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6.5 over 50% of maximal acitivity at pH 3.5-6.0, 32% at pH 6.5 Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 5.5 purified enzyme, 1 h, stable at Aspergillus niger