Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | slight activation at 0.1-0.5 mM | Borassus flabellifer | |
PEG | slight activation at 0.1 mM, no more at 0.5 mM | Borassus flabellifer |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 12% inhibition at 0.5 mM | Borassus flabellifer | |
Cd2+ | 98% inhibition at 0.5 mM | Borassus flabellifer | |
cetyltrimethylammonium bromide | 67% inhibition at 0.5% | Borassus flabellifer | |
Cu2+ | 23% inhibition at 0.5 mM | Borassus flabellifer | |
Fe2+ | 18% inhibition at 0.5 mM | Borassus flabellifer | |
Hg2+ | 96% inhibition at 0.5 mM | Borassus flabellifer | |
p-chloromercuribenzoate | 80% inhibition at 0.1 mM, 94% at 0.5 mM | Borassus flabellifer | |
Pb2+ | 97% inhibition at 0.5 mM | Borassus flabellifer | |
Teepol | 43% inhibition at 0.5% | Borassus flabellifer | |
Triton X-100 | 10% inhibition at 0.5% | Borassus flabellifer |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
poly-D-galacturonic acid | pH 5.0, 40°C | Borassus flabellifer |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates 46% at 0.5 mM | Borassus flabellifer | |
Mg2+ | activates 10% at 0.5 mM | Borassus flabellifer | |
additional information | no effects by 0.5 mM of Mn2+, Na+, and K+ | Borassus flabellifer |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
gel filtration | Borassus flabellifer |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Borassus flabellifer | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 100.9fold from palm fruits to homogeneity by concanavalin A affinity chromatography | Borassus flabellifer |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Borassus flabellifer | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
322.9 | - |
purified enzyme, pH 5.0, 40°C | Borassus flabellifer |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly-D-galacturonic acid + H2O | - |
Borassus flabellifer | D-galacturonic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 66000, about, SDS-PAGE | Borassus flabellifer |
Synonyms | Comment | Organism |
---|---|---|
PG-3 | - |
Borassus flabellifer |
polygalacturonase-3 | - |
Borassus flabellifer |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | - |
Borassus flabellifer |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified enzyme, pH 5.0, stable up to | Borassus flabellifer |
70 | - |
purified enzyme, pH 5.0, loss of 52% activity | Borassus flabellifer |
80 | - |
purified enzyme, pH 5.0, loss of 90% activity | Borassus flabellifer |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Borassus flabellifer |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | activity range, 19% of maximal activity at pH 9.0 | Borassus flabellifer |