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Literature summary for 3.2.1.15 extracted from

  • Sandeep, B.; Kishor Mohan, T.; Murugan, K.
    Purification and kinetic characterization of polygalacturonase-3 from palmyrah palm (Borassus flabellifer L) (2014), J. Plant Biochem. Biotechnol., 23, 221-224.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol slight activation at 0.1-0.5 mM Borassus flabellifer
PEG slight activation at 0.1 mM, no more at 0.5 mM Borassus flabellifer

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 12% inhibition at 0.5 mM Borassus flabellifer
Cd2+ 98% inhibition at 0.5 mM Borassus flabellifer
cetyltrimethylammonium bromide 67% inhibition at 0.5% Borassus flabellifer
Cu2+ 23% inhibition at 0.5 mM Borassus flabellifer
Fe2+ 18% inhibition at 0.5 mM Borassus flabellifer
Hg2+ 96% inhibition at 0.5 mM Borassus flabellifer
p-chloromercuribenzoate 80% inhibition at 0.1 mM, 94% at 0.5 mM Borassus flabellifer
Pb2+ 97% inhibition at 0.5 mM Borassus flabellifer
Teepol 43% inhibition at 0.5% Borassus flabellifer
Triton X-100 10% inhibition at 0.5% Borassus flabellifer

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
poly-D-galacturonic acid pH 5.0, 40°C Borassus flabellifer

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates 46% at 0.5 mM Borassus flabellifer
Mg2+ activates 10% at 0.5 mM Borassus flabellifer
additional information no effects by 0.5 mM of Mn2+, Na+, and K+ Borassus flabellifer

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
gel filtration Borassus flabellifer

Organism

Organism UniProt Comment Textmining
Borassus flabellifer
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 100.9fold from palm fruits to homogeneity by concanavalin A affinity chromatography Borassus flabellifer

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Borassus flabellifer
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
322.9
-
purified enzyme, pH 5.0, 40°C Borassus flabellifer

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly-D-galacturonic acid + H2O
-
Borassus flabellifer D-galacturonic acid
-
?

Subunits

Subunits Comment Organism
monomer 1 * 66000, about, SDS-PAGE Borassus flabellifer

Synonyms

Synonyms Comment Organism
PG-3
-
Borassus flabellifer
polygalacturonase-3
-
Borassus flabellifer

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 40
-
Borassus flabellifer

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
purified enzyme, pH 5.0, stable up to Borassus flabellifer
70
-
purified enzyme, pH 5.0, loss of 52% activity Borassus flabellifer
80
-
purified enzyme, pH 5.0, loss of 90% activity Borassus flabellifer

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Borassus flabellifer

pH Range

pH Minimum pH Maximum Comment Organism
4 9 activity range, 19% of maximal activity at pH 9.0 Borassus flabellifer